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Influence of Some Home Preparation and Storage Conditions on Ascorbic Acid Content of Infant Formula

دراسة تأثير التخزين و التحضير المنزلي لحليب الرضع على محتواه من حمض الأسكوربيك

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 Publication date 2015
  fields Pharmacy
and research's language is العربية
 Created by Shamra Editor




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Vitamin C concentration was studied in samples of infant formula from 5 companies (referenced A-E) using the 2,6-Dichlorophenolindophenol titration (AOAC, 2006) method. Results revealed that ascorbic acid content in formulas D and C was the closest to its recommended daily intake (RDI) and to the concentrations which are mentioned on the label of the samples. Storage conditions at room temperature or in the refrigerator did not affect ascorbic acid levels. In comparison to vitamin C concentrations in milk dissolved in tap water, its levels decreased when water was warm (40°C) or hot (100°C), and its concentrations further declined in dissolved form kept for two hours after preparation.

References used
ALLEN, L.; DE BENOIST, B.; DARY, O.; HURRELL, R., Guidelines on food fortification with micronutrients.WHO and FAO, Switzerland, 2006, pages 43-91; 41 - 130
ANDERSON, R. H.; MAXWELL, D. L.; MULLEY, A. E.; FRITSCH, C. W., Effects of processing and storage on micronutrients in breakfast cereals. Food Technology, Vol. 30, 1976, pages 110-114
AOAC Official Method 967.21: Ascorbic Acid in Vitamin Preparations and Juices: 2,6-Dichloroindophenol Titrimetric Method. AOAC INTERNATIONAL 2006, 1968
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Sour orange (Citrus aurantium L.) seedlings were sprayed three times a season with four levels of urea (45 % N) (2.5, 5.0, 7.5 and 10.0 g/L) and two levels of ascorbic acid (150 and 300 g/L), each alone or in combinations. Meanwhile the seedlings of control treatment sprayed with distilled water during 2010 growing season, twenty days intervals between each spray and another. Results obtained at the middle of October of the same season, indicated that the foliar spray with urea at the concentrations of 5.0, 7.5 and 10.0 g/L significantly increased leaves N and chlorophyll concentrations, leaves number, seedling leaves area, seedlings height, main stem diameter and leaves fresh and dry weight, especially at the foliar spray with 10.0 g/L. The means of these parameters were 3.19%, 66.80 SPAD, 146.66 leaves/seedling, 3700.00 Cm², 83.66 Cm, 4.50 mm, 4.70 g, 1.53 g, respectively. Meanwhile ascorbic acid spray at two concentrations unsignificantly affected all parameters, except leaves chlorophyll content, leaves number and seedling leaves area. Most treatments of combinations spray with urea and ascorbic acid significantly affected all studied parameters, especially the treatment of foliar spray of urea and ascorbic acid at a concentration of 10.0 g/L and 300 mg/ L, which gave the highest means of leaves N concentration, leaves number, seedling leaves area and leaves fresh and dry weights, and it unsignificantly differs from the treatments which gave the highest means of seedlings height, main stem diameter and percentage of leaves dry matter weight .
The study was carried out on trifoliate orange rootstock trees (Poncirus trifoliata L.) at Ciano Research Station where the trees were sprayed with nutrients or gibberelic acid GA3 or togethers, beside the control (without spraying). Different trea tments of seed storage were tested in order to study their effect on seed viability to increase it. The seeds were stored for three months using four treatments: (a) seed storage in fruits at +4˚C, (b) Storage in juice at +4˚C, (c) Storage at lab temperature, and (d) seed storage at fridge temperature +4˚C. The results showed that all spraying treatments were positive at postharvest, as compared with the control. However, treatment of seed storage inside the fruit gave the highest significant germination rate for all spraying treatments. Treatments of spraying with nutrients were the best. The viability of seeds considerably decreased for all treatments of seed storage at fridge temperature +4˚C, whereas germination was zero for seed stored in juice and seed stored at lab temperature.
Pastrami, manufactured from beef and abdominal fat and of a ratio of 3 meat to 1 fat, was stored under refrigeration at (4±2 °C) and storage times (0, 15, 30, 60, 120, and 180 days). The effects of storage times on the most important chemical, micr obiological and sensory properties of pastrami were investigated. The results of chemical analyzes showed that the studied pastrami conformed to the Syrian Standards in terms of the proportion of salt and fat content, and it nearly conformed to the standards in terms of humidity. The results also showed a slight decrease in the percentage of moisture, fat and protein, and an increase in each of the acidity, proportion of total volatile nitrogen, soluble nitrogen and peroxide value in pastrami with the progress of the storage period at the temperature degree (4±2 °C). The microbial tests showed that pastrami is free from some sickening organisms especially salmonella and E. coli. It also showed that the census of the aerobic bacteria, yeasts and fungi was within the limits of the Syrian standards. The results showed deterioration in the microbial properties of stored pastrami with the progress of time; pastrami became invalid from a microbial point of consumption after two months of storage as a result of the big number of aerobic bacteria, yeasts and fungi. The sensory tests showed deterioration in the organoleptic characteristics of storage with the progress of time, the deterioration was clear after two months of storage; pastrami became unacceptable in terms of taste, color, smell and texture.
The aim of this research is to study the most important physical, chemical and microbial changes in several kinds of infant milk available in local markets. The milk samples were studied in conditions similar to those used by mothers when they prep are infant milk bottles at their houses. The results showed that moisture content increased in all samples from approximately 1% to 3.2-4.4% after storage for 9 days. Acidity increased considerably from 0.14% to 0.2%. The results showed that peroxide value passed the allowable limits (0.25) and reached about (0.47) in samples stored for 9 days at room temperature. However, the value for samples stored in the refrigerator ranged between (0.25-0.29). The free acidity number of fat for all samples specially the refrigerated ones was within the natural limits (<0.5). Chromatographic analyses showed apparent differences in fatty acid percentages between the kinds of milk. The rate of lenoleic acid (C 18-2 ) decreased during storage in all samples and in all conditions. Soluble nitrogen increased in all samples at the end of storage and the highest value was 0.49% in Nido milk samples. The solubility of milk samples was checked during storage and was found to decrease very little ( 1%). The total count of aerobic bacteria showed that all samples conform to the Syrian standards (No. 197, 1996) during the first six days of storage, but they passed the upper limits at the end of storage. However, all samples were free of pathogenic microbes .
Green tomato fruits of different studied hybrids such as, Amal, Steel, Tartila, and 2-75/96, which had been grown in a greenhouse at Abi- Jarash Farm were picked up and stored at temperatures of 12 c and 20 c for 29 days. The fruits, which were st ored at 12 c had been transferred to 9 cْ, and then stored for an additional period of two weeks. The chemical composition of the stored fruits was evaluated, which included: acidity percentage, total soluble solids, and the quantity of vitamine C. The ratio of fruit weight loss during storage was studied as well.
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