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Register a new userSerodiagnosis of leishmaniasis «in Syria» using different immunological methods
التشخيص المناعي لليشمانية « في سورية » باستعمال تقانات مناعية مختلفة
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سمر النحاس( باحث )
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استعمل في هذه الدراسة عدد من التقانات المناعية المصلية للكشف عن الأضداد المتشكلة في مصول الأشخاص المصابين بداء الليشمانيات في سورية؛ فقد تم جمع المصول من أشخاص مصابين بهذا الداء، يعيشون في مناطق مختلفة من سورية، و من أشخاص أصحاء، يعيشون في مناطق موبوءة و غير موبوءة في سورية أيضًا؛ استعملت في هذه الدراسة المستضدات الطفيلية المنحلة لليشمانية، و كاشف ضد-الضد البشري IgG المعلم بالبيروكسيداز أو بالفوسفاتاز القلوية أو بالفلوريسئين (حسب الطريقة المطبقة).
Patients infected with different species of Leishmania in Syria develop specific antibodies. For this reason, we have developed an immunodot assay for the serodiagnosis of cutaneous and visceral leishmaniasis, which utilizes antihuman IgG conjugated with peroxidase (HRP) as the visualizing agent.
References used
Badaro R. Reed SG. Barral A. Orge G. Jones TC. Evaluation of the micro enzyme-linked immunosorbent assay (ELISA) for antibodies in American visceral leishmaniasis: antigen selection for detection of infectionspecific responses. Am. J. Trop. Med. Hyg1986
Dejeux Ph. & Landrivon G. Les leishmaniasis dans la Peninsule Arabique. Nouvelles Scientifique du France et du proche-Orient1997
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The diagnosis of visceral leishmaniasis (VL) remains problematic for
physicians in Syria, because the appearance of this disease is new and its
clinical criteria are similar to Malaria, Tuberculosis, and Toxoplasmosis
symptoms. For this reason, we
used the specific antigen (rK39) of
Leishmania donovani complex, in order to certify the appearance of
visceral leishmaniasis in some patients. Then we followed the infected
cases with VL during treatment (15 days) and after treatment (10 days)
with Glucantime®. We noted an improvement in clinical criteria and a
significant decrease in the parasite numbers as well as in the specific L.
donovani antibodies.
According to the high specificity and sensitivity of ELISA test, we tried in
this preliminary study, to certify the usefulness of ELISA test in the detection of
specific antibodies (IgG) to cutaneous leishmaniasis(CL) in SYRIA, in order to
certify
the diagnosis of this disease by using the classic methods (observation
microscopic – culture).
The genus Orchis L is one of the most important orchid tuberous species, distinguished by its versatility of food, medicinal and aromatic uses, in addition to its ornamental value. Due to the unjust collection processes that made many types of them rare or endangered, and the lack of information about their collected types and the specifications of their tubers.
قمنا من خلال هذا البحث بتغطية بعض الجوانب المناعية للطفيلي، حيث عزل طفيلي الليشمانية من
أشخاص مصابين بداء الليشمانية الجلدي، من منطقة الرحيبة (ريف دمشق)، ثم استنبت الطفيلي في
البداية على وسط صلب N.N.N بدرجة حرارة ٢٦ ° مئوية، و من ثم على وسط سائل ١٦
٤٠ RPMI- بغية
تعيين الشروط المثلى للاستنبات (تركيز الطفيلي الأولي، و حجم الوسط)، فحصلنا على منحنى نمو نموذجي
لدى استخدام التركيز ٥×٥^١٠ طفيلي/ميلي لترًا، حيث يمر الطفيلي بأطوار نمو واضحة و منفصلة. لاحظنا
أيضًا التبدلات الشكلية التي تطرأ على الطفيلي أمامي السوط promastigote في كل طور.
Malignancy is the second most common reason of death in most countries. Diagnosis of thyroid cancers is one of the most difficult medical problems. Therefore, it important to use diagnosing methods that bring about quick results, are less costly, and
prevent errors based on personal factors. An example of such new methods is the use of automated spectral imaging, which gives more information about the structure of tissue, through the formation of fingerprint histological diagnosis used in the automated machine.
This study included 25 cancer cases and 26 specimens of non-malignant thyroid or normal. The credibility of method is reflected in the high rates of correct diagnosis in cancer samples (TP) = 92% and the negative result in sections of non-malignant
(FN) = 96%. This method represents the use of automation technologies and artificial intelligence to facilitate and speed up the diagnosis of cancers and can be used to diagnose other organs and other tissues.
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