تهدف الدراسة إلى مقارنة تأثير خمسة تراكيز ملحية (Nacl) مختلفة (0، 50، 100، 150، 200 ميلي مول) في إنبات البذور ونمو البادرات لصنفين من نبات فول الصويا (sb-172، sb-44) .
تمّ إنبات البذور في أطباق بتري على أوراق ترشيح معقمة ومرطبة بـ10 ميلي ليتر من الوسط المستخدم؛ وذلك في درجة حرارة 25 م˚ وفي الظلام . أظهرت النتائج التي حصلنا عليها تناقص النسبة المئوية للإنبات , طول الجذر والسويقة مع زيادة تركيز Nacl في الوسط ، وكانت أفضل القيم من الصنف sb-44 .
تمّت ملاحظة فروق معنوية بين الشاهد والبذور المعالجة بعد 7 أيام من الحضانة . زرعت البذور النابتة على وسط Murashige and Skoog الأساسي (MS) والصلب الذي يحتوي التركيز نفسه من الملوحة . كان نمو الجذور أكثر تأثراً بالتركيز 200 ميلي مول Nacl مقارنة بنمو البادرات . نقلت هذه الأخيرة , بعد ذلك , إلى أصص تحوي تربة مغذية (تورب), ورويت بماء ملحي (التركيز نفسه الذي نمت عليه), وتمت أقلمتها في المختبر .
حصلنا على نباتات بحالة خضرية جيدة, استمر نموها إلى مرحلة النضج خلال 12-10 أسبوعاً.
The objective of this study is to compare the effect of five different Nacl concentration (0,50,100,150,200 mM) on seeds germination and seedling growth of two cultivars of soybean (sb-44,sb-172).
Seeds were germinated in petri dishes with sterile filter paper moistened with 10 ml of test solution at 25 c˚ in dark .
The obtained results showed that germination percentage , length of root and hypocotyl were decreased with the increase of the Nacl concentration in the medium. The best values was from cultivar sb-44 .
After seven days of incubation , significant differences were observed between control and treated seeds .
Germinated seeds were subcultured on basal Murashige and Skoog medium solid at the same Nacl concentration . The roots growth was mor affected by 200 mM Nacl compared to seedling growth .
Plantlets were transfered into pots with nutrient soil , irrigated with saline water and were adapted at laboratory . we obtained good plants grew to maturity in 10- 12 weeks .
References used
ALMANSO URI,M.; KINET,J.M and LUTT,S. Effect of salt and osmotic stresses on germination in durum wheat. Plant soil, 231, 2001, 243-254
AMIRJANI,M.R. Effect of salinity stress on growth, mineral composition, proline content, antioxidant enzymes of soybean. Amer.J. of plant physiology. 5,2010, 350- 360
ATAK,M..; KAYA, N.D.; CIKILI,Y. and CIFTCI, C.Y. Effect of Nacl on the germination, seedling growth and water uptake of triticale. Turk.J.Agric.for.30,2006,39-47
DASILVA, R.N.; LOPES, N.; DEMORAES, D.; PEREIRA, A.D. and DUARTE, G. Physiological uality of Barley seeds submitted to saline stress. Revista, Brasil. de sements. Vol.29,n˚1,2007,40-44
The objective of this study is to compare the effect of five different Nacl
concentration (0,50,100,150,200 mM) on seeds germination and seedling growth of two
cultivars of soybean (sb-44,sb-172).
Seeds were germinated in petri dishes with sterile
The aime of this study was to isolate and identify the seed borne fungi of two
varieties of Soybean, Sb172 and Sb44, using blotter method and P.D.A plate method.
21 species belong 10 genus were determined, saprophyte and pathogen Fungi.
P.D.A plat
The present study was conducted at the First of May Station belongs to the
General Commission For Scientific Agricultural Research (GCSAR) at
Damascus during 2011 and 2012 growing seasons to estimate gene action,
heritability, genotypic and phenot
This investigation was conducted at the laboratories of Horticulture
Departments, Faculty of Agriculture to study the effect of different levels of
salinity (NaCl) in the rate and the percentage of seed germination of lettuce,
Lactuca sativa L. va
The effect of salinity and the treatment with fungicide on seed germination of Cupressus
sempervirens L. was investigated.
Five concentrations of salinity (0, 0.5, 1, 5 and 10%) were used. One part of the seeds was
treated with fungicides (Benomyl