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Register a new userEffect of salinity (Nacl) on seeds germination and seedling growth cultured in laboratory of two cultivars of soybean [Glycine max (L) Merr.]
تأثير الملوحة (Nacl) في إنبات البذور و نمو البادرات المزروعة في المختبر لصنفين من نبات فول الصويا [ Glycin max (L.) Merr. ]
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Tishreen University ورقة بحثية
Publication date
2014
fields
Biology
and research's language is
العربية
Authors
دانيال العوض( باحث )
Created by
Shamra Editor
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تهدف الدراسة إلى مقارنة تأثير خمسة تراكيز ملحية (Nacl) مختلفة (0، 50، 100، 150، 200 ميلي مول) في إنبات البذور و نمو البادرات لصنفين من نبات فول الصويا (sb-172، sb-44) . تمّ إنبات البذور في أطباق بتري على أوراق ترشيح معقمة و مرطبة بـ10 ميلي ليتر من الوسط المستخدم؛ و ذلك في درجة حرارة 25 م˚ و في الظلام . أظهرت النتائج التي حصلنا عليها تناقص النسبة المئوية للإنبات , طول الجذر و السويقة مع زيادة تركيز Nacl في الوسط ، و كانت أفضل القيم من الصنف sb-44 . تمّت ملاحظة فروق معنوية بين الشاهد و البذور المعالجة بعد 7 أيام من الحضانة . زرعت البذور النابتة على وسط Murashige and Skoog الأساسي (MS) و الصلب الذي يحتوي التركيز نفسه من الملوحة . كان نمو الجذور أكثر تأثراً بالتركيز 200 ميلي مول Nacl مقارنة بنمو البادرات . نقلت هذه الأخيرة , بعد ذلك , إلى أصص تحوي تربة مغذية (تورب), و رويت بماء ملحي (التركيز نفسه الذي نمت عليه), و تمت أقلمتها في المختبر . حصلنا على نباتات بحالة خضرية جيدة, استمر نموها إلى مرحلة النضج خلال 12-10 أسبوعاً.
The objective of this study is to compare the effect of five different Nacl concentration (0,50,100,150,200 mM) on seeds germination and seedling growth of two cultivars of soybean (sb-44,sb-172). Seeds were germinated in petri dishes with sterile filter paper moistened with 10 ml of test solution at 25 c˚ in dark . The obtained results showed that germination percentage , length of root and hypocotyl were decreased with the increase of the Nacl concentration in the medium. The best values was from cultivar sb-44 . After seven days of incubation , significant differences were observed between control and treated seeds . Germinated seeds were subcultured on basal Murashige and Skoog medium solid at the same Nacl concentration . The roots growth was mor affected by 200 mM Nacl compared to seedling growth . Plantlets were transfered into pots with nutrient soil , irrigated with saline water and were adapted at laboratory . we obtained good plants grew to maturity in 10- 12 weeks .
Artificial intelligence review:
Research summary
تهدف هذه الدراسة إلى مقارنة تأثير خمسة تراكيز مختلفة من الملوحة (Nacl) (0، 50، 100، 150، 200 ميلي مول) على إنبات بذور ونمو بادرات صنفين من فول الصويا (sb-44 و sb-172). تم إنبات البذور في أطباق بتري على أوراق ترشيح معقمة ومرطبة بـ 10 مل من الوسط المستخدم عند درجة حرارة 25 م° وفي الظلام. أظهرت النتائج أن نسبة الإنبات وطول الجذور والسويقات تقل مع زيادة تركيز Nacl في الوسط، وكانت أفضل القيم من الصنف 44-sb. بعد سبعة أيام من الحضانة، لوحظت فروق معنوية بين الشاهد والبذور المعالجة. زرعت البذور النابتة على وسط Murashige and Skoog (MS) الصلب الذي يحتوي على نفس تركيز الملوحة. كان نمو الجذور أكثر تأثراً بتركيز 200 ميلي مول Nacl مقارنة بنمو البادرات. نقلت البادرات بعد ذلك إلى أصص تحتوي على تربة مغذية (تورب) ورويت بماء ملحي بنفس التركيز الذي نمت عليه، وتمت أقلمتها في المختبر. حصلت النباتات على حالة خضرية جيدة واستمر نموها إلى مرحلة النضج خلال 10-12 أسبوعاً.
Critical review
دراسة نقدية: الدراسة تقدم معلومات قيمة حول تأثير الملوحة على إنبات ونمو فول الصويا، ولكن هناك بعض النقاط التي يمكن تحسينها. أولاً، كان من الأفضل تضمين مزيد من الأصناف لتقديم صورة أشمل عن تأثير الملوحة. ثانياً، لم يتم توضيح تأثير الملوحة على المكونات الكيميائية للنباتات بشكل كافٍ. ثالثاً، كان من الممكن استخدام تقنيات تحليلية أكثر تطوراً لتحديد تأثير الملوحة على مستوى الجينات والبروتينات. وأخيراً، كان من الممكن تقديم توصيات أكثر تفصيلاً للمزارعين بناءً على النتائج.
Questions related to the research
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ما هو الهدف الرئيسي من الدراسة؟
الهدف الرئيسي من الدراسة هو مقارنة تأثير خمسة تراكيز مختلفة من الملوحة (Nacl) على إنبات بذور ونمو بادرات صنفين من فول الصويا.
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ما هي أفضل القيم التي تم الحصول عليها في الدراسة؟
أفضل القيم تم الحصول عليها من الصنف 44-sb حيث كانت نسبة الإنبات وطول الجذور والسويقات أفضل مقارنة بالصنف الآخر.
