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Effect of salinity (Nacl) on seeds germination and seedling growth cultured in laboratory of two cultivars of soybean [Glycine max (L) Merr.]

تأثير الملوحة (Nacl) في إنبات البذور ونمو البادرات المزروعة في المختبر لصنفين من نبات فول الصويا [ Glycin max (L) Merr]

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 Publication date 2014
and research's language is العربية
 Created by Shamra Editor




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The objective of this study is to compare the effect of five different Nacl concentration (0,50,100,150,200 mM) on seeds germination and seedling growth of two cultivars of soybean (sb-44,sb-172). Seeds were germinated in petri dishes with sterile filter paper moistened with 10 ml of test solution at 25 c˚ in dark . The obtained results showed that germination percentage , length of root and hypocotyl were decreased with the increase of the Nacl concentration in the medium. The best values was from cultivar sb-44 . After seven days of incubation , significant differences were observed between control and treated seeds . Germinated seeds were subcultured on basal Murashige and Skoog medium solid at the same Nacl concentration . The roots growth was mor affected by 200 mM Nacl compared to seedling growth . Plantlets were transfered into pots with nutrient soil , irrigated with saline water and were adapted at laboratory . we obtained good plants grew to maturity in 10- 12 weeks .


Artificial intelligence review:
Research summary
تهدف الدراسة إلى مقارنة تأثير خمسة تراكيز مختلفة من الملوحة (Nacl) (0، 50، 100، 150، 200 ميلي مول) على إنبات بذور ونمو بادرات صنفين من نبات فول الصويا (sb-44 وsb-172). تمت زراعة البذور في أطباق بتري تحتوي على ورق ترشيح معقم ومبلل بالمحلول المستخدم، وتمت الحضانة في درجة حرارة 25 م° وفي الظلام. أظهرت النتائج أن نسبة الإنبات وطول الجذر والسويقة تتناقص مع زيادة تركيز Nacl، وكان الصنف sb-44 أفضل من حيث التحمل للملوحة. بعد سبعة أيام من الحضانة، تم نقل البذور النابتة إلى وسط Murashige and Skoog (MS) الصلب الذي يحتوي على نفس تركيز الملوحة. أظهرت النتائج أن نمو الجذور كان أكثر تأثراً بتركيز 200 ميلي مول Nacl مقارنة بنمو البادرات. تم نقل البادرات إلى أصص تحتوي على تربة مغذية وتم ريها بماء ملحي بنفس التركيز، وتمت أقلمتها في المختبر. حصلت الدراسة على نباتات بحالة خضرية جيدة استمرت في النمو حتى مرحلة النضج خلال 10-12 أسبوعاً.
Critical review
دراسة نقدية: تعتبر هذه الدراسة مهمة لأنها تسلط الضوء على تأثير الملوحة على إنبات ونمو نبات فول الصويا، وهو محصول اقتصادي مهم. ومع ذلك، يمكن توجيه بعض الانتقادات البناءة للدراسة. أولاً، كان من الممكن توسيع نطاق الدراسة لتشمل أصناف أخرى من فول الصويا أو حتى نباتات أخرى لتحسين الفهم العام لتأثير الملوحة. ثانياً، كان من الممكن استخدام تقنيات تحليلية أكثر تقدماً لقياس تأثير الملوحة على المستوى الجزيئي، مثل تحليل التعبير الجيني أو البروتيني. ثالثاً، لم تتناول الدراسة بشكل كافٍ التأثيرات البيئية الأخرى التي قد تتفاعل مع الملوحة، مثل درجة الحرارة أو نوعية التربة. وأخيراً، كان من الممكن تقديم توصيات أكثر تفصيلاً للمزارعين حول كيفية إدارة الملوحة في حقولهم بناءً على نتائج الدراسة.
Questions related to the research
  1. ما هو الهدف الرئيسي من الدراسة؟

    الهدف الرئيسي من الدراسة هو مقارنة تأثير خمسة تراكيز مختلفة من الملوحة (Nacl) على إنبات بذور ونمو بادرات صنفين من نبات فول الصويا (sb-44 وsb-172).

  2. ما هي النتائج الرئيسية التي توصلت إليها الدراسة؟

    النتائج الرئيسية هي أن نسبة الإنبات وطول الجذر والسويقة تتناقص مع زيادة تركيز Nacl، وكان الصنف sb-44 أفضل من حيث التحمل للملوحة.

  3. كيف تم إعداد البذور للزراعة في الدراسة؟

    تم نقع البذور في ماء عادي لمدة ساعة، ثم تعقيمها بالكحول الإثيلي 70% لمدة دقيقة واحدة، وبعدها غمرها برشاحة هيبوكلوريت الكالسيوم 8% لمدة 20 دقيقة، ثم غسلها ثلاث مرات بالماء المقطر والمعقم.

  4. ما هي التوصيات التي قدمتها الدراسة؟

    التوصيات تشمل دراسة مقارنة لأصناف أخرى لمعرفة درجة تحملها للملوحة واعتماد الأصناف الأكثر تحملاً للملوحة في الزراعة.


