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A total of 30 water samples and 45 infected fish (carp, Cyprinus carpio) were collected from freshwater fish farm (Dam of 16 Tishreen-Lattakia) and analysed bacteriological. Macroscopic examinations of infected fish had showed the presence of haem orrhagic skin lesions with brown or red spots throughout their skin. A total of 64 Aeromonas strains were isolated. The Aeromonas isolates were distributed as follows: Aeromonas hydrophila (34, 53%), A. caviae (16, 25%), A. sobria (9, 14%) and (5, 8%) of unidentified aeromonads. Collectively, Aeromonas spp. are considered as opportunistic causative agents of human gastroenteritis and other infections. Antibiotic susceptibility tests were carried on all strains of isolated Aeromonas spp. using twenty different antibiotics by agar disk diffusion method.
20 antibiotics were monitored for their sales for six months, through official prescriptions in six drug stores throughout Lattakia. Percent proportions of sale for each antibiotic were reported. These statistical data were correlated with laborat ory studies on screening these antibiotics on different bacterial strains that collected from different laboratories and hospitals of Lattakia. The purpose of this continuous study is to undertake an assessment of levels of antibiotic resistance by local isolated bacteria, and to determine the factors influencing the offspring of that resistance. 1430 medical prescriptions were reported, 965 of them (i.e.: 67, 48%) contained one or more antibiotic. In addition, there were 340 cases of nonofficial sales of some distinct antibiotics. 275 of bacterial strains were isolated from clinical specimens (mostly were as urogenital, pharyngeal, wounds, and burns infections). These strains distributed as follow: 183 (i.e.: 66, 54%), were Enterobacteriaceae strains.
A total of 370 bottles of noncarbonated natural mineral water (domestic and imported) with different bottling dates, were collected directly from the markets in Syria one word one year 2006. Bacterial diversity was approached with tentative identification of the strains isolated using biochemical and enzymatic criteria (with the aid of API 20 NE identification system for nonfermenters).
A total of 294 samples of raw-unpasteurized bovine milk and variety of dairy products (hard cheese, sweet cheese, cream cheese and cream), were collected from local markets in Lattakia city as well as 44 samples of used water (clear potable water collected before used in cheese preparing processes) and preserving water (turbid water collected from hard cheese preserving tanks). All samples were analyzed for E.coli O157: H7 detection.
A total of 73 drinking water samples were collected from distribution system in Lattakia city, the aim was to search for hetrotrophic bacteria and investigate their resistance to chlorine and antibiotics. The antibiotic susceptibility tests were: carried out by using agar dillution method (MIC) and the antibiotics tested were: Ampicillin, Chloramphenicol, tetracycline, Amikacin, Ciprofloxacin, Cefotaxim, Gentamicin, Erythromycin Piperacilin/ Tazobactam, and cephalothin. A sum of 133 bacterial strains were isolated and included by five genera: pseudomonas, acinetobacter, flavobacter, chromobacter, and methylobacter. The samples content of residual chlorine were ranged between 0.1 to 3.8 mg/l. The majority of bacterial strains isolated were resistance to chlorine. The multiple antibiotic resistances were common amongst isolated bacterial strains.
Due to the economical and medical importance of the Melilotus plant and the wide distribution of this plant in the Syria environment, it was of necessity to study the the composition of organic matter extracted from the leaves of this plant. The M elilotus Officinal organic matter was extracted and purified their where were studied by GC-Maas spectrometry. The organic matter was obtained by Soxhlet extractor and by solvent n-Hexane. Components have been found which was about (99.91%) from the total of Essential Oil. The major components were extracted by Soxhlet extractor and the following components were determined : n-Docosane(39.82%) , Hydrocoumarin(15.39%) , Methyl 3-(2-hydroxyphenyl)propionate(14.29%). The major components were determined by solvent n-Hexane: 1,2-Benzenedicarboxylic acid(17.77%) , 9,12,15-octadecatrienoic acid(12.85%) The effects of the extracted aromatic oil from the leaves of Melilotus plant on some of Pathogenic Microorganisms were studied.
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