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Register a new userContribution in the serodiagnosis of visceral leishmaniasis in Syria by using the recombinant antigen (rK39)
إسهام في التشخيص المصلي لداء الليشمانية الحشوي (rK39) في سورية باستخدام المستضد المُخلّق
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سمر النحاس( باحث )
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نظراً للصعوبة التي يواجهها الأطباء في تشخيص داء الليشمانية الحشوي بسبب حداثة ظهوره في سورية، و تشابه أعراضه السريرية مع غيرها من الأمراض الأخرى (البر داء، التوكسوبلاسما، السل، البلهارسيا) حاولنا من خلال هذه الدراسة تأكيد على وجود إصابة بداء الليشمانية الحشوي عند بعض الأطفال من خلال الفحص المجهري للأطوار غير المسوّطة و ربط ذلك بتشخيص الإصابات باستخدام المستضد المخلّق (النوعي) لطفيلي الليشمانية الحشوي و ذلك أول مرة في سورية. أُخضع الأطفال المصابون بالليشمانية و قد سجلنا تراجعاً ملحوظاً الحشوية إلى المعالجة الدوائية بأملاح الأنتيموان (الغلوكانتيم في شدة الأعراض السريرية و انخفاضاً واضحاً في الكثافة الطفيلية المترافق مع انخفاض في نسبة الأضداد النوعية المتشكلة في مصول هؤلاء الأطفال المعالجين.
The diagnosis of visceral leishmaniasis (VL) remains problematic for physicians in Syria, because the appearance of this disease is new and its clinical criteria are similar to Malaria, Tuberculosis, and Toxoplasmosis symptoms. For this reason, we used the specific antigen (rK39) of Leishmania donovani complex, in order to certify the appearance of visceral leishmaniasis in some patients. Then we followed the infected cases with VL during treatment (15 days) and after treatment (10 days) with Glucantime®. We noted an improvement in clinical criteria and a significant decrease in the parasite numbers as well as in the specific L. donovani antibodies.
References used
Badaro R. Jones TC. & Lorenco R. A prospective study of visceral leishmaniasis in an endemic area of Brazil. J. Infect. Dis. (1986) 154: 639-49
World Health organization (WHO). Expert Committee on the control leishmaniasis. Control of the leishmaniasis. WHO Tech. Rep. Ser. (1990) 793: 139-58
Badaro R. Reed SG. & Carvalho EM. Immunofluorescent antibody test in American visceral leishmaniasis: Sensitivity and specificity of different morphological forms of two Leishmania species. Am. J. Trop. Med. Hyg. (1983) 32: 480-4
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In the present study, we tried to compare the sensitivity and the specificity
of the rK39 strips and DAT, to serodiagnose the visceral leishmaniasis disease
in some endemic villages in south of Syria, in order to apply the best and the
easy test i
n the epidemiological studies, for serodiagnosis this disease not only in
symptomatic patients but also in asymptomatic and suspected cases in order to
treat them early and rapidly.
Patients infected with different species of Leishmania in Syria develop
specific antibodies. For this reason, we have developed an immunodot assay for
the serodiagnosis of cutaneous and visceral leishmaniasis, which utilizes antihuman
IgG conjugat
ed with peroxidase (HRP) as the visualizing agent.
According to the high specificity and sensitivity of ELISA test, we tried in
this preliminary study, to certify the usefulness of ELISA test in the detection of
specific antibodies (IgG) to cutaneous leishmaniasis(CL) in SYRIA, in order to
certify
the diagnosis of this disease by using the classic methods (observation
microscopic – culture).
Visceral Leishmaniasis is still seen in developing country
To study the epidemiology of Visceral Leishmaniasis cases
hospitalized in Damascus University Children Hospital.
It was retro-spective study on (89) cases of Visceral
Leishmaniasis hospitalized between 1993 and 2005.
Green chemistry requires concepts and industrial ecology using natural systems to rid industrial water flow from heavy metals, which effects badly on health and environment.
For this purpose, the changes in coefficient of distribution of mercury has
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correlation of the following factors: Time of mixing phases, size of the developed steel, change the values of pH, concentration of elemental mercury, the concentration of calcium as a competitor to the occupation of sites available on the solid phase, the ratio V / M presents the water phase size on the mass of phase, which reflects the remaining time of water phase in the solid phase. The results show that pyrite can be proposed as safe environmental solution for purification of water phase of mercury, after controlling for some operation transactions.
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