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Isolation and diagnosis of bacteria causing urinary tract infection in humans and studying their sensitivity to antibiotics

عزل و تشخيص الجراثيم المسببة لالتهاب المجاري البولية عند الانسان و دراسة حساسيتها تجاه الصادات الحيوية

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 Publication date 2017
and research's language is العربية
 Created by Shamra Editor




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Bacterial pathogens associated with urinary tract infection were studied. The study examined 120 urine samples taken from the middle of the urinary flow of patients with symptoms of urinary tract infection.


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Research summary
تتناول هذه الدراسة تحليل الجراثيم المسببة لالتهاب المجاري البولية لدى الإنسان، بالإضافة إلى دراسة حساسيتها تجاه المضادات الحيوية. تم جمع 120 عينة بول من مرضى يعانون من أعراض التهاب المجاري البولية في مستشفى الأمين بحمص وعيادات خارجية بين يوليو وأكتوبر 2016. أظهرت النتائج أن 100 عينة كانت موجبة للزرع الجرثومي، مع نسبة إصابة أعلى بين الإناث مقارنة بالذكور بنسبة 3:1. كما أظهرت الدراسة أن 28% من المصابين كانوا يعانون من مرض السكري، وأن 44% من النساء المصابات كن حوامل. تم تحديد أنواع الجراثيم باستخدام أوساط زرعية واختبارات كيميائية حيوية، حيث كانت E.coli هي الأكثر شيوعاً بنسبة 60%، تليها Staphylococcus وKlebsiella بنسبة 16% لكل منهما، ثم Enterobacter وPseudomonas بنسبة 8% لكل منهما. أظهرت النتائج تبايناً في استجابة الجراثيم للمضادات الحيوية، حيث أبدت E.coli مقاومة عالية تجاه CTX وCRO بنسبة 67%، بينما كانت حساسة بنسبة 73% تجاه AMK. أظهرت Enterobacter مقاومة بنسبة 38% تجاه CTX وCRO، وحساسية بنسبة 100% تجاه AMK وF. أبدت Klebsiella مقاومة بنسبة 50% تجاه CIP، وحساسية بنسبة 75% تجاه AMK وNOR وF. أظهرت Staphylococcus مقاومة بنسبة 50% تجاه AMK، وحساسية بنسبة 100% تجاه CIP وCTX وCRP. أظهرت Pseudomonas مقاومة بنسبة 100% تجاه CTX وCRP، وحساسية بنسبة 75% تجاه NOR وGN. توصي الدراسة بضرورة إجراء اختبار الحساسية للمضادات الحيوية قبل التداوي بها لتقليل ظهور سلالات مقاومة.
Critical review
تعتبر هذه الدراسة قيمة في تسليط الضوء على الجراثيم المسببة لالتهاب المجاري البولية وحساسيتها تجاه المضادات الحيوية. ومع ذلك، يمكن تحسين الدراسة من خلال توسيع نطاق العينة لتشمل مناطق جغرافية مختلفة لزيادة تنوع البيانات. كما يمكن استخدام تقنيات حديثة مثل تحليل الجينوم الكامل للجراثيم لفهم أفضل لآليات المقاومة. بالإضافة إلى ذلك، يمكن تضمين دراسة تأثير العوامل البيئية والصحية الأخرى على انتشار الجراثيم ومقاومتها للمضادات الحيوية.
Questions related to the research
  1. ما هي الجرثومة الأكثر شيوعاً في التسبب بالتهاب المجاري البولية حسب الدراسة؟

    الجرثومة الأكثر شيوعاً هي E.coli بنسبة 60%.

  2. ما نسبة الإصابة بالتهاب المجاري البولية بين الإناث مقارنة بالذكور؟

    نسبة الإصابة بين الإناث مقارنة بالذكور هي 3:1.

  3. ما هي نسبة مقاومة E.coli تجاه المضاد الحيوي CTX؟

    نسبة مقاومة E.coli تجاه المضاد الحيوي CTX هي 67%.

  4. ما هي التوصيات الرئيسية التي قدمتها الدراسة؟

    توصي الدراسة بضرورة إجراء اختبار الحساسية للمضادات الحيوية قبل التداوي بها لتقليل ظهور سلالات مقاومة.