References used
ALMANSO URI,M.; KINET,J.M and LUTT,S. Effect of salt and osmotic stresses on germination in durum wheat. Plant soil, 231, 2001, 243-254
AMIRJANI,M.R. Effect of salinity stress on growth, mineral composition, proline content, antioxidant enzymes of soybean. Amer.J. of plant physiology. 5,2010, 350- 360
ATAK,M..; KAYA, N.D.; CIKILI,Y. and CIFTCI, C.Y. Effect of Nacl on the germination, seedling growth and water uptake of triticale. Turk.J.Agric.for.30,2006,39-47
DASILVA, R.N.; LOPES, N.; DEMORAES, D.; PEREIRA, A.D. and DUARTE, G. Physiological uality of Barley seeds submitted to saline stress. Revista, Brasil. de sements. Vol.29,n˚1,2007,40-44
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The objective of this study is to compare the effect of five different Nacl concentration (0,50,100,150,200 mM) on seeds germination and seedling growth of two cultivars of soybean (sb-44,sb-172). Seeds were germinated in petri dishes with sterile filter paper moistened with 10 ml of test solution at 25 c˚ in dark . The obtained results showed that germination percentage , length of root and hypocotyl were decreased with the increase of the Nacl concentration in the medium. The best values was from cultivar sb-44 . After seven days of incubation , significant differences were observed between control and treated seeds . Germinated seeds were subcultured on basal Murashige and Skoog medium solid at the same Nacl concentration . The roots growth was mor affected by 200 mM Nacl compared to seedling growth . Plantlets were transfered into pots with nutrient soil , irrigated with saline water and were adapted at laboratory . we obtained good plants grew to maturity in 10- 12 weeks .
The aime of this study was to isolate and identify the seed borne fungi of two varieties of Soybean, Sb172 and Sb44, using blotter method and P.D.A plate method. 21 species belong 10 genus were determined, saprophyte and pathogen Fungi. P.D.A plat e method showed the highest number of fungi in comparison with the other method, and the fungus Aspergillus was the most frequent genus with a ratio of 44.2% for the variety sb172 and 45.14% for the variety sb44, followed by the fungus Cladosporium and then Fusarium, whereas the genus Colletotrichum showed the lowest frequent ratio (0.85%) with the variety Sb172. The percentages of seeds germination and infection were differed between the two varieties, and the ratio seeds germination of variety sb172 were higher than the variety sb44 with the both methods of culture, and were equal 94% with prim variety and 92% with the second ones by blotter method, whereas the ratio were 52% and 34% for both varieties respectively by P.D.A plate method. However, the ratio of infection were, in general, lower with prim variety than the second ones by the both method. The results showed the seeds which were highest ratio infection appeared low ratio germination .
The present study was conducted at the First of May Station belongs to the General Commission For Scientific Agricultural Research (GCSAR) at Damascus during 2011 and 2012 growing seasons to estimate gene action, heritability, genotypic and phenot ypic coefficient variations (GCV, PCV) and genetic advance for two crosses of soybean. The five population (P1, P2, F1, F2, F3) of each cross were grown in a randomized complete block design (RCBD) with three replications in order to evaluate oil and protein contents and seed yield traits. Results of mean square analysis showed significant differences (p<0.05) among mean values of the five populations for all studied traits between the populations of each cross. The second cross achieved high broad and narrow sense heritability (66%, 44%) for protein content, high value of phenotypic and genotypic variations in seed yield in the second cross achieved (18.03,17.30) and high value of expected genetic advance was obtained 11.09%. In most traits, epistasis of the dominance × dominance type of gene action occupied the first rank in order of importance in controlling inheritance of the evaluate traits in both crosses indicating the importance of selection in late segregating generations to improve these traits. These findings are supported by high values of heritability, which was related with low to moderate expected genetic advance in two crosses.
This investigation was conducted at the laboratories of Horticulture Departments, Faculty of Agriculture to study the effect of different levels of salinity (NaCl) in the rate and the percentage of seed germination of lettuce, Lactuca sativa L. va rieties: Shinshar and Kabous and to determine the seedling response to these levels of salinity with relative to root length, the length of the cold, fresh and dry weight of seedlings. NaCl was used at 0, 50, 100, 150 and 200 mM/l. Resulted showed that a gradual increase in salinity concentrations caused a gradual decline in all indicators studied in both cultivars. However, the plants of Kabous variety were less sensitive to the salinity and also higher than in the indicators studied for seedlings (rate of germination and percent, stems and roots length, fresh and dry weight of seedlings) under salt stress conditions.
The effect of salinity and the treatment with fungicide on seed germination of Cupressus sempervirens L. was investigated. Five concentrations of salinity (0, 0.5, 1, 5 and 10%) were used. One part of the seeds was treated with fungicides (Benomyl ), while the other part was germinated without fungicide treatment. The results showed that seed germination was reduced by salinity, but the reduction was more pronounced without fungicide and with salt concentration, the seeds didn't germinate at 5 and 10% NaCl. Despite the decrease of germination indices with the increase of salt concentrations, the statistical results didn't show significant differences in salinity concentrations and the control in germination speed without fungicide, but they were significant with the fungicide. The statistical results showed significant differences between the presence and absence of fungicide in all treatments
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