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Register a new userDetermination of the geometrical isomers of β- Carotene in palm oil using high performance liquid Chromatography (HPLC)
تحديد المماكبات الهندسية لمركب β-الكاروتين في زيت النخيل باستخدام تقانة الكروماتوغرافيا السائلة ذات الأداء العالي
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Added by
Damascus University ورقة بحثية
Publication date
2004
fields
Chemistry
and research's language is
العربية
Created by
Shamra Editor
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يعد مركب β-الكاروتين أحد المشتقات السلفية precursors التي تعطي لدى أكسدتها في العـضوية الحية الفيتامين A . طورت طريقة جديدة لتعيين الكاروتينـات و مماكباتهـا الهندسـية باسـتخدام تقانـة الكروماتوغرافيا السائلة ذات الأداء العالي من أجل التعيين الأمثل لما يحتويه زيت النخيل من هذا المركب. درس في هذا البحث عدد من الأطوار الثابتة المنخفضة لفصل المماكبات الآتية: كاروتين – β – Cis -13 ، كاروتين – β – Cis -9 ، كاروتين- β -Trans و تم الحصول على أفضل النتائج باستعمال الأعمدة المحشوة بهيدروكسيد الكالسيوم باسـتخدام طـور متحرك من نظامي الهكسان أو ايزو الأوكتان أو مزيج منهما.
An HPLC separation of the geometric isomers of β-carotene was developed to evaluate the Provitamin A content of Palm oil . Different stationary phases were studied, the best separation of all-trans-, 9-cis- and 13-cis-β-carotene were obtained with calcium hydroxide columns using isocratic elution . Eluent was nhexane or isooctane or mixtures of them. These techniques were used to determine the provitamin A content of palm oil .
References used
Will, O.H. and Ruddat, U. LC Mag., 2 (1984) 610
Rodriguez-Amaya, D.B. Micronutr., J. 5 (1989) 191
Bauerfeind, J.C., Agric. J. Food Chem., 20 (1972) 456
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