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Register a new userThe effect of different concentrations of NaCl in seeds germination and early seedling growth of Lactuca plants cv. Kabous and Shinshar
تأثير مستويات مختلفة من كلور الصوديوم في إنبات البذور و مراحل النمو المبكرة لنبات الخس في صنفي كبوس و شنشار
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هدف البحث إلى دراسة تأثير مستويات مختلفة من الملوحة في معدل و نـسبة إنبـات بـذور الخـس .L sativa Lactuca صنفي شنشار و كبوس، و معرفة استجابة البادرات لهذه المستويات مـن الملوحـة (طول الجذور، طول البادرة، الوزن الطازج و الجاف للبـادرات). اسـتخدم كلوريـد الـصوديوم بتراكيـز (0 ،50 ،100 ،150 ،200 ميلي مول/ لتر)، وزرعت البذور في أطباق بتري، و سقيت بالتراكيز المختلفة من المحاليل الملحية. أدت الزيادة التدريجية في تراكيز الملوحة المدروسة إلى انخفاض تدريجي في جميع المؤشرات المدروسة في كلا الصنفين. كما أظهرت نتائج البحث أن الصنف كبوس أقل حساسية للملوحـة من الصنف شنشار، و تفوق أيضاً في المؤشرات المدروسة للبادرات (طول الجذور، طول السويقة الجنينية السفلى، الوزن الطازج و الجاف للبادرات) تحت ظروف الإجهاد الملحي.
This investigation was conducted at the laboratories of Horticulture Departments, Faculty of Agriculture to study the effect of different levels of salinity (NaCl) in the rate and the percentage of seed germination of lettuce, Lactuca sativa L. varieties: Shinshar and Kabous and to determine the seedling response to these levels of salinity with relative to root length, the length of the cold, fresh and dry weight of seedlings. NaCl was used at 0, 50, 100, 150 and 200 mM/l. Resulted showed that a gradual increase in salinity concentrations caused a gradual decline in all indicators studied in both cultivars. However, the plants of Kabous variety were less sensitive to the salinity and also higher than in the indicators studied for seedlings (rate of germination and percent, stems and roots length, fresh and dry weight of seedlings) under salt stress conditions.
Artificial intelligence review:
Research summary
هدفت هذه الدراسة إلى تقييم تأثير مستويات مختلفة من كلوريد الصوديوم (NaCl) على إنبات بذور ونمو بادرات صنفي الخس (Lactuca sativa L.) كبوس وشنشار. تم استخدام تراكيز مختلفة من NaCl (0، 50، 100، 150، 200 ميلي مول/لتر) وزرعت البذور في أطباق بتري وسقيت بالمحاليل الملحية المختلفة. أظهرت النتائج أن زيادة تركيز الملوحة تؤدي إلى انخفاض تدريجي في نسبة الإنبات، معدل الإنبات، طول الجذور، طول السويقة الجنينية السفلى، الوزن الطازج والجاف للبادرات في كلا الصنفين. ومع ذلك، تبين أن صنف كبوس أقل حساسية للملوحة مقارنة بصنف شنشار، حيث تفوق في جميع المؤشرات المدروسة تحت ظروف الإجهاد الملحي. تم تحليل البيانات باستخدام برنامج SPSS واستخدم اختبار دونكان للمقارنة بين المتوسطات.
Critical review
دراسة نقدية: الدراسة قدمت معلومات قيمة حول تأثير الملوحة على إنبات ونمو بادرات الخس، ولكن هناك بعض النقاط التي يمكن تحسينها. أولاً، كان من الممكن توسيع نطاق الدراسة ليشمل أصناف أخرى من الخس أو نباتات أخرى لتحسين الفهم العام لتأثير الملوحة. ثانياً، لم تتطرق الدراسة إلى الآليات الفسيولوجية والبيوكيميائية التي تفسر تفوق صنف كبوس على صنف شنشار تحت ظروف الملوحة، مما يترك فجوة في الفهم العميق للنتائج. أخيراً، كان من الممكن استخدام تقنيات حديثة مثل تحليل الجينات أو البروتينات لتقديم تفسير أكثر دقة لتأثير الملوحة على النباتات.
Questions related to the research
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ما هو الهدف الرئيسي من الدراسة؟
الهدف الرئيسي هو دراسة تأثير مستويات مختلفة من كلوريد الصوديوم على إنبات بذور ونمو بادرات صنفي الخس كبوس وشنشار.
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ما هي التراكيز المستخدمة من كلوريد الصوديوم في التجربة؟
التراكيز المستخدمة هي 0، 50، 100، 150، و200 ميلي مول/لتر.
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أي صنف من الخس كان أقل حساسية للملوحة؟
صنف كبوس كان أقل حساسية للملوحة مقارنة بصنف شنشار.
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ما هي المؤشرات التي تم قياسها في الدراسة؟
المؤشرات التي تم قياسها هي نسبة الإنبات، معدل الإنبات، طول الجذور، طول السويقة الجنينية السفلى، الوزن الطازج والجاف للبادرات.
References used
Ashraf, M. and A. Orooj. 2006. Salt stress effects on growth on accumulation and seed oil concentration in an arid zone traditional medicinal plant ajwain (Trachyspermum ammi L. Sprague). Journal of Arid Environments, 4: 209– 220
Bartha, C. 2012. Comparative study of physiological and molecular manifestations of salt stress tolerance in different intraspecific varieties of Lactuca sativa L. PhD Dissertation. Babes-Bolyai University (Faculty of Biology and Geology). Cluj-Napoc, Romania
Bartha, R. 1991. Microbial Ecology, In: Fundamental and Applications. Reading Addison Wesley
rate research
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The objective of this study is to compare the effect of five different Nacl
concentration (0,50,100,150,200 mM) on seeds germination and seedling growth of two
cultivars of soybean (sb-44,sb-172).
