The Caper (Capparis spinosa L.) is Plant, which has an economic value and high medical efficiency. The populations of this plant have an evident morphological diversity in our nature.
This research is devoted for studying the diversity of the variet
ies of the plant Capparis spinosa L., depending on protein contents properties of leaves and seeds, by Quantitative study and Electrophoresis (SDS-PAGE) method.
The aim is to check if these norms confirm or change the Classification grade of the varieties of the studied plant.
Our results show that existence of homogeneity between these Varieties, in the same time an acceptable differences. This can be considered among classification standard to help distinguish the different forms of Capparis spinosa L. plant and still regarded as Varieties.
Research was carried out in (24) locations where two subspecies Capparis spinosa
were recorded in Syria . The subspecie C. spinosa subsp. rupestris was found in seven
locations, mostly in the southern region of the environment, while he found the S
yrian C.
spinosa subsp. spinosa in seventeen of the study sites in Syria. Chemical analysis showed
that, the soils of all location were not salty and pH ranged between (6.9-8.4), Soils texture
was clayey and clay loam in most locations. According dependant on equation of
Emberger, it was found that distribution of subspecies Capparis spinosa were located in a
wide range from bioclimatic very dry to semi- humid zone, through with dry, semi-dry,
and Hyper humid zones.
Samples of Capparis spinosa plant were collected from 6 sites in Aleppo and Lattakia
provinces. The genetic variations were studied using the AFLP technique in order to determine the
genotypes of the studied types using 3 primers, which showed ampl
ification. Statistical analyses
were conducted using the dissimilarity coefficient and genetic diversity coefficient. The A.C.P. and
the statistical -F were calculated, and the similarity dendrogram was constructed. The results
showed the following:
The presence of a certain number of specific alleles (descriminates) for each province.
The presence of genetic and reproductive isolation deterrent to gene flow between the two
provinces.
The heterozygoty average was a little higher in Lattakia (0.486) than in Aleppo (0.481). The
mean of genetic diversity coefficient of primers and individuals was a little higher in Aleppo
(0.677) than in Lattakia (0.653). The population mean was very close: (0.759) in Aleppo and
(0.760) in Lattakia. This may be explained on the basis of silent and specific alleles due to deletion
mutations, different selective effects as well as the reproduction system in the two cities.
The greatest genetic distance in Aleppo was (0.381) noted between (Al Dahea and Al Shekh
saaed), and in Lattakia (0.38) noted between (Al Amroniah and Jabla). However, the greatest
genetic similarity in Aleppo was (0.637) noted between (Al Shekh Saaed and Turkman Bareh), and
in Lattakia (0.675) noted between (Wata Deirzenon and Jabla). The variation between these values
was graduated.
The study of statistical –F showed the effect of the similarity factor in some populations.
This was more significant in Aleppo than in Lattakia, which indicates that the reproduction system
is more closed in Aleppo, and this refers as well to inbreeding or self pollination which showed
high genetic variations in these populations when compared with Lattakia.
The results of this study helped in determining primers that can be used as molecular
markers in a breeding program for Capparis spinosa as a medicinal plant. This technique showed
high efficiency in studying the similarity relationships between these two cities.
In the present study¸ twelve parameters of Capparis spinosa were studied which are: plant length¸
number of main branches¸ number of leaf doubles, leaf length, leaf width, leaf area¸ number of budes,
number of flowers, number of fruits, weight of t
he fruits, number of seeds, weight of seeds. Statistical
analysis have been done using mean¸ variation¸ A.F.C.
Ecological and morphological characterization and geographical distribution of caper plant, Capparis spinosa L. samples collected from various parts of Syria were investigated during 2010-2014. The antioxidant contents of caper samples were analyzed and the genetic diversity and genetic relationship among the samples were determined using the ISSR technique
