40 samples were collected from milk from Homs to isolate the
bacteria and study their ability to produce the enzyme β-
galactosidase and diagnose isolates the most efficient in the
production of the enzyme, the results showed the presence of 25
sporeforming isolates producing the enzyme β-galactosidase , the
effectiveness of β-galactosidase was estimated using
spectrophotometer at a wavelength of 420 nm.
The conditions for producing cellulase enzyme were optimized using corn
husks as substrate in submerged fermentation. The effects of five parameters
(incubation temperature, pH, substrate concentration, inoculum volume,
fermentation time) on the s
train growth and enzyme production were studied.
For this purpose the statistical program Minitab and the statistical design
Response Surface Methodology (RSM) were applied.
A partial purification was performed for the fungal hydroxycinnamic acid
esterase (HCAE) from Humicola sp. by precipitation using either ammonium
sulfate or acetone.
Thirty randomized samples of sugar materials were collected from different
places (Tartous, Damascus, Homs) in Syria for isolation and identification of
some yeasts. These yeasts were classified physiologically by API technique
to identify the spe
cies of Yeasts found in these materials. Results showed that
the isolated materials contained 17 isolated yeasts distributed in 3 genus and 7
species and named as follows: One isolated yeast from Geotrichum capitatum,
Candida krusei,Candida guilliermondi, Candida fumata and Candida zeylanoides
and 4 isolated yeasts from Candida lusitaniae and 7 isolated yeasts from
Saccharomyces cerevisiae. The classified yeasts were screened to investigate the
activity of internal and external invertase produced by them using
spectrophotometer at 540 nm. The results showed that there were differences in
the ability of yeasts to produce invertase enzyme. Candida Lusitaniae-2 and
Saccharomyces cerevisiae-4 were found the best yeasts for production of
external invertase, whereas Candida Lusitaniae1 was the best for internal
invertase.
Specimens of serum from ١٦٨ healthy subjects (٨٠ men and ٨٨ women, ages
١٨ to ٧٨ years)were divided into three groups: fresh, storage at ٤°C, and
freezing at-٢٠°C .
Periodic essays for the activity of Butrylcholinesterase (BChE,EC. ٣٫١٫١٫٨)
were
made on each frozen and unfrozen specimen. Serum frozen at-٢٠°C and
left at ٤°C gave results similar to those from fresh specimens. Comparison of
samples from men and women revealed significantly higher activity in men
than in women (P<٠٫٠٠١). Activity was greatest in men aged ١٨-٥٠ years and
then declined to slightly lower values. In women the activity was less in those
under ٥٠ years old than in those above ٥٠.
