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Register a new userDetermination of Virulence Factors in Coagulase- Negative Staphylococci Isolated from Bovine mastitis
تحديد عوامل الضراوة عند العنقوديات سالبة المخثراز المعزولة من حالات التهاب ضرع عند الأبقار
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تضمنت هذه الدراسة عزل و تشخيص العنقوديات سالبة المخثراز من عينات الحليب التي جمعت من حالات التهاب ضرع سريري و تحت سريري عند الأبقار ( 180 بقرة سليمة ظاهريا و 130 بقرة مصابة بالتهاب الضرع ), و التي كشف عنها باستخدام اختبار كاليفورنيا ثم إجراء الزرع الجرثومي. تم عزل 368 عزولة عائدة للعنقوديات سالبة المخثراز بنسبة 45.77 % من مجمل حالات التهاب الضرع توزعت على الأنواع الثمانية التالية هي العنقودية الصباغية 80 (21.86%) و العنقودية كسيلوزس 86 (23.5) و العنقودية البشروية 65 (17.76%) و العنقودية المحللة للدم 64 (17.49%) و العنقودية سكيوري 32 (8.74%) و العنقودية الرمامة 19 (5.19%) و العنقودية البشرية 18 (4.91%) و العنقودية لينتوس 2 (0.55%).
This study include isolation and the identification of Coagulase – Negative Staphylococci from milk samples collected from clinical and sub-clinical bovine mastitis (180 apparently normal milk samples, and 130 bovine with clinical mastitis) , which is detected by CMT and bacteriological isolation 368(%45.77) isolates of CNS were obtained,which belonging to 8 species which were: S. chromogenes 80(21.86%) , S . xylosus 86 (23.5%), S. epidermidis 65 (17.76%),S.haemolyticus 64 (17.49%), S. sciuri 32 (8.74%), S. saprophyticus 19 (5.19%),S.hominis 18(4.91%), and S.lentus 2(0.55%).
References used
Akinjogunla, O. J.,Ajayi, A.O. and Ekeh, N.O.2014: Virulence factors and Antibiotic Resistant Staphylococcus spp from theAnterior Nares of Apparently Healthy Undergraduate Students in Uyo
Akinjogunla, O.J. and Enabulele, O. I. (2010). Virulence factors, plasmid profiling and curing analysis of multi-drug resistant Staphylococcus aureus and coagulasenegative Staphylococcus spp. isolated from patients with acute otitis media. J
Azih, A. and Enabulele, I. (2013). Species distribution and virulence factors of coagulase negative Staphylococci isolated from clinical samples from the University of Benin Teaching Hospital, Edo State, Nigeria. Jour Nat. l Sci Res,3(9): 38-43.Am. Sci., 6: 1022-1033
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This study was conducted to investigate of the subclinical mastitis
in dairy cows by using California Mastitis Test (CMT) and
microbial cultuer of positive California Mastitis Test milk sample .
880 quarter milk samples of 220 healthy cows were te
sted using ()
followed by bacteriological examination to identify the major
causative agents of intramammary infection.
Purpose: Lacunar infarcts is an important stroke subgroup with unique clinical and
pathologic features, but risk factors for lacunar infarcts have been rarely documented. To
address this matter, we studied 65 patients had lacunar infarction at Depa
rtment of
Neurology , Tishreen University Hospital , Lattakia , Syria , and 65 controls during the
period between May 2017 – May 2018 .
Methods: We obtained information concerning risk factor exposure status among the
patients by a structured questionnaire , we recorded age , sex , blood pressure , glucose ,
heart diseases , cigarette smoking , alcohol drinking , physical exercise for patiens and
controls .We do laboratory tests and ECG and echocardiography and CT brain or MRI .
Results: Significantly increasing the risk of lacunar stroke were hypertension
(P-value=0.0001) and (OR=9.9), Current smokers (P-value=0.002) and (OR=5.2), diabetes
(P-value=0.001) and (OR=5.3) , whereas frequent physical exercise was associated with a
significantly decreased risk) P-value=00001( and (OR=2.6). There was no risk of
lacunar stroke associated with heart disease (P-value=0.6) (OR=0.8) , high cholesterol (Pvalue=
1) and (OR=1) , alcohol drinking (P-value=0.7) and (OR=0.8).
Conclusions: Patients with hypertension or diabetes, current smokers, those who
have not heart disease, are at a higher risk of lacunar stroke, whereas those who undertake
regular physical exercise may be at lower risk.
Fifty-one female and thirty-three male patients who suffered from UTI and
attended Al-Thawra General Hospital at Ibb City in Yemen, were examined
for the effects of different antibiotics on E. coli isolated from the mid-stream
urine samples.
The
results showed a variable degree of resistance revealed by E. coli
isolates. It ranged between ١٠٠٪ ((against Penicillin G and Ampicillin) and zero
resistance ((against Kanamycin, Ciprofloxacin, and Gentamicin). Other tested
antibiotics showed resistance ranging between ٤٠ and ٣,٣٤٪.
In the framework of this research, bacteria belonging to the genus Bacillus were isolated from some Syrian soils in the governorate of Damascus and its countryside, and the governorates of Daraa and Quneitra. Some morphological, agricultural and bioc
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were collected during the years 2012-2013 where 13 were isolated from soil, 23
from tree leaves and 14 from food. The isolates AUX system. Were classified
into three Rhodoto
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76%, 20% and 4% respectively using API 20c. Six methods were applied in
breaking down the yeast cells and extracting the carotenoids. It was shown the
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to the yeast biomass and incubating for 24 hrs at 4˚C yielded in higher
quantities when compared with the other five methods. All isolates were able to
produce carotenoides but they varied in their efficiency where the isolate A24
(R.mucilaginosa) isolated from food was distinguished by its high level of
production which reached 658.23 μg/g dry weight compared with the others.
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