من أجل تقييم طريقة الوقاية المناعية بالاستشراب المنـاعي ICT و المعتمـدة علـى المستضـد
38Kda للمتطفرة الدرنية، جمعت العينات من ٢٤٥ شخصاً منهم ١١١ لديهم تدرن رئـوي و ٦٩
تدرناً خارج رئوي و ٦٥ شخصاً كمجموعة ضابطة (٢٥ مريضاً بأمراض رئوية غير التدرن، و ١٠
مرضى بأمراض غير رئوية و غير التدرن، و ٣٠ شخصاً سليماً). و قد وضع التشخيص بناء علـى
المعطيات السريرية و الأشعة و لطاخة القشع و التشريح المرضي. و أُجرِي لجميع العينات الفحـص
المصلي ICT و كانت النتائج كالتالي:
عند مرضى التدرن إيجابيي اللطاخة كان اختبار الـ ICT إيجابيـاً عنـد ٥٠ مـن ٥٥ مريضـاً
(الحساسية ٩١ (% و عند مرضى التـدرن الرئـوي سـلبيي اللطاخـة كانـت الحساسـية ٨٦ %
(٤٨/٥٦ ،( و بلغت حساسية الاختبار ٧٤ % عند مرضى التدرن خارج الرئوي (٥١/٦٩ . ( و كانـت
حساسية الاختبار عند مرضى التدرن بشكل عام ٨٣ % أما نوعية اختبار الـ ICDT فقد بلغـت
٩٦ % عند مرضى بأمراض رئوية غير التدرن، و ١٠٠ % عند الأشخاص الأصحاء.
To evaluate the rapid immuno-chromotographic assay ICT based on antigen 38
Kda of Mycobacterium tuberculosis, sera was collected from 245 persons, 111 of
them were pulmonary TB patients, 69 extrapulmonary, 25 having non TP
respiratory diseases, and 30 healthy control persons.
The diagnosis were based on usual testes such as signs and symptoms, X-ray,
smear and biopsy as well as the using of the serological test ICT the results were
as follows:
When pulmonary TB smear positive ICT was positive in 50 of 55 patients
(sensitivity 91%) whereas in pulmonary TB smear negative, sensitivity of ICT was
86 % (48/ 56). The sensitivity of the assay in extra-pulmonary TB patients was
74% (51/69) and the sensitivity of the test in all TB types was 83% (149/ 180) and
specificity of the test in nonTB pulmonary diseases were 96% and 100%in healthy
persons.
References used
Beige J, Lokies J, Schaberg T et al. Clinical evaluation of a Mycobacterium tuberculosis PCR assay. J Clin Microbiol 1995;33:90- 95
Bothamley G H, Rudd R, Festenstein F et al. Clinical value of the measurement of Mycobacterium tuberculosis specific antibody in pulmonary tuberculosis Thorax 1992;47:270-275
Bothamley GH. Serological diagnosis of tuberculosis. Eur Respir J Suppl 1995 Sep;20:676s-688s
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