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Determination of the geometrical isomers of β- Carotene in palm oil using high performance liquid Chromatography (HPLC)

تحديد المماكبات الهندسية لمركب β-الكاروتين في زيت النخيل باستخدام تقانة الكروماتوغرافيا السائلة ذات الأداء العالي

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 Publication date 2004
  fields Chemistry
and research's language is العربية
 Created by Shamra Editor




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An HPLC separation of the geometric isomers of β-carotene was developed to evaluate the Provitamin A content of Palm oil . Different stationary phases were studied, the best separation of all-trans-, 9-cis- and 13-cis-β-carotene were obtained with calcium hydroxide columns using isocratic elution . Eluent was nhexane or isooctane or mixtures of them. These techniques were used to determine the provitamin A content of palm oil .

References used
Will, O.H. and Ruddat, U. LC Mag., 2 (1984) 610
Rodriguez-Amaya, D.B. Micronutr., J. 5 (1989) 191
Bauerfeind, J.C., Agric. J. Food Chem., 20 (1972) 456
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