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Register a new userExtract of pyocyanin from marine Pseudomonas aeruginosa and its antibacterial effect against some pathogenic bacteria
استخلاص البيوسيانين من جراثيم الزائفة الزنجارية Pseudomonas aeruginosa البحرية و تأثيره الصاد في بعض الجراثيم الممرضة
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Aِl-Baath University ورقة بحثية
Publication date
2017
fields
Biology
and research's language is
العربية
Authors
أسمهان زينب( باحث )
Created by
Shamra Editor
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تّم استخلاص البيوسيانين من مزارع جراثيم الزائفة الزنجارية المعزولة من المياه البحرية بالمذيب العضوي الكلوروفورم. أظهرت نتائج دراسة تأثير البيوسيانين في الجراثيم الممرضة المعزولة من عينات مرضية مأخوذة من مختبر مستشفى الأسد الجامعي في اللاذقية بطريقة الانتشار بوساطة الأقراص أن البيوسيانين يمتلك تأثيرًا صاداً تجاه الجراثيم الإيجابية صبغة غرام المختبرة.
Pyocyanin was extracted from cultures of Pseudomonas aeruginosa, which was isolated from sea water by chloroform. Results study the effect of pyocyanin against human pathogenic bacteria, which were isolated from Al-Assad hospital laboratory in Lattakia, with disk diffusion method showed that pyocyanin has antibacterial activity against Gram- positive bacteria.
References used
AGRAWAL, H. A. and CHAUHAN, B. P., 2016. Effect of Cultivation Media Component in Antimicrobial Property. International Journal of Current Advanced Research. Vol. 5, 829- 833
BARKER, G. A.; KEHOE, E., 1995. Assessment of disc diffusion methods for susceptibility testing of Aeromonas salmonicida. Aquaculture. Vol. 134, P. 1-8
BISWAS, K.; PAUL, D.; SINHA, N. S., 2016. Marine Bacteria: A Potential Tool for Antibacterial Activity. Journal of Applied & Environmental Microbiology. Vol. 4 (1), 25-29
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The antibacterial activity of Inula viscosa L. leaves extracts against some pathogenic bacteria, which were isolated from Al-Assad hospital laboratory in Lattakia, was tested by disc diffusion method.
Results showed that water extracts had antibacte
rial activity against all Gram positive and negative bacteria. The diameters of inhibition zones were between 9 mm against Pseudomonas aeroginosa and 41 mm against Proteus vulgaris.
All Gram positive bacteria showed sensitivity to all organic extracts, so they had antibacterial activity, while Gram negative bacteria showed resistance to chloroform extract except for the one of Klebsiella pneumoniae with an inhibition zone of 12 mm, and resistance for dichlormethan extract except for Pseudomonas aeruginosa with an inhibition zone of 15 mm. there was resistance for diethyl extract except for Serratia marcescens with an inhibition zone of 32 mm.
Ethyl acetate extract had antibacterial activity against all bacteria. Ethanol, methanol and acetone extracts showed antibacterial activity against all bacteria except for Escherichia coli with an inhibition zone of 10 mm.
This study indicated that water and organic Inula viscosa L. leaves extracts had antibacterial activity against pathogenic bacteria which also was tested, especially water extract, ethanol, methanol, ethyl acetate and hexane extracts. So the Inula viscosa L. leaves extracts may be sources for natural antibiotic in future.
Four isolates of Streptomyces sp., St.1 and St.2 were isolated
from garden soil of science faculty, St.3 and St.4 were isolated
from Al-Shameia soil Lattakia, by serial dilution on Starch Casein
Agar medium.
Inhibition activity of extracts (acetone, ethanol, water) parts of the (Myrtus
communis L.) (root, stem, leaves, fruits) with concentrations (25,50,75,100%)
was carried out against growth of Gr- and Gr+ bacteria which were isolated and
identified
from the pathogenic specimens of The University Children's Hospital
bacterial laboratory in Damascus, using agar well diffusion method on Mueller
– Hinton medium.
Isolation and identification of Pseudomonas aeruginosa was screened from
15 samples of Syrian oily-soils to determine the ability to produce Lipase
enzyme by Cetermide Agar, Tributrin Agar and Brain Heart Broth during
2007-2008 AD. The results sho
wed that there were grown colonies with color of
green or yellow-green surrounded with different diameter of clear zone in three
samples and the identification by Mowasat Hospital revealed that it was
Pseudomonas aeruginosa In addition, the results showed that the optimum
conditions to produce lipase was 35 Cº with the pH degree of 8 and the time of
production of lipase with highest activity of 63.3% was three days in
submerged culture during the optimum conditions. These results illustrated the
important of this isolates to produce and control lipase for industrial
production.
Activity of Ziziphora tenuior L. leaves organic extracts against four pathogenic
bacteria, which were isolated from clinical samples and prepared from Al-Assad hospital
laboratory in Lattakia, were tested by disc diffusion method through 2014.
Res
ults showed that Ziziphora tenuior L. leaves extracts have antibacterial activity
against all Gram positive and negative bacteria. Inhibition zones were recorded between
9mm against Pseudomonas aeruginosa and 44mm against Staphylococcus aureus at
concentration of 200mg/ml for the methanolic extract. Whereas the inhibition zones for the
ethanolic extract were recorded between 7mm at concentration of 40mg/ml against
Streptococcus sp. and 34mm at concentration of 200mg/ml against Staphylococcus aureus.
These results represent the importance of Ziziphora tenuior L. as a source of natural
substances counteraction of pathogenic bacteria.
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