هدف البحث إلى عزل بكتيريا السالمونيلا من الأجبان البيضاء المصنعة من حليب الغـنم بالطريقـة
التقليدية و تشخيصها بالاعتماد على نظام 20E API و تقنية PCR . من أجل ذلك أُحضرت 80 عينة مـن
الجبن الأبيض المصنع من حليب الغنم من مناطق مختلفة في سورية، أعطت 17 عينـة منهـا نتيجـة
إيجابية لوجود السالمونيلا فيها باستخدام تقنية الـ PCR ، و من ثم فإن نسبة العينات المخالفة للمواصفة
القياسية السورية هي 25.21 %, و بتحديد أنواع السالمونيلا المعزولة باستخدام نظام 20E API لوحظ
أن النوع السائد من السالمونيلا في هذه الأنواع من الأجبان هو arizona.S بنسبة 47 %, و بعده جـاء
%11.76 و بقـي % 17.6 بنـسبة S.paratyphi النوع و أخيراً % 23.61 بنسبة S.typhimurium
تابعاً للجنس دون تحديد النوع .spp Salmonella .و قد لوحظ أن عـدد العينـات الإيجابيـة لوجـود
السالمونيلا في الجبن البلدي أكبر بكثير من الجبن العكاوي، كما لوحظ أنه يمكن اسـتخدام تقنيـة الــ
PCR للكشف عن وجود السالمونيلا في الأجبان بشكل سريع و دقيق.
The objective of this investigation was the Identification of Salmonella spp.
isolated from white cheese (traditional processed from sheep milk) and classify
it by using API 20E and PCR techniques.
80 samples of white cheese (traditional processed from sheep milk) were
collected from different locations in Syria.
According to PCR technique, 17 samples gave positive result for salmonella
meaning that 21.25 % did not match the levels of the Syrian Standardization
and Metrology (S.S.M.).
According to API 20E technique, the dominat type of Salmonella was
Salmonella Arizona (47%), followed by Salmonella typhim (23.61 %),
Salmonella paratyphi (17.6 %) and Salmonella spp. (11.76 %).
Results showed that samples from local cheese positive for Salmonella were
much more than samples from Akkawi cheese.
The results showed that the PCR technique is fast and accurate and can be
used to Identify Salmonella in cheese.
References used
Beerens, H. and Luquet, F. M. 1987. “Guide Pratique Danalyse Microbilogique Des Laits Et Desproduits Laitiers“ Lavoisier -Paris
Bej A. K., Mahbubani M. H., Boyce M. J. and Atlas R. M. 1994. "Detection of Salmonella spp. in Oysters by PCR" Applied and Environmental Microbiology, 60(1):368-373
Bintsis, T. and Papademas, P. 2002. “microbiological quality of white brined cheeses” International Journal of Dairy Technology. 55 – 53
This study was conducted in the years 2008-2009-2010, it has analyzed for
Staph. aureus and isolated from 100 samples of traditional ice cream sold to
customers it has had collected from different regions parts of Damascus and his
countryside. The
This study aimed to investigate the possibility of rapid identification of
isolated bacteria Leuconostoc from Syrian white fresh cheese made of cow and
ewe milk using Fourier transform infrared spectroscopy (FTIR). 20 isolates (14
from ewe milk ch
In this paper, we evaluated some varieties of local white cheeses (Baladi and
Akkawi). 100 samples were collected randomly from various production sites in
Syria between 2003 and 2004. Chemical and microbiological analyses were
applied to determin
This investigation was conducted at the laboratory of Food Science
Department, Faculty of Agriculture, University of Damascus to determine the
effectiveness and the impact of using microwave radiations to reduce or
eliminate Salmonella typhi in co
This research aimed to identify the bacteriocin produced from a strain of
Strep. Thermophiles which were isolated from native Syrian white cheese and
to identify its characteristics.
The study showed that the produced bacteriocin is able to inhibi