عزل إنزيم galactosidase-β من دماغ الماعز حديثة الولادة باستخدام تسع طرائق، و قد وجد بأن
طريقة 2.0 مول/ لتر من خلات الصوديوم + 2.0 مول/ لترمن كلوريد الصوديوم PH 5 هـي الطريقـة
المثلى لاستخلاص الإنزيم و بأعلى فعالية نوعية له مقارنة ببقية الطرائق الأخرى التي استخدمت للغـرض
نفسه. و رسب هذا الإنزيم باتباع أربع طرائق و قد تبين أن استخدام الأسيتون البارد هي الطريقة الفُضلى.
و كان عدد مرات التنقية نحو 46.135 مرة و بلغت الحصيلة نحو 14.77 % عند استخدام الترشيح الهلامي
200-S Sephacryl) الخطوة الثانية). و بلغ الوزن الجزيئي للإنزيم 437.187 كيلو دالتون.
β-galactosidase enzyme was isolated from the new born goat brain by nine
methods, It was found that the sodium acetate 0.2 Mole/Liter +0.2Mole/Liter
NaCl PH5 method have given the highest specific activity of crude enzyme in
comparison with the other methods. Also, this enzyme was purified by using
four methods, the second one (cold acetone) was the butter. As a result the
purification fold was about 135.46 times and the yield about 77.14% by using
Sephacryl S200 (second step). This enzyme is 187.437 KDa as a molecular
weight.
References used
Abed, A. (2006). Isolation, Purification and Characterization of β-galactosidase From Local Chicken liver. Ph.D.thesis. University of Baghdad- Education College. Iraq
AL-Obaidy, H., 1975. Broad bean Lipoxygenase. M. Sc. Thesis, Food Sci. Dept. Agricultural college, Baghdad University
Al-Tamimi, S. (1996). The Enzymes Dissolving Proteins in Intact Grain, Abu Ghraib Variety, Infected with Sunna Insect and its Effect on Some Specific Characteristics. Ph.D. Thesis, College of Agriculture, Baghdad University
Some characteristics of β-galactosidase enzyme that was isolated from a new
born goat brain were studied. This study concluded that the enzyme is glucoenzyme
in which the carbohydrate part constitutes 22.1% in accordance with
phenol –sulfate acid
40 samples were collected from milk from Homs to isolate the
bacteria and study their ability to produce the enzyme β-
galactosidase and diagnose isolates the most efficient in the
production of the enzyme, the results showed the presence of 25
sporeforming isolates producing the enzyme β-galactosidase , the
effectiveness of β-galactosidase was estimated using
spectrophotometer at a wavelength of 420 nm.
B-D-Galactosidase (B-D- galactoside galactohydrolase , E. C. 3.2.1.23) , is one of the most important enzymes used in food processing . It a specific example of a glycosidase enzyme. Many organisms naturally synthesize B- galactosidase , including mi
The study included the selection of the best methods to extract the enzyme
among nine methods. The protein content was concentrated and precipitated
by cold acetone among other five methods of concentration (partial
purification). The purification
This study was carried out using white German goat race during 2000-2001
at the Research Center of Animal Production Division, Humboldt University,
Berlin, in order to examine the validity of the Lactocorder instrument, which
has been used for det