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Register a new userDetermination of total content of flavonoids in dried and fresh hawthorn leaves and flowers
تحديد كمية الفلافونيدات الكلية في كل من أوراق و أزهار الزعرور البري الطازجة و المجففة
1986
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Tishreen University ورقة بحثية
Publication date
2017
fields
Chemistry
and research's language is
العربية
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Shamra Editor
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تهدف هذه الدراسة إلى تحديد المحتوى الكلي من الفلافونيدات في المستخلصات المائية، و الكحولية المائية لأوراق و أزهار الزعرور البري الجافة و الطازجة، إذ استخدمت طريقة النقع على البارد و الساخن لتحضير هذه المستخلصات و تم الكشف عن المركبات الفعالة الموجودة في هذه المستخلصات عن طريق إجراء تفاعلات كيميائية عدة. أظهرت نتائج هذه الدراسة احتواء المستخلصات على: االفينولات، الفلافونيدات، الصابونينات، أما الكمية الكلية من الفلافونيدات في المستخلصات فقد حددت بالطريقة الطيفية الضوئية في المجال المرئي عن طريق تشكيل معقد مع كلوريد الألمنيوم و قياس الامتصاصية عند طول موجة 510nm و تبين أن كمية الفلافونيدات في العينات المجففة تتراوح بين 0.16-1 % بينما كميتها في العينات الطازجة كانت بين 0.026-0.18%.
This study was carried out to determine the total content of flavonoids of dried and fresh hawthorn flowers and leaves in water and hydroalcolic extracts. The method of cold and hot maceration was used to prepare these extracts. The active compounds which were found in these extracts were detected by many chemical reactions. The results of these tests showed that the extracts contain: phenols, flavonoids, and saponins.The total content of flavonoids was determined using spectrophotometric methods by forming complex with aluminum chloride and measurement absorbance at 510nm, it has been shown that quantity of flavonoids in dried samples ranges from 0.16 to 1%, while it's quantity in fresh samples 0.026- 0.18%.
References used
KUMAR, D; ARIA, V; BHAT, Z. A; KHAN, N. A; PRASAD, D. N. The genus Crataeguse: chemical and pharmacological perspectives. Brazilian journal of pharmacognosy, ISSN 0102-695X, 2012, 14
MATOES, R. G. Antioxidant compounds in hawthorn fruits (Crataegus spp.) of Mexico. Revista Mexicana de biodiversidad, 84, 2013, 1298-1304
TASSEL,C.M; KINGSTON, R; GILROY, D; LEHANE, M; FUREY, A. Hawthorn (crataegus spp. ) in the treatment of cardiovascular disease. Pharmacogn Rev 4(7) , 2010, 32-41, 11
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Ten medicinal plants were chosen from various plants in Syria which
used as spices or cooked and Syria traditional medicine. Their names
Rosemary, Damask rose, Chamomile, Sumac, Silver linden, Black
cumin, Thyme, Mint, Laurel and lemon balm.
Mois
ture, yield of extraction, total phenolic content (TPC) and
antioxidant activities of methanol, ethanol, acetone and aqueous extracts
of medicinal plants were investigated.
The TPC were measured by Folin-Ciocalteu method, and the antioxidant
activity was assessed by the scavenging activity towards 2, 2′-diphenyl-
1-picryl hydrazyl (DPPH)-radical.
Total polyphenols (TP), total flavonoids (TF) and (-)-epigallocatechin gallate
((-)-EGCG) were determined in five commercial tea infusions by continuous
infusion method, using the ratio 20g of tea to 1L of water at 85±5 °C and
brewing time between 5-180 min.
The aim of this study was to estimate the total amount of phenolic compounds of olive
trees leaves (Olea europaea L.) as Biomarker for the assessment of lead air pollution at
Banias area. Olive trees were selected as the predominant species in the
study area. The
samples were collected from 6 locations at different distances from the vicinity of the
Banias refinery (0.1, 0.5, 2, 4, 6, 10) Km. The dominated wind in the region is western -
southwesterly during the winter (March) and summer (September), The control was taken
from an area about 20 km from the refinery to the north-east (al-Qardahah rural).
The concentration of total phenolic compounds (TPC) during the summer was (45.6-70.85)
mg GAE/g dw and in the control (40.4) mg GAE/g dw, and during the winter the content
of TPC was (35.6-52.9) mg GAE/g dw and in the control (25.8) mg GAE/g dw. The
concentration of Lead (Pb) in unwashed leaves during the summer was (0.879 – 2.170)
ppm and in the control (0.005) ppm, whilst during the winter was (0.479-1.023) ppm and
in the control (0.00083) ppm. The results showed significant differences in the
concentration of total phenolic compounds and lead between sites (Anova test). High
concentration of total phenols and lead in the summer compared to values in winter at all
sites (t-test). The results also showed a negative correlation between the concentration of
total phenolic compounds and lead with the distance from the refinery of Banias. Finally,
The results showed that the TPC can be used as biomarker of lead air pollution in Banias
area.
Total Polyphenol was studied in olive leaves collected from Lattakia area
(AlQurdaha zone), where Phenolic compounds were extracted from dry Olive leaves by
using two extraction methods: maceration and Ultrasonic Device. By the both two methods
a
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was done by using the mixture of Ethanol-Water with different percentages (60,70,80
%). As well, The extraction temperature effect was studied at the both methods at (20,30,
40°C); in addition to the study of the extraction time effect.
At the method of Maceration Extraction, the total polyphenols amount was studied in
dry olive leaves after extraction during different times (24,48,72 h). While at the Ultrasonic
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The study showed that the highest amount of phenolic compounds were existed in
dry olive leaves which were extracted by Ultrasonic Waves with the concentration of the
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Black tea which contains polyphenol is important for health benefits and it is
considered as antioxidant contents .
The extraction parameters, time and temperature and kind of solvent extraction, can
Influence the polyphenol concentrations of Blac
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epigallocatechin, epicatechin gallate, epigallocatechin gallate)
in different kinds of teas ( Lipton , Alrabee , Layalina ) which are available in Syria
market.The study was conducted using two types of solvents methanol 70% the adoption
of the reference method adopted ISO14502-1 and water 100% , at different extraction
times (1, 3, 5, and 10 minutes) and extraction temperatures (60°C , 70 °C , 80°C, 90°C,
100°C). the total polyphenols content was determined using the phenol reagent (Folin
Ciocalteu reagent) adoption gallic acid as standard compound
the overall content values of polyphenols have been monitoring in extracts methanol
70% and water 100% .
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