An HPLC separation of the geometric isomers of β-carotene was developed
to evaluate the Provitamin A content of Palm oil . Different stationary phases
were studied, the best separation of all-trans-, 9-cis- and 13-cis-β-carotene were
obtained with
calcium hydroxide columns using isocratic elution . Eluent was nhexane
or isooctane or mixtures of them. These techniques were used to
determine the provitamin A content of palm oil .
