In this research two analytical methods were developed for the determination of Azelastin as raw material and in Nasal Spray formulation.
The first method was spectrophotometric while the other used the technic of HPLC The first method based on meas
uring the absorbance at λmax 284 nm .
The relationship between the absorbance and the concentration is rectilinear over the range 0.001 – 0.008 g %.
The proposed spectrophotometric method was successfully applied to the determination of Azelastin in nasal spray.
The percentage recoveries were 99.378±1 (10 duplicate readings)1
No interference was noticed from co-formulated drugs.
For the absorbance at λmax the obtained results were in good agreement with which obtained by the Pharmacopeia method.
The substance gave a good separation while the retention time was 1.467 min.
A new colorimetric analytical method was developed for determination of
Lisinopril in bulk and tablets .
The method is depended on derivatization chemical reaction between
Lisinopril and 4- nitrobenzene diazonium tetra fluoro borate in non
aqueou
s medium to give color derivatives with max. absorption at 293.8 nm
.
The resulting color was measured spectrophotometrically . The absorbance
concentration plots were recti linear over the range 5-50 μg /ml.
The method applied for determination the Lisinopril in Tablet, The
percentage recoveries was 98% ± 2.4.
The results obtained were in accordance with those found using the HPLC
standard method in Pharmacopoeia .
Terfenadine reacts with mixed anhydriedes (Malonic and acetic anhydrides)
producing a yellow-coulored product with intense fluorescence. Based on this
fact, a spectrophotometric method was developed for the determination of
terfendaine in dosage f
orms. The relation between the absorbance at 395 nm and
the concentration is rectilinear over the range 0.5-5 μg.ml-1. The reaction product
was also measured spectrofluorimetrically at 435 nm after excitation at 395 nm.
The fluorescence intensity was directly proportional to the concentration over the
range 0.5-4 ng.ml-1. The different parameters affecting the development and
stability of the reaction product were carefully studied and incorporated into the
procedure. The proposed spectrophotometric method was successfully applied to
the determination of terfenadine in tablets and suspensions; the % recoveries
were 99.83 ± 0.75 and 99.65 ± 0.83, respectively. The proposed
spectrofluorimetric method was applied to the determination of terfenadine in
spiked human plasma. the % recovery was 99.35 ± 2.19. The method is highly
sensitive and specific. No interference was noticed from co-formulated drugs,
such as pseudoephedrine and ibuprofen.
