Self-medication with antibiotics is a worldwide problem and led to several problems such as antimicrobial resistance. A descriptive cross-sectional questionnaire-based study was conducted in Latakia city. Among 259 participants, 204(78.8%) reported a
ntibiotics self-medication within past one year. Respiratory problems such as common cold were the main indication for self-medication with antibiotics (62.9%) and amoxicillin+ clavulanic acid was the most antibiotic commonly used (35.37%). The results of this study confirm that self-medication with antibiotics is a frequent problem in Latakia city.
Tylosin and Spiramycin are medium-spectrum macrolide antibiotic used exclusively in veterinary medicine for the treatment of a wide range of infections.This research deals with the determination of optimal conditions for simultaneous separation and d
etermination of two macrolides antibiotics (Tylosin and Spyramicine), using C8 and C18 Chromatographic separation columns and doing the comparison between them in order to develop a rapid and sensitive method which can be used to measure these two compounds using High Performance Liquid Chromatography – Diode array detector (HPLC-DAD).
This study has used the gradient elution for mobile phase and revealed that the best conditions for separation and determination are conjugated with the best retention times and best areas for both studied compounds using a mobile phase consisted of an aqueous solution of anhydrated disodium Hydrogen Phosphate at pH=2.4 and an organic solution of acetonitrile with a ratio of 80:20v/v (solution A) and acetonitrile (solution B) [Na2HPO4(0.04M) pH:2.4/CAN (80:20v/v)]/ ACN, temperature 40°C for both columns, flow ratio of 1ml/min. for the mobile phase and maximum absorption wave length 280 nm, 232nm for Tylosin and Spyramicine respectively. The best peak areas are recorded as 5.759, 5.927 for Tylosin and Spyramicine 0.10ppm respectively, using C8 Chromatographic separation column in comparison with the best peak areas 4.432, 4.212 respectively at the same concentration using C18 Chromatographic separation column. It was noticed that the best retention times for Tylosin and Spyramicine were 7.013, 4.214min. respectively at concentration of 0.10ppm using C8 Chromatographic separation column in comparison with the best retention times 7.641, 5.898min. respectively at the same concentration using C18 Chromatographic separation column. The calibration curves for both separated compounds on C8 Chromatographic separation column showed a good linearity within a concentration range of 0.0010-0.10 ppm ≈ 1-100ppb at the two wave lengths λmax. = 280, 232nm respectively.
A total of 30 water samples and 45 infected fish (carp, Cyprinus carpio) were
collected from freshwater fish farm (Dam of 16 Tishreen-Lattakia) and
analysed bacteriological. Macroscopic examinations of infected fish had showed
the presence of haem
orrhagic skin lesions with brown or red spots throughout
their skin. A total of 64 Aeromonas strains were isolated. The Aeromonas
isolates were distributed as follows: Aeromonas hydrophila (34, 53%), A. caviae
(16, 25%), A. sobria (9, 14%) and (5, 8%) of unidentified aeromonads.
Collectively, Aeromonas spp. are considered as opportunistic causative agents
of human gastroenteritis and other infections. Antibiotic susceptibility tests
were carried on all strains of isolated Aeromonas spp. using twenty different
antibiotics by agar disk diffusion method.
A total of 294 samples of raw-unpasteurized bovine milk and variety of
dairy products (hard cheese, sweet cheese, cream cheese and cream), were
collected from local markets in Lattakia city as well as 44 samples of used water
(clear potable water
collected before used in cheese preparing processes) and
preserving water (turbid water collected from hard cheese preserving tanks).
All samples were analyzed for E.coli O157: H7 detection.
We screening for antimicrobial substances production from marine algae of
Syria. We presented results of ١٦ algal species tested by their lipide and
aqueous extracts agaimst Gram’s negative and positive bacteria, as well as one
fungous species.
T
he lipide not aqueous extracts were the active against Gram’s positive
bacteria especially.
No effect founded on the fongous species. In addition we make comparative
viewing with other studies on other mediterranean contries regarding the same
species of algae tested.
