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Register a new userAntibacterial Activity of the Leaves and Root Extracts of Hyoscyamus aureus
الفعالية المضادة للبكتريا لأوراق و جذور نبات البنج الذهبي Hyoscyamus aureus
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تم تنفيذ هذا البحث لاختبار فعالية نبات البنج الذهبي المضادة للميكروبات على عدة أنواع بكتيرية: Staphyllococcus aureus ,Pseudomonas aeruginosa, Escherichia coli. تم جمع عينات نبات البنج من ست مناطؽ في جنوب سورية, وحُضِرت المستخلصات النباتية من جذور و أوراق هذه النباتات بعدة تراكيز.
The antibacterial activity of Golden henbane (Hyoscyamus aureus) on several species of bacteria: Pseudomonas aeruginosa, Staphylococcus aureus and Escherichia coli was determined. Samples of H. aureus plants were collected from six locations in southern Syria. The extract of roots and leaves of H. aureus were prepared at concentrations.
Artificial intelligence review:
Research summary
تستعرض هذه الدراسة الفعالية المضادة للبكتيريا لمستخلصات أوراق وجذور نبات البنج الذهبي (Hyoscyamus aureus) ضد ثلاثة أنواع من البكتيريا: Pseudomonas aeruginosa و Staphylococcus aureus و Escherichia coli. تم جمع العينات النباتية من ست مناطق في جنوب سوريا، وتم تحضير المستخلصات النباتية بتراكيز مختلفة (100، 150، 200، 250، 300، 350 ميكروغرام/مل). أظهرت النتائج أن مستخلص الجذور كان أكثر فعالية من مستخلص الأوراق في تثبيط وقتل البكتيريا المدروسة. تراوحت قيم التركيز الأدنى المثبط للنمو (MIC) والتركيز الأدنى القاتل (MBC) للبكتيريا P. aeruginosa بين 100-200 و100-250 ميكروغرام/مل على التوالي، بينما تراوحت قيم MIC وMBC للبكتيريا S. aureus بين 100-300 و150-350 ميكروغرام/مل، وللبكتيريا E. coli بين 150-300 و200-350 ميكروغرام/مل. كما أظهرت الدراسة أن الفعالية المضادة للبكتيريا تتأثر بالمنطقة الجغرافية التي جُمع منها النبات.
Critical review
تقدم هذه الدراسة مساهمة قيمة في فهم الفعالية المضادة للبكتيريا لنبات البنج الذهبي، إلا أن هناك بعض النقاط التي يمكن تحسينها. أولاً، لم يتم توضيح الآليات البيوكيميائية التي تجعل مستخلص الجذور أكثر فعالية من مستخلص الأوراق، مما يترك مجالاً لمزيد من البحث في هذا الجانب. ثانياً، الدراسة اقتصرت على ثلاثة أنواع من البكتيريا، ويمكن توسيعها لتشمل أنواع أخرى لتقديم صورة أكثر شمولية. ثالثاً، لم يتم مناقشة تأثير العوامل البيئية الأخرى مثل التربة والمناخ على الفعالية المضادة للبكتيريا، وهو ما يمكن أن يكون له تأثير كبير. وأخيراً، يمكن تحسين منهجية التحليل الإحصائي لضمان دقة النتائج.
Questions related to the research
-
ما هي الأنواع البكتيرية التي تم اختبارها في الدراسة؟
تم اختبار ثلاثة أنواع من البكتيريا: Pseudomonas aeruginosa و Staphylococcus aureus و Escherichia coli.
-
ما هو التركيز الأدنى المثبط للنمو (MIC) للبكتيريا Pseudomonas aeruginosa؟
تراوح التركيز الأدنى المثبط للنمو (MIC) للبكتيريا Pseudomonas aeruginosa بين 100-200 ميكروغرام/مل.
-
ما هو الفرق بين فعالية مستخلص الجذور ومستخلص الأوراق؟
أظهرت الدراسة أن مستخلص الجذور كان أكثر فعالية من مستخلص الأوراق في تثبيط وقتل البكتيريا المدروسة.
-
كيف تؤثر المنطقة الجغرافية على الفعالية المضادة للبكتيريا لنبات البنج الذهبي؟
أظهرت الدراسة أن الفعالية المضادة للبكتيريا تتأثر بالمنطقة الجغرافية التي جُمع منها النبات، حيث لوحظت فروق في قيم MIC وMBC حسب المنطقة.
References used
ALVAREZ, M. A. and MARCONI, P. L., 2011- Genetic transformation for metabolic engineering of tropane alkaloids, Intech, 291-304
BAHMANZADEGAN, A.; SEFIDKN, F. and SONBOLI, A., 2009- Determination of hyoscyamine and scopolamine in four Hyocyamus species from Iran, Iranian Journal of Pharmaceutical Research, vol. 8. 65-70
BESHER, S.; Al-AMMOURI, Y. and LAWAND, S., 2012- Determination of tropane alkaloids in golden henbane (Hyoscyamus aureus) in vitro, Віснᴎk ykpAїHcbkoгo товаpᴎctBA генетиків і селекöіонерів, vol. 10(2). 231-336
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This research was conducted to detect the morphological and molecular
differences between golden henbane (Hyoscyamus aureus L) collected from
south Syria. Seeds were collected from six locations, then sterilized and grown
In Vitro. Grown plants we
re described for some morphological characters. The
molecular characterization was carried out using the method of Inter Simple
Sequence Repeats (ISSR) to detect genetic variations. The plants showed
significant (p<0.01) morphological differences among studied locations, Plant
height ranged from 14.97 to 18.97 cm and stem color ranged from red to green.
