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Register a new userHistopathological study for diagnosis of Cryptosporidiosis in broiler breeder flocks in Syria
دراسة تشريحية و نسيجية لتشخيص داء البوغيات الخفية في قطعان أمهات اللحم في سوريا
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أنجزت هذه الدراسة على قطعان أمهات اللحم بعمر 1-4 أشهر, و بحسب تاريخ الحالات المرضية كانت الطيور تعاني من أعراض تنفسية مزمنة و عدم استجابة للمعالجات الدوائية المختلفة وضعت في التحويل الغذائي و فقدان الوزن و زيادة نسبة النفوق.
This study was made on broiler breeder flocks 1-4 months of age. According to flock’s data, birds suffered from chronic respiratory disease, treatment failure, loss of weight and increasing mortality.
References used
Al-Kennany E R , Rahawi A M and Al-Jabori S A. Histopathological and Histochemical Study of Intestinal Cryptosporidiosis in Mice. Kufa Journal for veterinary medical science vol No.2(2012) 36-44
Casanova S, Verdes JM, Okada K.Cryptosporidium spp. in bursa of Fabricius of broiler chickens from Uruguay. Ciência Rural, Santa Maria, v.45, n.1, p.64-67, jan, 2015
Chvala S, Fragner K, Hackl R, Hess M, Weissenböck H. Cryptosporidium infection in domestic geese (Anser anser f. domestica) detected by in-situ hybridization. J Comp Pathol. 2006 Feb-Apr;134(2-3):211-8
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Two hundred fecal samples were randomly collected from various
aged broiler flocks farms, which suffered from diarrhea or
respiratory problem or both in Tartous and Latakia Provinces of
Syria.
This study demonstrated the infection of the broiler
flocks with
Cryptosporidium in Tartous Province of Syria with a rate of 5.6%
only.
The results of tests based on morphology and size of
Cryptosporidium oocysts showed that the parasite is probably
C. baileyi which ranged in size about (6 μm X 4.5 μm).
It was collected 2990 blood samples from various ages of chicken of
broiler, layer and breeder flocks distributed from different regions of
SAR, in order to detect Mycoplasma Galisepticum that used in Rapid
sero- agglutination test and indirect EL
ISA test. The study reported seroprevalence
of positive cases using rapid sero- agglutination test 33% in
broiler breeder, 1% in broiler chicken and 43% in layer chicken and
overall prevalence 24%. And by using ELISA test, the reported seroprevalence
values 27% in breeder, 8% in broiler and 33% in layer
chicken and overall-prevalence 19%.
This study was carried out on 90 broiler chicks which divided into three groups. The first group has been given 0.75 g/L potassium nitrate and the second group was given 1 g/L potassium nitrate with drinking water for 30 days, While the third group w
as regarded as a control.
The birds were reared in same keeping house and feeded with the same ration without any additives and no vaccinations were applied.
All birds were sacrificed and examined by naked eye. Then the thymus gland was fixed by formalin and stained microscopic sections were examined by microscope.
The histological changes in the birds which dosed by 1 g/L and 0.75 g/L of nitrate were similar and the observed changes were the small size of cortex in some lobules, and the cortex were separated from bulb with increase the number of Hassall bodies in the bulb region, also there were vacuoles and reticular cells in different stages of degeneration and edema in the connective tissues in comparison with control group.
These results confirmed that nitrate had toxic effects on the tissues of thymus, and as a sequence reduced the number of T cells which lead to decrease the resistance and Immune response of the birds and retarded the growth rate.
A reverse transcriptase-polymerase chain reaction (RT-PCR) technique was used
to detect infectious bronchitis virus (IBV) in the commercial broiler flocks in
Syria. 50 tissue samples were taken from tracheal tissue, trachea and kidney of
the broil
ers suspected of infectious bronchitis (IB) from different governorates
of Syria i.e. Latakia, Tartous, Hama and Damascus countryside. RNA was
extracted directly from the tissue samples and then RNA was converted to cDNA
by RT-PCR technology; PCR reaction and Nested PCR interaction were carried
out sequentially. The primers used in the RT-PCR reaction were selected from
the S1 gene (spike), where mutations of the virus genome were concentrated in
this region (the hypervariable region). Some positive samples (10) were injected
at an age of 9-11 days old SPFEE-specific pathogen free embryonated eggs
according to the methods adopted in virology. This research was carried out at
the laboratories of Latakia Research Center, General Commission for Scientific
Agricultural Research GCSAR, in cooperation with the PCR laboratory at the
Faculty of Veterinary Medicine in Hama. The results showed the existence of 37
positive case for RT-PCR (74%), and the infectious embryos showed clear and
characteristic anatomical lesions of the infectious bronchitis virus after 5-6 days
post injection, delayed and undeveloped fetal (dwarfism), fingertip entanglement
and hemorrhage compared with the negative control. The results also showed the
sensitivity and speed of the RT-PCR test in the detection of the IBV virus.
يقوم هذا البحث باجراء دراسة وبائية للأنماطو تحت الأنماط النسيجية للمفومالاهودجكن المشاهدة في مشفى مركزي تعليمي هو مشفى المواساة و ذلك في العقد اللمفاوية و في الأعضاء خارج العقد اللمفاوية بشكل بدئي أو توضعات ثانوية و معرفة نسبها و توزعها في كل الأعمار
و القاء الضوء على أهمية و دور التلوينات المناعية النسيجية الكيميائية في وضع تشخيص نهائي و ذلك وفقا لتصنيف عام 2008.
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