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Register a new userEnhancement of in-vitro and in-vivo Human T Lymphocyte Activation via Cell Painting with Recombinant Chimeric Co-stimulatory B7-1:Fcγ1 Protein
تحسين تفعيل اللمفاويات الّتائية البشرية في الزجاج و في الحي بواسطة طلاء الخلايا بالبروتين الخيمري المأشوب B7-1:Fcγ1
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Damascus University ورقة بحثية
Publication date
2012
fields
Pharmacy
and research's language is
العربية
Authors
لمى يوسف( باحث )
Created by
Shamra Editor
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تعبر الخلايا اللمفاوية الّتائية عن جزيئات محرضة، مثل 1-B7 في بعض الحالات الفيزيزلوجية و المرضية، و لكن لم يتم حّتى تاريخه تقصي قدرة هذه الخلايا على تفعيل ذاتها. هدفت هذه الدراسة إلى اختبار إمكانية تعزيز تفعيل الخلايا الّتائية و تحسين تكاثرها في الزجاج و في الحي، و ذلك باعتماد تقنية لا جيّنية تعتمد الطلاء الخارجي لسطوح اللمفاويات التائية بالبروتين 1-B7 المأشوب بعد اقترانه بالبروتين A المنغرز في الغشاء الخلوي بذيلٍ من البالميتات/النخلات. أجريت هذه الدراسة في مخابر كلية الطب بجامعة بنسلفانيا في الولايات المتحدة الأمريكية و مخابر الهيئة العامة للتقانة الحيوية في دمشق في المدة الواقعة بين 2010-2012.
T cells express co-stimulatory molecules on their surfaces in both normal and pathologic settings; however, the possibility that expression of these co-stimulators may enable individual T cells to co-stimulate themselves has not been investigated. This study aims at assessing the possible potentiation of T cells activation and proliferation both in vitro and in vivo via enforced B7-1 expression on T lymphocytes using a non-genetic approach, i.e. cell painting, that comprises binding of recombinant B7-1 to plasma membrane-anchored palmitoylated protein A.
References used
Ledbetter JA, Imboden JB, Schieven GL, Grosmaire LS, Rabinovitch PS, Lindsten T, Thompson CB, June CH.CD28 ligation in T-cell activation: evidence for two signal transduction pathways.Blood. 1990;75(7):1531- 1539
Linsley PS, Brady W, Grosmaire L, Aruffo A, Damle NK, Ledbetter JA.Binding of the B cell activation antigen B7 to CD28 costimulates T cell proliferation and interleukin 2 mRNA accumulation.J Exp Med. 1991;173(3):721-30
Linsley PS, Ledbetter JA., The role of the CD28 receptor during T cell responses to antigen, Annu Rev Immunol 11 (1993) 191-212
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