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Register a new userMulti-Differentiation Potential for Mesenchymal Stem Cells Isolated from Human Umbilical Cord
كمون التمايز المتعدد لخلايا جذعية ميزنشيمية معزولة من الحبل السري البشري
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Damascus University ورقة بحثية
Publication date
2013
fields
Pharmacy
and research's language is
العربية
Authors
مجد الجمالي( باحث )
Created by
Shamra Editor
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تتمّتع الخلايا الجذعية بقدرةٍ فريدة على التمايز إلى العديد من أنواع الخلايا التي تعوض بشكل طبيعي الفاقد من بعض خلايا الجسم و اللاحق لإصابة نسيجية. و يعد دم الحبل السري و نسيجه المصدرين الأهم للحصول على الخلايا الجذعية الموّلدة للدم HSCs و الخلايا الجذعية الميزنشيمية MSCs , على الترتيب، و يشكلان المادة الرئيسة في بنوك الخلايا الجذعية التي أسست في معظم أرجاء العالم و حديثاً جداً في سورية. مع ذلك، فقد ركزت البحوث في منطقتنا بشكل رئيس على أساليب حفظ الخلايا و تجميدها مع القليل من الدراسات التي أُجريت لتحديد قدرة الخلايا المحفوظة على التمايز في الزجاج. هدف هذا البحث إلى اختبار القدرة الكامنة لخلايا جذعية ميزنشيمية، معزولة من نسيج حبل سري بشري مأخوذ من ولادة قيصرية في مشفى التوليد الجامعي في دمشق و محفوظة بالتجميد، على التمايز لأنواع مختلفة من الخلايا استجابةً لعوامل نمو و تحريض نوعية لبعض النسائل الخلوية.
Stem cells have unique capability to differentiate into many cell types that can normally replace the loss in some cells of the body due to tissue injury. Umbilical cord blood (UCB) and umbilical cord (UC) are the two main sources for hematopoietic stem cells (HSCs) and mesenchymal stem cells (MSCs), respectively, which constitutes the basis for stem cell banks that have been established worldwide and very recently in Syria. Research in our region has mainly focused on cell storage and freezing protocols, and only few studies were conducted to prove the ability of the stored cells to differentiate into their destined lineages. This study aimed to test the potential of cryopreserved MSCs isolated from an umbilical cord taken from new delivery at Maternity University Hospital in Damascus, to differentiate into various types of cells in response to growth and induction factors specific to cell lineages.
References used
Trounson A. (2009) New perspectives in human stem cell therapeutic research. BMC Medicine, 7(29): 1-5
Helmy KY, Patel SA, Silverio K, Pliner L, and Rameshwar P. (2010) Stem cells and regenerative medicine: accomplishments to date and future promise. The Deliv, 1(5): 693-705
Sensebe, L., Krampera, M., et al. (2010) Mesenchymal stem cells for clinical application. Vox Sang; 98: 93- 107
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This research aims at establishing
and testing protocols for isolation, in vitro proliferation, phenotyping, and
differentiation of MSCs embedded in umbilical cord tissues of Syrian
newborns.
MSCs were isolated from two caesarian births depending
on their
adherence characteristic on plastic surfaces, and cells were cultured in MSC
medium for their growth and proliferation. Cell phenotyping was performed by
flow cytometry using fluorescent monoclonal antibodies specific to MSCs’
surface markers. Cultured cells were passed several times and a portion of
these cells was cryopreserved in liquid nitrogen, and cell viability was assessed.
Differentiation of these MSCs into adipocytes was conducted using culture
medium Indomethacin and hydrocortisone.
The study included 143 children from 214 newborn with high umbilical cord blood IgE (UC – IgE) at birth: more than 0.5 international unit per ml. We followed up these children from birth for 8 years.
We found positive atopic familial history by 51.7
% from study samples, against negative history by 48.3% .
During control duration atopic diseases were developed by 76.22 % of children with high UC – IgE, against 23.8 % without atopic diseases.
These children with atopic different disorders suffered from allergic rhinitis 19.6 % , allergic skin disorders (eczema, urticaria) 25.2 % and asthma 31.5% .
The rate of atopic diseases development was 51.7% in children with high UC-IgE, and positive history together, while only 24.5 % in children with high UC-IgE with negative familial history.
40 samples were collected from milk from Homs to isolate the
bacteria and study their ability to produce the enzyme β-
galactosidase and diagnose isolates the most efficient in the
production of the enzyme, the results showed the presence of 25
sporeforming isolates producing the enzyme β-galactosidase , the
effectiveness of β-galactosidase was estimated using
spectrophotometer at a wavelength of 420 nm.
In this work, our goal is recognizing human action from video data. First we
propose an overview about Human Action Recognition, includes the famous
methods and previous algorithms, then we propose an algorithm and its
implementation using MATLAB.
The goal of this research is to study the expression of epidermal and
transforming growth factor receptor in normal human bone marrow (BM)
cell, and in leukemia ( myeloid and lymphatic leukemia ) which promoted
the research for the function of EGF
R receptors in the development and
growth of BM cells and for malignancies expressing of the EGFR as
potential therapeutic targets in blood cell malignant diseases.
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