Pyocyanin was extracted from cultures of Pseudomonas
aeruginosa, which was isolated from sea water by chloroform.
Results study the effect of pyocyanin against human pathogenic
bacteria, which were isolated from Al-Assad hospital laboratory in
La
ttakia, with disk diffusion method showed that pyocyanin has
antibacterial activity against Gram- positive bacteria.
Four isolates of Streptomyces sp., St.1 and St.2 were isolated
from garden soil of science faculty, St.3 and St.4 were isolated
from Al-Shameia soil Lattakia, by serial dilution on Starch Casein
Agar medium.
The research was conducted with the aim of investigating in the
most important pathogens (coliform_ E.coli_ Salmonella), which
moves from irrigation water to many types of vegetables which
irrigated from it. And compared the extent of contaminatio
n by
irrigation source (treatment sewage, wells), and according to the
type of vegetable (fruit plants, tuberous vegetables), and the
irrigation method(surface irrigation, modern irrigation).
The antibacterial activity of Inula viscosa L. leaves extracts against some pathogenic bacteria, which were isolated from Al-Assad hospital laboratory in Lattakia, was tested by disc diffusion method.
Results showed that water extracts had antibacte
rial activity against all Gram positive and negative bacteria. The diameters of inhibition zones were between 9 mm against Pseudomonas aeroginosa and 41 mm against Proteus vulgaris.
All Gram positive bacteria showed sensitivity to all organic extracts, so they had antibacterial activity, while Gram negative bacteria showed resistance to chloroform extract except for the one of Klebsiella pneumoniae with an inhibition zone of 12 mm, and resistance for dichlormethan extract except for Pseudomonas aeruginosa with an inhibition zone of 15 mm. there was resistance for diethyl extract except for Serratia marcescens with an inhibition zone of 32 mm.
Ethyl acetate extract had antibacterial activity against all bacteria. Ethanol, methanol and acetone extracts showed antibacterial activity against all bacteria except for Escherichia coli with an inhibition zone of 10 mm.
This study indicated that water and organic Inula viscosa L. leaves extracts had antibacterial activity against pathogenic bacteria which also was tested, especially water extract, ethanol, methanol, ethyl acetate and hexane extracts. So the Inula viscosa L. leaves extracts may be sources for natural antibiotic in future.
