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Register a new userLeishmania major common in Damascus
الليشمانية الجلدية التي يسببها النوع L. major و المنتشرة في ريف دمشق
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محمود قويدر( باحث )
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عزلت عدة سلالات من الليشمانية الجلدية من أشخاص مصابين بهذا الطفيلي، في منطقة ريف دمشق (الرحيبة)، و جرى استنباتها في الزجاج على أوساط نصف صلبة (آغار +دم أرنب) في المرحلة الأولى ثم على أوساط سائلة وحيدة الطور المدعمة بالمصل البقري الحيوي المجرد من المتممة؛ و حددت الشروط النموذجية للاستنبات. كما تم تحديد نمط هذه الطفيليات على أنها من نوع الليشمانية الجلدي و ذلك باستعمال تقانة الرحلان الكهربائي، متماثل الأنزيمات.
Several strains of leishmania were isolated from patients suffering from cutaneous leishmaniasis in Damascus countryside; and cultured in vitro on semisolid medium (agar with rabbits blood) at first, then in monophasic RPMI-١٦٤٠ medium supplemented with ١٠٪ (v/v) heatinactivated fetal calf serum to determine the ideal conditions of culture. The types of these strains were specified as Leishmania major using isoenzymatic electrophoresis.
References used
ADLER,S. (١٩٦٤) .Leishmania .Advances in Parasitology
BLACKWELL, J.M.,R.A.B. EZEKOWITZ, M. B. ROBERTS, J. Y. CHANNON, R.B.SIM,and S.GORDON.(١٩٨٥).Macrophage complement and lectin-like receptors bind Leishmania in the absence of serum. J.Exp
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In this study the total of ٤١٥ cases of cutaneous leishmaniasis was evaluated
from Dermatology hospital and General clinic in Damascus from February
٢٠٠١ to February ٢٠٠٢. Most of these cases was reported in Damascus and its
suburb, and when we ma
de the direct microscopic test, amastigotes were
detectable in ٢٧١ cases, and the positive cases was increased to ٣٦٤ cases by
realizing the parasite culture into diphasic N. N. N. media, which confirm the
diagnosis.
That study was based on a BLAST search analysis performed against the
complete genome sequences using as a query sequence some of the PSA protein
sequences reported in the literature. In our study, we have first isolated,
cultivated, typed and str
ain of L. major. Then we have investigated
experimentally the complete list of PSA genes coding for membrane proteins in
its genome, using PCR approach based on specific primers. In conclusion, we
have detected the physical presence of 22 different genes coding for membrane
proteins in the genome of Syrian strain of L. major.
In our study, the
presence and expression of P27 gene in L.tropica evaluated in
promastigotes by PCR and RT-PCR using specific primer pairs
that manually designed after determine the consensus sequence of
forward and reverse region of primers bet
ween species of
Leishmania by using MUSCLE (Multiple Sequence Comparison by
Log- Expectation) tool for alignment. The results proved the
presence of P27 gene in Leishmania tropica, beside presence the
mRNA of P27 gene that confirm that P27 expressed in
promastigote form of L.tropica
Tinea capitis (TC) is a common superficial fungal infection of the scalp caused
by dermatophytes, mainly by the genera Trichophyton and Microsporum.It is primarily a disease of prepubertal
children, being more common in males than females . The eto
logical factors vary from one
region to another and can change within a particular region over time.
This study aim to identify the The most common fungal species which are responsible for producing TC in
children seen in Dermatology and Veneral Diseases Hospital in Damascus.
The research aims at identifying the level of the reality of the
special services provided by the institutes of care for the visually
impaired from the perspective of parents in the governorates of
Damascus and Damascus. And it adopted a researche
r on the
descriptive approach, and formed the research sample of (115)
guardian who have one child enrolled in a care for the disabled
institutes visually (public or private), and in one of the provinces
(Damascus or Damascus), and the researcher polled the views of
parents about The reality of the special services provided by
institutes of care for the visually impaired.
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