هدفت الدراسة إلى بحث إمكانية التحديد السريع لبكتيريـا Leuconostoc المعزولـة مـن الأجبـان
البيضاء الطازجة السورية المصنعة من حليب البقر (6 عينات) و حليب الغنم (20 عينة) باستخدام تقنيـة
تحويل فوريير للأشعة تحت الحمراء (FTIR) . و بينت تقنيـة تفاعـل التسلـسل البـوليمرازي (PCR)
باستخدام مرئسات خاصة، أنها تتبع للجنس Leuconostoc . و تبين أن 12 عزلة منهـا تابعـة للنـوع
mesenteroides.Ln . ثم اختبرت هذه العزلات باستخدام جهاز IR-FT ، فتبين بالمقارنـة مـع مكتبـة
الجهاز، أن العزلات جميعها تتبع للجنس Leuconostoc . و أن 4 عـزلات منهـا تابعـة للنـوع .Ln
citreum في حين لم يمكن و اعتمادا لى تقنية (PCR) و وفقاً للمرئسات المستخدمة تحديدها، كما ساعدت
Ln.mesenteroides و Ln.mesenteroides subsp mesenteroides الأنـواع تحـت تحديـد في
dextranicum subsp ، التي اكتفت تقنية PCR بالإشارة إلى أنها تتبع للنوع mesenteroides.Ln.
و مكنًت تقنية IR-FT من تحديد البكتيريا خلال فترة لا تتعدى 25 ساعة. و من الممكن استخدامها للكشف
عن البكتيريا في الأغذية.
This study aimed to investigate the possibility of rapid identification of
isolated bacteria Leuconostoc from Syrian white fresh cheese made of cow and
ewe milk using Fourier transform infrared spectroscopy (FTIR). 20 isolates (14
from ewe milk cheese and 6 from cow milk cheese) were isolated. These isolates
were pretasted with PCR technique using specific primers. Results indicated
that these isolates are belonged to the genus Leuconostoc and 12 of them are
belonged to Ln. mesenteroides species. The results of FTIR technique showed in
comparison to device library that all isolates are belonged to genus
Leuconostoc, and 4 of these isolates are belonged to Ln. citreum species which
could not be identified using PCR method (with the used primers), and helped
to identify the following subspecies: Ln.mesenteroides subsp mesenteroides and
Ln. mesenteroides subsp dextranicum while PCR technique described them
belonging only to a Ln. mesenteroides species. Moreover, FTIR technique was
able to give the results within 25 hours, thus it can be used in identification of
food bacteria more quickly.
References used
Amiel C., L. Mariey, M. C. Curk-Daubie and J. Travert, 2000. Potentiality of Fourier Transform Infrared Spectroscopy (FTIR) for discrimination and identification of dairy Lactic acid bacteria. Le Lait, 80: 445-459
Barreau C. and G. Wagener. 1990. Characterisation of Leuconostoclactis strains from human sources. Journal of Clinical Microbiology. 28:1728-1733
Bulut C., 2003. Isolation and molecular characterization of lactic acid bacteria from cheese. Biotechnology and Bioengineering, İzmir Institute of Technology, Master of Science. Turkey
This research aimed to identify the bacteriocin produced from a strain of
Strep. Thermophiles which were isolated from native Syrian white cheese and
to identify its characteristics.
The study showed that the produced bacteriocin is able to inhibi
The objective of this investigation was the Identification of Salmonella spp.
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80 samples of white cheese (traditional processed
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