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Antibacterial Activity of the Leaves and Root Extracts of Hyoscyamus aureus

الفعالية المضادة للبكتريا لأوراق و جذور نبات البنج الذهبي Hyoscyamus aureus

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 Publication date 2016
and research's language is العربية
 Created by Shamra Editor




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The antibacterial activity of Golden henbane (Hyoscyamus aureus) on several species of bacteria: Pseudomonas aeruginosa, Staphylococcus aureus and Escherichia coli was determined. Samples of H. aureus plants were collected from six locations in southern Syria. The extract of roots and leaves of H. aureus were prepared at concentrations.


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Research summary
تستعرض هذه الدراسة الفعالية المضادة للبكتيريا لمستخلصات أوراق وجذور نبات البنج الذهبي (Hyoscyamus aureus) ضد ثلاثة أنواع من البكتيريا: Pseudomonas aeruginosa و Staphylococcus aureus و Escherichia coli. تم جمع العينات النباتية من ست مناطق في جنوب سوريا، وتم تحضير المستخلصات النباتية بتراكيز مختلفة (100، 150، 200، 250، 300، 350 ميكروغرام/مل). أظهرت النتائج أن مستخلص الجذور كان أكثر فعالية من مستخلص الأوراق في تثبيط وقتل البكتيريا المدروسة. تراوحت قيم التركيز الأدنى المثبط للنمو (MIC) والتركيز الأدنى القاتل (MBC) للبكتيريا P. aeruginosa بين 100-200 و100-250 ميكروغرام/مل على التوالي، بينما تراوحت قيم MIC وMBC للبكتيريا S. aureus بين 100-300 و150-350 ميكروغرام/مل، وللبكتيريا E. coli بين 150-300 و200-350 ميكروغرام/مل. كما أظهرت الدراسة أن الفعالية المضادة للبكتيريا تتأثر بالمنطقة الجغرافية التي جُمع منها النبات.
Critical review
تقدم هذه الدراسة مساهمة قيمة في فهم الفعالية المضادة للبكتيريا لنبات البنج الذهبي، إلا أن هناك بعض النقاط التي يمكن تحسينها. أولاً، لم يتم توضيح الآليات البيوكيميائية التي تجعل مستخلص الجذور أكثر فعالية من مستخلص الأوراق، مما يترك مجالاً لمزيد من البحث في هذا الجانب. ثانياً، الدراسة اقتصرت على ثلاثة أنواع من البكتيريا، ويمكن توسيعها لتشمل أنواع أخرى لتقديم صورة أكثر شمولية. ثالثاً، لم يتم مناقشة تأثير العوامل البيئية الأخرى مثل التربة والمناخ على الفعالية المضادة للبكتيريا، وهو ما يمكن أن يكون له تأثير كبير. وأخيراً، يمكن تحسين منهجية التحليل الإحصائي لضمان دقة النتائج.
Questions related to the research
  1. ما هي الأنواع البكتيرية التي تم اختبارها في الدراسة؟

    تم اختبار ثلاثة أنواع من البكتيريا: Pseudomonas aeruginosa و Staphylococcus aureus و Escherichia coli.

  2. ما هو التركيز الأدنى المثبط للنمو (MIC) للبكتيريا Pseudomonas aeruginosa؟

    تراوح التركيز الأدنى المثبط للنمو (MIC) للبكتيريا Pseudomonas aeruginosa بين 100-200 ميكروغرام/مل.

  3. ما هو الفرق بين فعالية مستخلص الجذور ومستخلص الأوراق؟

    أظهرت الدراسة أن مستخلص الجذور كان أكثر فعالية من مستخلص الأوراق في تثبيط وقتل البكتيريا المدروسة.

  4. كيف تؤثر المنطقة الجغرافية على الفعالية المضادة للبكتيريا لنبات البنج الذهبي؟

    أظهرت الدراسة أن الفعالية المضادة للبكتيريا تتأثر بالمنطقة الجغرافية التي جُمع منها النبات، حيث لوحظت فروق في قيم MIC وMBC حسب المنطقة.


References used
ALVAREZ, M. A. and MARCONI, P. L., 2011- Genetic transformation for metabolic engineering of tropane alkaloids, Intech, 291-304
BAHMANZADEGAN, A.; SEFIDKN, F. and SONBOLI, A., 2009- Determination of hyoscyamine and scopolamine in four Hyocyamus species from Iran, Iranian Journal of Pharmaceutical Research, vol. 8. 65-70
BESHER, S.; Al-AMMOURI, Y. and LAWAND, S., 2012- Determination of tropane alkaloids in golden henbane (Hyoscyamus aureus) in vitro, Віснᴎk ykpAїHcbkoгo товаpᴎctBA генетиків і селекöіонерів, vol. 10(2). 231-336
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This research was conducted to detect the morphological and molecular differences between golden henbane (Hyoscyamus aureus L) collected from south Syria. Seeds were collected from six locations, then sterilized and grown In Vitro. Grown plants we re described for some morphological characters. The molecular characterization was carried out using the method of Inter Simple Sequence Repeats (ISSR) to detect genetic variations. The plants showed significant (p<0.01) morphological differences among studied locations, Plant height ranged from 14.97 to 18.97 cm and stem color ranged from red to green. Trichome density also ranged between medium, high and very high. Furthermore, significant differences in leaves dimensions were observed according to the plant geographical location. The main root length ranged from 6.09 to 8.37 cm. Molecular analysis by ISSR produced a total of 56 bands, 49 of which were polymorphic (87.5%), resulted from using 11 non-specific primers. A genetic distance dendrogram was drawn. based on the results of genetic characterization of plant from different locations. The plants collected from Salkhad separated with a genetic distance of 0.312 while the plants collected from other locations were grouped together, at the same time, they were divided into two sub groups, the first group comprised plants collected from Qalamoun and Dimas regions with a genetic distance of 0.032, whereas sub group II content Bosra plants which separated clearly from those of Dara' dam and Wadi Al-Zaidi by a genetic distance of 0.017, These results may indicate a relationship between genetic characters of this plant species with geographic distribution.
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