-
كيف تم تحضير الوسط المغذي الأساسي؟
تم تحضير الوسط المغذي الأساسي باستخدام Murashige and Skoog (MS) وأضيفت إليه التراكيز الملحية المذكورة، ثم ضبطت درجة الـpH على 5.7 وأضيف إليه الآغار آغار بتركيز 8 غرام/لتر.
-
ما هي التوصيات التي قدمتها الدراسة؟
التوصيات تشمل دراسة مقارنة لأصناف أخرى لمعرفة درجة تحملها للملوحة واعتماد الأصناف الأكثر تحملاً للملوحة في الزراعة.
References used
ALMANSO URI,M.; KINET,J.M and LUTT,S. Effect of salt and osmotic stresses on germination in durum wheat. Plant soil, 231, 2001, 243-254
AMIRJANI,M.R. Effect of salinity stress on growth, mineral composition, proline content, antioxidant enzymes of soybean. Amer.J. of plant physiology. 5,2010, 350- 360
ATAK,M..; KAYA, N.D.; CIKILI,Y. and CIFTCI, C.Y. Effect of Nacl on the germination, seedling growth and water uptake of triticale. Turk.J.Agric.for.30,2006,39-47
rate research
Read More
The objective of this study is to compare the effect of five different Nacl concentration (0,50,100,150,200 mM) on seeds germination and seedling growth of two cultivars of soybean (sb-44,sb-172).
Seeds were germinated in petri dishes with sterile
filter paper moistened with 10 ml of test solution at 25 c˚ in dark .
The obtained results showed that germination percentage , length of root and hypocotyl were decreased with the increase of the Nacl concentration in the medium. The best values was from cultivar sb-44 .
After seven days of incubation , significant differences were observed between control and treated seeds .
Germinated seeds were subcultured on basal Murashige and Skoog medium solid at the same Nacl concentration . The roots growth was mor affected by 200 mM Nacl compared to seedling growth .
Plantlets were transfered into pots with nutrient soil , irrigated with saline water and were adapted at laboratory . we obtained good plants grew to maturity in 10- 12 weeks .
The aime of this study was to isolate and identify the seed borne fungi of two
varieties of Soybean, Sb172 and Sb44, using blotter method and P.D.A plate method.
21 species belong 10 genus were determined, saprophyte and pathogen Fungi.
P.D.A plat
e method showed the highest number of fungi in comparison with the
other method, and the fungus Aspergillus was the most frequent genus with a ratio of
44.2% for the variety sb172 and 45.14% for the variety sb44, followed by the fungus
Cladosporium and then Fusarium, whereas the genus Colletotrichum showed the lowest
frequent ratio (0.85%) with the variety Sb172.
The percentages of seeds germination and infection were differed between the two
varieties, and the ratio seeds germination of variety sb172 were higher than the variety
sb44 with the both methods of culture, and were equal 94% with prim variety and 92%
with the second ones by blotter method, whereas the ratio were 52% and 34% for both
varieties respectively by P.D.A plate method. However, the ratio of infection were, in
general, lower with prim variety than the second ones by the both method.
The results showed the seeds which were highest ratio infection appeared low ratio
germination .
The present study was conducted at the First of May Station belongs to the
General Commission For Scientific Agricultural Research (GCSAR) at
Damascus during 2011 and 2012 growing seasons to estimate gene action,
heritability, genotypic and phenot
ypic coefficient variations (GCV, PCV) and
genetic advance for two crosses of soybean. The five population (P1, P2, F1, F2,
F3) of each cross were grown in a randomized complete block design (RCBD)
with three replications in order to evaluate oil and protein contents and seed
yield traits. Results of mean square analysis showed significant differences
(p<0.05) among mean values of the five populations for all studied traits
between the populations of each cross. The second cross achieved high broad
and narrow sense heritability (66%, 44%) for protein content, high value of
phenotypic and genotypic variations in seed yield in the second cross achieved
(18.03,17.30) and high value of expected genetic advance was obtained 11.09%.
In most traits, epistasis of the dominance × dominance type of gene action
occupied the first rank in order of importance in controlling inheritance of the
evaluate traits in both crosses indicating the importance of selection in late
segregating generations to improve these traits. These findings are supported
by high values of heritability, which was related with low to moderate expected
genetic advance in two crosses.
This investigation was conducted at the laboratories of Horticulture
Departments, Faculty of Agriculture to study the effect of different levels of
salinity (NaCl) in the rate and the percentage of seed germination of lettuce,
Lactuca sativa L. va
rieties: Shinshar and Kabous and to determine the seedling
response to these levels of salinity with relative to root length, the length of the
cold, fresh and dry weight of seedlings. NaCl was used at 0, 50, 100, 150 and
200 mM/l. Resulted showed that a gradual increase in salinity concentrations
caused a gradual decline in all indicators studied in both cultivars. However,
the plants of Kabous variety were less sensitive to the salinity and also higher
than in the indicators studied for seedlings (rate of germination and percent,
stems and roots length, fresh and dry weight of seedlings) under salt stress
conditions.
The effect of salinity and the treatment with fungicide on seed germination of Cupressus
sempervirens L. was investigated.
Five concentrations of salinity (0, 0.5, 1, 5 and 10%) were used. One part of the seeds was
treated with fungicides (Benomyl
), while the other part was germinated without fungicide
treatment. The results showed that seed germination was reduced by salinity, but the reduction was
more pronounced without fungicide and with salt concentration, the seeds didn't germinate at 5 and
10% NaCl.
Despite the decrease of germination indices with the increase of salt concentrations, the
statistical results didn't show significant differences in salinity concentrations and the control in
germination speed without fungicide, but they were significant with the fungicide. The statistical
results showed significant differences between the presence and absence of fungicide in all
treatments
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