A field survey for chickpea wilt disease Fusarium
oxysporum f.sp ciceris was conducted in different provinces
of north Syria during 2011 cropping season with the
objectives to determine chickpea wilt distribution and factors
affecting its develop
ment. Two hundred and fifty samples
were collected from Alhasakah, Aleppo and Idleb fields. The
results showed varying levels of incidence between and
within provinces (2-90%), and the highest incidence was
recorded in Alfouaa- Idleb (90%). The incidence and
distribution of this disease seemed to be affected by many
factors especially date of sown and soil kind. The
pathogenicity test of 60 isolates related to Fusarium
oxysporum on susceptible cultivars Ghab1 was shown that 54
isolates related to Fusarium oxysporum f. sp. ciceris; 32
isolates of them were caused yellowing symptoms and 22
were caused wilting symptoms.
This study aimed to investigate the possibility of rapid identification of
isolated bacteria Leuconostoc from Syrian white fresh cheese made of cow and
ewe milk using Fourier transform infrared spectroscopy (FTIR). 20 isolates (14
from ewe milk ch
eese and 6 from cow milk cheese) were isolated. These isolates
were pretasted with PCR technique using specific primers. Results indicated
that these isolates are belonged to the genus Leuconostoc and 12 of them are
belonged to Ln. mesenteroides species. The results of FTIR technique showed in
comparison to device library that all isolates are belonged to genus
Leuconostoc, and 4 of these isolates are belonged to Ln. citreum species which
could not be identified using PCR method (with the used primers), and helped
to identify the following subspecies: Ln.mesenteroides subsp mesenteroides and
Ln. mesenteroides subsp dextranicum while PCR technique described them
belonging only to a Ln. mesenteroides species. Moreover, FTIR technique was
able to give the results within 25 hours, thus it can be used in identification of
food bacteria more quickly.
