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Register a new userStability–indicating HPLC Assay Method of Aspartame
مقايسة الأسبارتام بطريقة الاستشراب السائل رفيع الإنجاز ذات دلالة لاختبارات الثبات
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محمد عامر المارديني( باحث )
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يهدف هذا العمل إلى تطوير طريقة استشراب سائل رفيع الإنجاز HPLC ،حساسة Sensitive ،انتقائية Selective و متحقق من صلاحيتها Validated و ذات دلالة ثبات indicating–Stability لمقايسة Assay أقراص الأسبارتام. جرى تحليل الأسبارتام بوجود شوائبه Impurities أو منتجات تدركه Degradation products و سواغاته Excipients على عمود C18 باستخدام طور متحرك يحوي محلول دارئة Buffer فسفات وحيدة الأساس بقيمة باهاء pH = 5.3 مع اسيتونتريل بنسبة 85/ 15.
The purpose of this work is to develop a sensitive, selective and validated stability–indicating HPLC assay for the analysis of aspartame tablets. Aspartame and its potential impurities or degradation products are analyzed on C18 Column using a mobile phase containing monobasic potassium phosphate buffer solution (pH = 3.5) and acetonitrile (85: 15).
References used
Coiffard,C; Coiffard,L-j-M and Roeck-Holtzhauer,YDE. Pharmazie 1998, 53: 251 – 253
The Merck index 12 th edition whitehouse Station. NJ 1996: 141 – 142
Peigne, F; Stojanovic, S; Couteau, C; Coiffard, LJM Sciences des aliments. 2000, 20: 523-525
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The present study describes a simple stability-indicating reversed-phase HPLC assay
for pentoxifylline in its pharmaceutical dosage forms. Separation of the drug and the
degradation products، under stress conditions was successfully achieved on a C
18 column
utilizing water: MeOH (60:40 v/v)، pumped at a flow rate of 1 ml min-1 with UV detection
at 272 nm. The retention time of pentoxifylline was about 14 min. The method was
satisfactorily validated with respect to linearity، precision، accuracy and selectivity. The
response was linear in the range of 0.6-3.5 μg/ml with R2 0.994. The method was accurate
(recovery 100.1%) and precise (RSD < 2%). Detection and quantification limit were 0.2
μg/ml and 0.4 μg/ml respectively. The suggested method was successfully applied for the
analysis of pentoxifylline in extended release tablets available in Syrian market.
Most epeliptic patients undergo treatment with the
anticonvulsive drugs, the most important of which is
phonation. The treatment may last for their life time. So
it should be insured to get the right theraputic dose to
reach and keep the optimal
phenytoin concentration in
the circulating body fluid (١٥ -٢٠ ug/ml). Althought
this patient should be under control in order to avoid
liver damage or the incidence of toxicity of over dose of
phenytoin (as a result of long term treatment
Phenol derivatives have been reacted with aryl halides under
heterogeneous basic catalyst (Amberlyst-21) using 5 mol% Cu(I)
Complex as catalyst, [CuClPPh3]4. The typical reaction has been
performed between p-cresol and bromobenzene. This reaction
is
achieved in o-xylene as solvent. However, the catalyst complex
does not dissolve in toluene rather it acts as heterogeneous catalyst.
Therefore, it is filtrated at the end of the reaction and reused several
times. Accordingly, new compounds were prepared by reacting one
of the bromoaryl derivatives with paracetamol. It is anticipated that
the synthesized compounds may have pharmaceutical application as
non-steroidial anti-inflammatory drugs (NSAIDs).
The Reaction of Glycerol carried out with tow aryl halides
under basic conditions (K2CO3) using 5 mol% of Pd(II)Complex as
catalyst, Pd(PPh3)2Cl2. The typical reaction has been performed
between glycerol and bromobenzene. This reaction is achieved
in
water as solvent. However, the catalyst complex does not dissolve
in aqueous solutions rather it acts as heterogeneous catalyst.
Therefore, it is filtrated at the end of the reaction and reused several
times. Accordingly, new compound were prepared by reacting
glycerol with 1-bromo-2,6-dichlorobenzene. It is anticipated that the
synthesized compounds may have pharmaceutical application.
This paper presents a developed HPLC method for the determination of
caffeine and theobromine in different black and green tea infusion samples,
using different infusion methods, such as infusion with and without boiling,
repeated and continuous i
nfusion. A mixture of double-distilled water and
methanol (60:40 v/v), was used as a mobile phase. In this condition, the
retention time for theobromine was three minutes and for caffeine five minutes.
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