In this research, the concentration of thiocyanate ion in serum has been investigated,
thiocyanate has negative effects on human body when it increasses higher than specific
limit, this ion is produced by the metabolism of cyanide which exist in ci
garette smoke.
Spectrophotometric method has been used in the visible range at λ=460 nm, the formation
of the complex between thiocyanate and reagent Ferric chloride (FeCl3) have been made in
an ideal parameters such as temperature 25 ºC, time=15 min, pH=4.4, volume of citrate
buffer=0.75 ml, volume of reagent (FeCl3) =1 ml and concentration of reagent = 0.02
mg/ml.
The selected method showed good accuracy and precision, the accuracy of the method has
been validated with R=99.3%, and the precision of relative standard deviation was
(RSD<=1.149 % .
The concentration of thiocyanate has been studied in a sample of 240 non-smokers’ and in
a sample of 240 smokers’, these samples have been divided into different age groups.
The maximum concentration of thiocyanate was 89.49 μmol/L in the fourth group (>50
years) of the non-smokers’, and the maximum concentration of thiocyanate was 190.27
μmol/L in the fourth group (>50 years) of the smokers’. The maximum concentration of
thiocyanate was 91.647 μmol/L in the non-smokers’ males and 86.623 μmol/L for the nonsmokers’
females, whereas the concentration of thiocyanate was 204.53 μmol/L in the
male smokers’ and 167.33 μmol/L in the female smokers’.
Selenium is an essential nutritional mineral, and selenium deficiency diseases are known in medical literature. Studies have shown that selenium intake in high doses is toxic in humans. Selenium is widely distributed in nature and due to the importan
ce of selenium and its compounds in industrial applications; several analytical techniques have been reported to estimate selenium. In this study the optimal conditions for the formation of selenium complex with NEDA reagent will be studied and used for the control of selenium microcapsules, a method that is characterized by being rapid, sensitive and selective. The optical spectrophotometric method was used in the visible field at 540 nm wavelength to determine the complex between the selenium ion and the NEDA reagent under optimal conditions for complex formation namely temperature 50℃, time min t = 240, added reagent volume V=4ml, and reagent concentration 1%. This method showed good accuracy and correctness, as the method's validity was achieved through the relative percentage yield (R = 99.68%), while the accuracy of the method was achieved through the low value of the standard deviation (SD≤) (1.172) and thus the percentage relative standard deviation (RSD≤) (1.725). Selenium microcapsules were prepared, with an entrapment efficiency of 36% at a concentration of 3% for alginate and 1% while the entrapment efficiency rose to 51.2% at a concentration of 2% for calcium chloride.
