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The research was executed in Biotechnology Lab (Faculty of Agriculture – Damascus University), during the season 2016. Sixteen genotypes of Sunflower Oil were planted to study the genetic diversity among these genotypes and to determine the degree of genetic Agreement using the technique SSR (Simple Sequence Repeats).
In this search we characterized 8 double purposes olive varieties (Mawi Istanbuli, Khilkhali Khishen, Dan, Mniekri, Doaibli, Khilkhali Saghir, Karamani Modabal, Sourani), which cultivated in ACSAD Gene bank in Gellin by ISSR molecular markers to determine the relationship between these varieties, we noticed that these varieties are so close to each others depending on quantitive traits, which ranged from 60% and 85%, it was noticed that Mneikri was apart from the other varieties in its traits, polymorphic rate was 92.94%.
The research aimed to evaluate the genetic diversity and the genetic relationship between 16 Hordeum vulgare genotypes (variaties and new lines), using SSR markers. 32 primer pairs were used in the analysis, five of them amplified primers monomorp hic alleles, while the remaining primers detected different alleles. A total of 126 polymorphic alleles were revealed by the 27 primer pairs. The number of different alleles detected on alocus ranged from 2 to 11with a mean of 4.66 alleles per locus. The polymorphic information content (PIC) values ranged from 0.21 to 0.88 with a mean value of 0.49, and the gene diversity ranged from 0.23 to 0.89 with a mean value of 0.53.The dendrogram was established using SSR data clustered into three major groups. The smallest genetic relationship was observed between line39 and line38 . The results proved the efficiency of SSR markers in the estimation of genetic diversity and in the clustering of the barley genotypes in Syria.
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