References used
Foxman, B. 2002. Epidemiology of urinary tract infections: incidence, morbidity, and economic costs. Am. J. Med. 113(Suppl. -1A):5S–13S
Hooton, T. M., and W. E. Stamm. 1997. Diagnosis and treatment of uncomplicated urinary tract infection. Infect. Dis. Clin. N. Am. 11:551–581
Schappert SM, Rechtsteiner EA (2011) Ambulatory medical care utilization estimates for 2007. Vital Health Stat 13: 1–38
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Objective: Evaluate sensitivity and specificity of nitrite test to urine culture(gold standard) for diagnosing urinary tract infection in patients with sickle cell anemia with urinary symptoms and signs. Methods: The study sample included 79 chi ldren aged 2 to 14 years with SCA presenting with fever (temperature >38°C),abdominal pain, urgency, dysuria or increased urinary frequency who referral to the general pediatric clinic and the children's ambulance department in Tishreen University Hospital in Lattakia during( 2016-2017). Midstream urine specimen was collected from children. Samples were subjected to nitrite test, culture and sensitivity. Results : There was significant bacteriuria in 17 (21.5%) children with SCA. A positive test for nitrite was obtained in 12 of the 17(70.5%) children. The nitrite test has a specificity of 95.2% in detecting bacteriuria, a sensitivity of 70.6%, a positive predictive value of 80% and a negative predictive value of 92%. A positive nitrite test was significantly associated with bacteriuria, while a negative test was also significantly associated with an absence of bacteriuria.
204 samples were collected from various body samples during the year 2016. These 204 samples distributed as follows: 133 from urine , 37 swabs of wounds and burns, 10 swabs from the ear and pharynx, 12 from feces, 8 from CSF, 4 from blood. This s tudy distinctly showed that gram negative bacilli were the most isolates 136 (67%) while 68 isolates (33%) were gram positive bacteria . E. coli dominated (39.68%) of the total gram negative bacilli. This study distinctly showed an increase in the ratio of E. coli resistance to one or more types of antibiotics , we found that E. coli resistance to all antibiotics except specific and expensive antibiotics, and we found that its complete resistance to the second ,third and fourth generation of cephalosporins , resistance to specific and antibiotics such as Imipenem.
A total of 73 drinking water samples were collected from distribution system in Lattakia city, the aim was to search for hetrotrophic bacteria and investigate their resistance to chlorine and antibiotics. The antibiotic susceptibility tests were: carried out by using agar dillution method (MIC) and the antibiotics tested were: Ampicillin, Chloramphenicol, tetracycline, Amikacin, Ciprofloxacin, Cefotaxim, Gentamicin, Erythromycin Piperacilin/ Tazobactam, and cephalothin. A sum of 133 bacterial strains were isolated and included by five genera: pseudomonas, acinetobacter, flavobacter, chromobacter, and methylobacter. The samples content of residual chlorine were ranged between 0.1 to 3.8 mg/l. The majority of bacterial strains isolated were resistance to chlorine. The multiple antibiotic resistances were common amongst isolated bacterial strains.
Introduction: Urinary tract infections are very common in childhood (especially under the age of 5 years) and because of the large number of complications resulting from it (renal failure – hypertension- kidney scarring) had to be diagnosed and early treatment, although that urine culture is the standard Golden diagnosis, but its results are late to appear, Many studies have indicated an accompanying increase in platelet counts in urinary tract infections, so that platelet counts may be a vital indicator directed to it without asylum to the costly analyzes in its diagnosis. Objective: the objective of the following study is to assess the sensitivity and specificity of the plate count (PLT) in UTI. Methods: The study included 65 Patients (14 males, 51females) They were between (3-6) year with upper urine tract infections were diagnosed through symptoms, urological test, Serum tests, and radiology, who were admitted to the pediatric department at Tishreen University Hospital in Lattakia City. PLT was obtained through a complete CBC blood count (sensitivity and sensitization), and comparison of the ratio Percentage of Thrombocytosis between urine culture with Gram-positive germs and Gram-negative germs. Results: The study showed a good sensitivity=80.39% [75–84] to platelet counts compared to urine culture. it also showed statistically significant paper p-value=0.003 between platelet count and type of pathogen (positive or negative Gram) and the increase was more favorable to positive urine culture. Also showed that the average value of platelets is higher in culture a positive gram 521.9±90.9 x103 platelet/mcl than in gram negative 432.3±73.5 x103 platelet/mcl. Conclusion: higher Platelet numbers are a good analysis or sensitivity for the diagnosis of upper urinary tract infection in children with reliable urinary symptoms to start treatment. So that we get the results of culture urine .and the higher platelet count must be taken gram positive bacteria into account.
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