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Register a new userStudy of 15 System of Short Tendem Repeats Loci (STRs) In Sample of Syrian Population
دراسة 15 نظاماً من أنظمة التكرارات الترادفية القصيرة STRs لدى عينة من الشعب السوري
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Damascus University ورقة بحثية
Publication date
2015
fields
Medicine
and research's language is
العربية
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Shamra Editor
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خلفية البحث و هدفه: درس 15 موضعاً مورثياً (نظاماً) من الموضوعات المورثية للتكرارات الترادفية القصيرة (STRs) على عينة من الشعب العربي السوري القاطنين في مدينة حلب. مواد البحث وطرائقه: درس 15 موضعاً مورثياً (نظاماً) من أنظمة التكرارات الترادفية القصيرة (STRs) على عينة من مجمل أطياف الشعب العربي السوري القاطنين في حلب، والمكونة من 127 شخصاً سليماً باستخدام كيتات من شركة Promega المكونة من مجموعة الأنظمة (D3S1358, TH01, D21S11, D18S51, PentaE, D5S818, D13S , 31.D7S820, D16S539, CSF1PO, PenyaD, vWA, D8S1179, T, POX , FGA). و استخرج ال DNA من العينات باستخدام كيت كياجين DNA Mini Kit بحسب التعليمات المرافقة للكيت، أجري التضخيم بتقانة ال PCR بجهاز الحرارة الدورية Thermocycler لشركة Applied Biosystems و الترحيل بجهاز الشركة ذاتها ABI PRISM 310 Genetic Analyzer ثم قرئت النتائج ببرنامج GeneMapper ID v3.0 software . أجري على العينات دراسة لمجموعة من المعايير الإحصائية الأساسية للأنظمة الخمسة عشر مثل حساب تخالفية اللواقح , و التعددية الشكلية PIC , و قوة التفريق PD وقوة الإقصاء PE ،و مؤشر الأبوة باستخدام برنامج إكسل –ويندو 2007 لشركة سوفت وير. وحسبت قيم الانحراف المعياري p-value للتأكد من خضوع التوزع المورثي للأنظمة المدروسة على العينة المنتقاة لتوازن هاردي وواينبرغ. النتائج: أظهرت دراستنا المقارنة بدراسات مشابهة لدى الشعب المغربي و التركي و العراقي فائدة الأنظمة المدروسة في التطبيقات الطبية الشرعية، و في تحديد هوية الأشخاص في سورية. الاستنتاج: فائدة الأنظمة المدروسة في التطبيقات الطبية الشرعية، و في تحديد هوية الأشخاص في سورية.
Background& Objective: The aim of this study was to know the Allele frequencies for the 15 STR loci in the sample in Aleppo in Syria. Materials & Methods: Allele frequencies for the 15 STR loci included in the PowerPlex16 PCR Amplification Kit panel from (Promega, Madison, USA) according to manufacturer’s instructions (D3S1358, TH01, D21S11, D18S51, PentaE, D5S818, D13S317, D7S820, D16S539, CSF1PO, PenyaD, vWA, D8S1179, T, POX , FGA) and several statistical parameters were estimated from a sample of 127 unrelated individuals representing all ethnic groups in Aleppo in Syria . DNA was extracted from blood samples using QIAmp1 DNA Mini Kit (QIAGEN, Hagen, Germany) according to manufacturer’s instructions and the PCR products were typed using an ABI PRISM 310 Genetic Analyzer (Applied Biosystems, Foster City, CA, USA)). The results were analyzed using GeneMapper ID v3.0 software (Applied Biosystems). Several forensic and population parameters such as heterozygosity, polymorphic information content (PIC), power of discrimination (PD), power of exclusion (PE) and the paternity index (PI)) were calculated using Microsoft Excel-2007. A p-value of the 15 loci were estimated by using the statistical Microsoft Windows 2007 to verify whether the genotype distribution conformed to Hardy- Weinberg equilibrium predictions. Results : We compared our data with other data sets from published studies of individuals from Turkey, Morocco and Iraq, our data indicate that standardized multilocus STR panels may be a useful forensic tool which can be applied for identification purposes also in the Syria population. Conclusion: Our Data may be a useful forensic tool which can be applied for identification purposes also in the Syria population.
References used
Ellegren, H Microsatellites: simple sequences with complex evolution. Nat. Rev. Genet. 2004; 5:435–445
Chakraborty R, Jin L. Heterozygote deficiency, population substructure and their implications in DNA fingerprinting. Hum Genet 1992;88: 267-272
Evett, I. W and Gill, P. Population geneticsof short tandem repeat (STR) loci. Genetica 1995; 96:69-87
Edwards, M.C. and Gibbs, R.A. Multiplex PCR: advantages, evelopment, and applications. [Review]. PCR Methods &.Applications1994; 3: S65-S75
Butler JM Forensic DNA Typing Biology& Technology behind STR Markers ; Academic Press,USA. ; 2001
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As well as to detect whether there are differences in the
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Materials and Methods: The orthodontic records of 623 patients ag
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prevalence of patients with one or more permanent missing teeth.
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anterior teeth missing than posterior teeth (P=0.009).
Conclusions: The prevalence of hypodontia showed in this study was within the
range published in the literature. The patterns are similar to most reported patterns in the
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