In August 2015 the collected leaf of Arctostaphylos uva-ursi were
gathered. Then the volatile oil from fresh samples was extracted
with n-hexane and other samples with steam distillation.
In September 2014 the collected air-green samples of Fennel herb
were gathered. Then the volatile oil from fresh samples was
extracted with N-hexane and other samples with steam distilled
water.
In October 2014 the collected air-green samples of melissa
officinalis were gathered. Then the volatile oil from fresh samples
was extracted with N-hexane and other samples with steam
distillation.
Suitable conditions were chosen to separate the
components of the
volatile oil by GC-MAS and we noticed :
(25) compounds were found in the extracted volatile oil of the first
sample ( extraction by N-hexane ).
3 compounds of them are alcohol noncyclic MonoTerpine class and
they form 3,46% of the volatile oil weight, 1 alcohol cyclic
Monoterpine compound forms 1,83% of the volatile oil total
weight, 1 compound from cyclic DeTerpine class and it forms
2.31%, 2 compounds from cyclic MonoTerpine class and they form
3,14%, 2 compounds from Aldyhol group which form 52.03% from
the volatile oil's weight.
13 compounds from SesquieTerpine class with a 35,14% of the
total weight, 2 Ester compounds and they form 2,12%, and 1 acetic
compound which is Lenolenic acid with a 0,97% form the volatile
oil total weight.
This study aims to provide GC-FID and GC-MS analyses of the essential oil of dried aerial parts of Ecballium elaterium L. grown in Jordan and examining its cytotoxicity capacity. Methods: Essential oil was obtained by hydrodistillation using Clevenge
r apparatus. MTT assay method was used to investigate the plant's in vitro antiproliferative activity against MCF-7, Caco-2 and Panc-1 cancer cell lines in addition to normal fibroblast cells. Results: E. elaterium hydrodistilled oil yielded thirty one components, accounting for 76.3% of the total oil content. High contents of nonterpenoidal compounds, sesquiterpenes, and monoterpene characterized the volatile fractions with hinesol (17.2%), the principal compound, benzaldehyde (12.3%) and E-β-ionone (7.8%) as the major constituents. E. elaterium ethanolic extract showed good activity against MCF-7 and Caco-2 cells (IC50 values=29.67 μg/mL and 17.64 μg/mL, respectively). Moreover, all extracts were safe on normal human cells. In conclusion: Evaluation of E. elaterium volatile oil has been conducted for the first time in Jordan; also various extracts were tested for the first time against Panc-1 cells. Furthermore, based on the obtained results, ethanol extract of E. elaterium may be advocated as candidate for breast and colorectal cancers management.
