The antibacterial activity of Golden henbane (Hyoscyamus aureus) on
several species of bacteria: Pseudomonas aeruginosa, Staphylococcus
aureus and Escherichia coli was determined. Samples of H. aureus
plants were collected from six locations in southern Syria. The extract
of roots and leaves of H. aureus were prepared at concentrations.
This research aimed to study the effect of various growth conditions
(temperature, culture age and culture medium) on the fatty acids composition
of cytoplasm membrane of 37 isolates of Staphylococcus aureus using Gas
Chromatography-Mass Spectrome
try (GC-MS). Results revealed that the total
number of fatty acids of Staph. aureus was 33. Eight of them were principle and
included: anti-isotetradecanoic acid (aC15:0), octadecanoic acid (C18:0),
eicosanoic acid (C20:0), isotetradecanoic acid (Ci14:0), isotridecanoic acid
(Ci15:0), hexadecanoic acid (C16:0), antisohexa- decanoic acid (Ca17:0) and
octadecenoic acid (C18:1) with a percentage of 33.96, 18.37, 14.94, 7.32, 6.23,
4.14, 3.03 and 2.89%, respectively. The percentage of each of the other fatty
acids was less than 1%. Increasing the incubation temperature was associated
with reducing the percentages of Ca15:0, Ci15:0, C16:0 and C18:1; and
increasing Ca17:0, Ci14:0, C18:0 and C20:0. The increase in incubation period
caused a decrease in C16:0, C18:0, C18:1 and C20:0; and an increase in Ca15:0
and Ca17:0. Moreover, the variation in culture media caused differences in the
percent of Ca15:0, C18:0 and C20:0; while the other fatty acids showed no
changes.
This study was conducted in the years 2008-2009-2010, it has analyzed for
Staph. aureus and isolated from 100 samples of traditional ice cream sold to
customers it has had collected from different regions parts of Damascus and his
countryside. The
study has been found that there was no violation of the Syrian
standard for Staph. aureus. And the results were also diagnosed using the
techniques of API Staph and PCR equal.
54 isolates were isolated of the bacteria associated with ear infections which
have antimicrobial resistance from the patients in the National Hospital in
Qamishly City during the period from 01/08/2008 to 31/10/ 2009. The number
of Pseudomonas ae
ruginosa was high, followed by Staphylococcus aureus. It has
been found that the rate of ear infections was lower in male (44.4%) than
female (55.5%), and was (48.10%) in the first age category (1- 15 year) more
than the second age category (15- 30 year) (31.37%), while the rate of ear
infections was (23.5%) in the third age category(30- 60 year ).
We found that all the strains of isolated bacteria showed high susceptibility
to Imipenem (100%), and all bacteria of Pseudomonas, klebseilla, Proteus,
Enterobacter, showed high sensitivity to ciprofloxacin, levofloxacin (85%), but
only staphylococcus (58%).
In addition, most isolated bacteria showed intermediate sensitivity to
tobramicine, amikacine, gentamycine, and cephalosporinses like ceftazidime,
cefaclor and cefotaxime.
At the same time, most isolated bacteria showed resistance to Amoxicilline,
Ampicillin, Penicillin, Oxacylline, Sulphamethoxasole, erythromycin,
vancomycine and tetracyclines.
