نفــذ البحـــث لتقييـــــم الفعاليـة التثبيطيـة لمستخـص الأسيتـون، الإيتانـــــــول، الميتانــــــــــــول و الكلوروفـــــــــــورم لأشنـة Evernia prunastri و بتراكيز (25،50،75،100) ملغ/مل تجاه ثلاثة أنواع ممرضة من جنس Aspergillus هي: A. niger , , A. fumigatus A. flavus . تباينت فاعلية المستخلصات الأربعة، حيث أبدى كلا المستخلصين الأسيتوني و الميتانولي فعاليـــــــــة تثبيطيـــــــــــة ملحوظــــــــة تجاه الانواع الفطريــــــــــــة المدروســـــــــــة، إذ تزايـــــــدت الفاعليـــــــــة بازديـــــــــاد تراكيزهمـــــا، فعنــــــــــد التركــــــــــــيز (100)ملغ/مل ثبــــــــــــط النـــمـــــــــــو كلّياً لكـــــــــــــــلّ مــــــــــــــن A. niger , , A. fumigatus A. flavus ، بينما وصلت نسبـة التثبيــط لمستخلـص الإيتانول إلى (94.11، 78.82، 87.05)% على التوالي عند التركيز ذاته، أما بالنسبة لمستخلص الكلوروفورم فكان الأقل تأثيراً، حتّى عند التركيز (100) ملغ/مل، حيث بلغت نسبة التثبيــــــــــط 62.35) ،74.11، 68.23) % على التوالي مقارنة بالشاهد A. fumigatus.
اعتماداً على هذه النتائج يمكن الاستفادة من مستخلصات أشنة Evernia prunastri كمصادر حيوية طبيعية لمعالجة الإنتانات الفطرية مستقبلاً.
The research was carried out to evaluate the inhibitory efficacy of acetone, ethanol,
and chloroform of Evernia prunastri lichen with concentrations of (25, 50, 75, 100) mg/ml.
against three pathogenic species of Aspergillus sp. Which are: A. flavus, A. fumigatus and
A. niger . The efficacy varied among the four extracts, where both the acetonic and
methanolic showed noticeable inhibitory efficacy against the fungal species under study.
The efficacy increased of both extracts, at concentration (100) mg/ml growth of A. flavus,
A. fumigatus and A. niger was completely inhibited, whereas the inhibiton percentage for
ethanol extract reached (87.05, 78.82, 94.11)%, respectively at the same concentration. As
for the chloroform extract, it was the least effective, where the percentage at (100) mg/ml
concentration reached (68.23, 74.11, 62.35) %, respectively, compared to the control.
Depending on these results, the extracts lichen of Evernia prunastri could be used
as natural products to treat fungal infections in the future.
References used
AMITANI, R.; MURAYAMA, T.; NAWADA, R.; LEE, W.J.; NIIMI, A.; SUZUKI, K.; TANAKA, E. AND KUZE, F: Aspergillus culture filtrates and sputum sols from patients with pulmonary aspergillosis cause damage to human respiratory ciliated epithelium in vitro. European Respiratory Journal, Japan, Vol. 1, N .8, 1995, 1681-1687
BABIAH, P.S; UPRETI, D.K; JOHN, SA . Assessment of fungicidal potential of lichen Heterodermia leucomelos (L.) Poelt against pathogenic fungi. Current Research in Environmental & Applied Mycology, Vol. 5, N. 2, 2015, 92–100
(BABIAH, P. S.; UPRETI, D.K. AND JOHN S.A. An in vitro analysis of antifungal potential of lichen species Parmotrema reticulatum against phytopathogenic fungi. International journal of current microbiology and applied sciences, India, Vol .3, N ,12, 2014, 511-518. (a
This study was conducted to determine the effect of lichen Evernia
prunastri extracts (acetone, ethanol, and chloroform) against the two
species of plant pathogenic fungi were Fusarium oxysporum, F.solani.
All extracts showed has inhibition influence evident in the growth of
fungal and germination of spores of two compared to the control.
This study was carried out to evaluate the inhibitory effect of aqueous, ethanolic and
methanolic extracts of the cladodes, flowers and fruits cactus plant (Opuntia ficus-indica)
with concentrations of (125, 250, 500, 1000) mg/ml against the isolat
In this research was studied the effect of different concentrations of (ethanolic –
methanolic – acetonic) extracts of Apium nodiflorum on growth of fusarium moniliforme
all extracts revealed clear inhibitory effect against this Fungi.
The inhibit
The antibacterial activity of Inula viscosa L. leaves extracts against some pathogenic bacteria, which were isolated from Al-Assad hospital laboratory in Lattakia, was tested by disc diffusion method.
Results showed that water extracts had antibacte
Four isolates of Streptomyces sp., St.1 and St.2 were isolated
from garden soil of science faculty, St.3 and St.4 were isolated
from Al-Shameia soil Lattakia, by serial dilution on Starch Casein
Agar medium.