Seeds were germinated in petri dishes with sterile
filter paper moistened with 10 ml
of test solution at 25 c˚ in dark .
The obtained results showed that germination percentage , length of root and
hypocotyl were decreased with the increase of the Nacl concentration in the medium. The
best values was from cultivar sb-44 .
After seven days of incubation , significant differences were observed between
control and treated seeds .
Germinated seeds were subcultured on basal Murashige and Skoog medium solid at
the same Nacl concentration . The roots growth was mor affected by 200 mM Nacl
compared to seedling growth .
Plantlets were transfered into pots with nutrient soil , irrigated with saline water and
were adapted at laboratory . we obtained good plants grew to maturity in 10- 12 weeks .
The objective of this study is to compare the effect of five different Nacl concentration (0,50,100,150,200 mM) on seeds germination and seedling growth of two cultivars of soybean (sb-44,sb-172).
Seeds were germinated in petri dishes with sterile
filter paper moistened with 10 ml of test solution at 25 c˚ in dark .
The obtained results showed that germination percentage , length of root and hypocotyl were decreased with the increase of the Nacl concentration in the medium. The best values was from cultivar sb-44 .
After seven days of incubation , significant differences were observed between control and treated seeds .
Germinated seeds were subcultured on basal Murashige and Skoog medium solid at the same Nacl concentration . The roots growth was mor affected by 200 mM Nacl compared to seedling growth .
Plantlets were transfered into pots with nutrient soil , irrigated with saline water and were adapted at laboratory . we obtained good plants grew to maturity in 10- 12 weeks .
Different experiments were conducted under laboratory conditions, at the
Faculty of Agriculture – Damascus University, in order to develop a suitable
and efficient screening tool, which can be applied to assess the genetic
variability among 20 sor
ghum genotypes (Izraa series) for high salt stress
tolerance.
We were able to develop a screening technique involves pre- exposing
sorghum seedlings to a sub – lethal level of salinity stress (induction stress) (150
mM NaCl) for 16 hrs which can induce the genotypes to prepare defensive
means, which may differ according to the genetic potentiality of each genotype.
These protective responses may help the genotype to endure the lethal level of
salinity stress (400 mM NaCl) for 48 hrs. At a later stage, the seedlings were
transferred to distilled water for 72 hrs and the rate of recovery growth was
taken as a criterion to select the highly salt tolerant genotypes by using Zdistribution
analysis.
Results revealed that genotypes such as: Izraa 10, Izraa 15, and Izraa 18
could be classified as salt tolerant genotypes which showed higher recovery
growth and the least growth reduction over the control. Whereas a genotype
such as Izraa 22 was grouped as salt sensitive one where the percent growth
reduction over the control was greater, indicating that this genotype has low
recovery growth ability.
Our results suggest that the proposed screening technique is rapid, effective,
and match the natural screening.
A field experiment was carried out in Hout research station- Agriculture research center in
Swaida – General Commission For Scientific Research Agricultural during the growing
season of 2015-2016 to study the effect of different levels of deficit i
rrigation with
different levels of town refuse compost (TRC)on some morphological parameters and the
productivity of lettuce and compare it with the common mineral fertilizers.
The experiment was designed as a split block design with three levels of irrigation, each
level has four fertilizing treatments and control with three replicates.
The research has studied the plant height (H), the number of its leaves (N) ,the leaf area
(LAL), the plant's leaves area (LAP), the leaves area index (LAI), in addition to the lettuce
head's fresh weight and the total productivity of the planted area.
The irrigation and fertilizing interaction results indicate to the obvious positive effect of
the organic additions on the productivity parameters and the efficiency of deficit irrigation,
and the significant superiority of T11 treatment in all of the measured parameters in
compare with the other treatments with an increased final yield of %169 comparing with
T15 and % 428 comparing with T35.
The results also indicate to the possibility of saving %25 of the consumed amount of
irrigation water by using TRC instead of mineral fertilizer , and reveal that the organicmineral
combination achieved a better yield with percentages of ( 29- 44 – 53 )%
comparing with single mineral fertilizer in the three studied irrigation levels (T1-T2-T3)
respectively.
These results give the farmer a wide range of possibilities to choose what suits his
available productivity needs , and a clear idea about the expected yield according to the
chosen treatment.
This study aim at investigating the tolerance of two tomato rootstock (Sprit and ES-
30502) widely speared in Syria and used for crafting some tomato hybrids grown in green
houses for different salinity levels (0- 25- 50- 75- 100- 150 mM NaCl).
Th
e results show that the germination of rootstock Sprit seeds was not influenced by
high salinity 150 mM despite a delay of 1-2 days compared with the control. A reduction
of 15% was observed in the rootstock ES-30502 at 100 and 150 mM NaCl salinity levels,
in addition to slower percentage rate of germination. In contrast, the effect of salinity on
the development of the radical and rootlet was more pronounced in Sprit compared to ES-
30502. Salinity decreased the growth of both shoots and roots, and also decreased nutrient
acquisition in both rootstocks, but to a lesser degree in ES-30502. The uptake of K was not
greatly affected and concentrations in shoot remained normal. The concentrations of Ca
and Mg were also not affected by salinity. The most pronounced effect of salinity was on
nitrogen effect (NO3) which was reduced greatly in both rootstocks. This was accompanied
by accumulation of free NO3 ions in the shoot tissue (58 and 45 % of total N uptake at
salinity level of 150 mM). It is most likely that toxicity of Na and Cl ions, and consequent
osmotic changes in cell tissue led to slower nitrate reductase activity (NRA) and
accumulation of NO3.
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