Trichome density also ranged between medium, high and very high.
Furthermore, significant differences in leaves dimensions were observed
according to the plant geographical location. The main root length ranged from
6.09 to 8.37 cm. Molecular analysis by ISSR produced a total of 56 bands, 49 of
which were polymorphic (87.5%), resulted from using 11 non-specific primers.
A genetic distance dendrogram was drawn. based on the results of genetic
characterization of plant from different locations. The plants collected from
Salkhad separated with a genetic distance of 0.312 while the plants collected
from other locations were grouped together, at the same time, they were
divided into two sub groups, the first group comprised plants collected from
Qalamoun and Dimas regions with a genetic distance of 0.032, whereas sub
group II content Bosra plants which separated clearly from those of Dara' dam
and Wadi Al-Zaidi by a genetic distance of 0.017, These results may indicate a
relationship between genetic characters of this plant species with geographic
distribution.
The antibacterial activity of Inula viscosa L. leaves extracts against some pathogenic bacteria, which were isolated from Al-Assad hospital laboratory in Lattakia, was tested by disc diffusion method.
Results showed that water extracts had antibacte
rial activity against all Gram positive and negative bacteria. The diameters of inhibition zones were between 9 mm against Pseudomonas aeroginosa and 41 mm against Proteus vulgaris.
All Gram positive bacteria showed sensitivity to all organic extracts, so they had antibacterial activity, while Gram negative bacteria showed resistance to chloroform extract except for the one of Klebsiella pneumoniae with an inhibition zone of 12 mm, and resistance for dichlormethan extract except for Pseudomonas aeruginosa with an inhibition zone of 15 mm. there was resistance for diethyl extract except for Serratia marcescens with an inhibition zone of 32 mm.
Ethyl acetate extract had antibacterial activity against all bacteria. Ethanol, methanol and acetone extracts showed antibacterial activity against all bacteria except for Escherichia coli with an inhibition zone of 10 mm.
This study indicated that water and organic Inula viscosa L. leaves extracts had antibacterial activity against pathogenic bacteria which also was tested, especially water extract, ethanol, methanol, ethyl acetate and hexane extracts. So the Inula viscosa L. leaves extracts may be sources for natural antibiotic in future.
The antimicrobial activity of aqueous extract of Zingiber Officinalis
were tested for their antifungal activity against the following
dermatophytes :Candida albicans, Cladosporium cladosporiosis ,
Cryptococcus neuphormans, Trichophyton violaceum a
nd against
other four bacteria : E. coli, Pseudomonas aeruginosa.
Staphylococcus aureus, Streptococcus pyogenes. The extract used in
traditional medicine for the treatment of nausea was tested in vitro
through the Agar Disk Diffusion Method. The minimum inhibitory
concentration (MIC) of extracts determined by the Agar dilution
method ranged from 1.5 to 12.5 mcg. The most sensitive
microorganisms to the extract were Candida albicans with MIC 1.5
and E coli with MIC 1.5 mcg.
In October 2014, the collected air-green samples of Basil herb were gathered from the region of MashtaAlhulu in the ,Then the volatile oil from fresh samples was extracted with N-Hexane and other samples with steam distilled water, and we have found
the percentage of the volatile oil in the first sample (N-Hexane) is 2,7% , and 2,5% in the second sample (steam distilled water). Suitable conditions were chosen to separate the components of the volatile oil by GC-MS and we noticed : - the first sample (extracted with N-hexane) contain 18 compounds, and they are: Six Terpenes compounds and they form 21.22%,Hydrocarbon compounds form 21.8% from the total weight and they are 7 compounds, and one compound from Ketone class dicycle and its percentage is 2.32%,and two Ester compound and they form 18.96%, One phenolic compound and forms 21.60%, one Ether compound and forms 14.08%. -the volatile oil from the second sample (extracted with steam distilled water) contains 11 compounds, and they are: 6 compounds belong to Hydrocarbon class and they are the main components in the volatile oil with a 91.24% of the oil total weight, One compoundfrom Ether class forms 1.62%, one phenolic compound which forms 3.93%, and three Terpenes compounds and they form 3.17%.
We aimed in this study to evaluate the effectiveness of Syrian propolis on some bacterial strains isolated from Al-Assad hospital in Latakia, to estimate its antimicrobial activity. Thus, we studied two propolis samples from two regions in the Syrian
coast, and prepared ethanolic extracts of propolis (EEP) with different concentrations (0.5%, 1%, 5%, 10%, and 20%). Then we tested these extracts on four strains of bacteria (Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, and Acinetobacter baumannii), using pore method on Muller Hinton Agar. As we observed, the bacteria showed diverse susceptibility to the extracts and the most susceptible bacterium was Staphylococcus aureus which was sensitive to all concentrations and especially for EEP 20%. However, the least affected bacteria were E. coli and Pseudomonas, as the 0.5% and 1% EEP had no efficacy. On the other hand, there were little differences in antimicrobial activity between the two samples of propolis.
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