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Identification and characterization of local isolates of lactic acid bacteria and testing their ability to produce diacetyl

تعريف و توصيف عزلات محلية من بكتيريا حمض اللبن و اختبار قدرتها على إنتاج ثنائي الأسيتيل

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 Publication date 2015
and research's language is العربية
 Created by Shamra Editor




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Fifty eight isolates of lactic acid bacteria were obtained from different local fermented foods. Isolates were identified using microscopic, cultural and biochemical tests using API strips. Results showed that 44.83% of the isolates were rod shaped and 55.17% belonged to cocci bacteria.



References used
Adebayo-tayo, B. C and A. A. Onilude. 2008. Screening of Lactic Acid Bacteria Strains Isolated from Some Nigerian Fermented Foods for EPS Production. World Applied Sci J. 4 (5): 741-747
Anunziata, O. A., L. B. Pierella and A. R. Beltramone. 2001. Kinetic studies on diacetyl synthesis over V-containing zeolites. Catalysis Letters, 75(1):87-91
Begovic, J., J. B. Brandsma. B. Jovcic. M. Tolinacki. K. Veljovic. W. C. Meijer and L. Topisirovic. 2011. Analysis of dominant lactic acid bacteria from artisanal raw milk cheeses produced on the mountanin stara planina, Serbia. Arch. Biol. Sci., Belgrade, 63 (1): 11-20
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Thirty strains of lactic acid bacteria were isolated from different local fermented foods. Isolates were identified by using morphological, cultural and biochemical tests using the API 50-CHL and API 20 Strep systems. Preliminary characterization tests showed that 33.33% of the isolates were rod shaped and 66.67% belonged to cocci bacteria.
This research was conducted at the Faculty of Agriculture, Department of Food Science laboratories to isolate lactic acid bacteria from some Syrian milk products (milk, yogurt, cheese) and determine some morphological, physiological characteristic and the ability of these isolates to ferment sugars.
Forty Isolates of Streptomyces bacteria were obtained from soil samples from different regions of Syria (Damascus, Sweida, Daraa, Homs, Latakia, Damascus, Hasakeh, Deir-ezzor) during 2012. The isolates were identified using ISP (International Stre ptomyces Project) methodology based on morphological and physiological criteria. Isolates were grown on selective solid media depending on growth density as an indicator of the consumption of xylose as an unique carbon source then on liquid media to determine their ability to produce the glucose isomerase enzyme. The crude enzyme was extracted using CTAB 0.1% solution, the enzyme activity was estimated using glucose as a substrate and the end product was measured colorimetrically at 560 nm. Results showed that the 40 isolates belong to 29 different species of Streptomyces. All isolates were able to produce glucose isomerase but they varied in their efficiency. Isolate SH10 (S. roseiscleroticus) was distinguished by its high level of enzyme production reaching 4.9 units /ml.
Total of 33 isolates of lactic acid bacteria from different samples (Dairy products, Pickles), were isolated and identified according to the common characteristics of lactic acid bacteria. Twinty-five isolates were rods and 9 were cocci.
Four strains of lactic acid bacteria (LAB) from different genus، species and sources were tested for their abilities to bind of AFM1 for 14 hours. Lactobacillus rhamnosus LC705 showed higher ability to bind AFM1 (88.35%)، followed by Bifidobacteri um bifidum BG1 (84.21%), Lactobacillus bulgaricus R21 by 80.41% and finally Streptococcus thermophilus by 71.52% after 14 hours of incubation.
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