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Register a new userStudy of the Anti Bacterial Effect of the Pericarps of Many Varieties of Punica Granatum on E.Coli Biotype (1) Which Reveal Resistance Toward Many Antibiotics
دراسة التأثير المضاد لقشر ضروب مختلفة لنبات الرمان في جراثيم الإشريكية القولونية النمط الحيوي (1) التي أبدت مقاومة للعديد من الصادات الحيوية
2014
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هدف هذا البحث إلى التحري عن الفعالية المضادة للجراثيم التي تبديها المستخلصات المحضرة من قشر ضروب مختلفة لنبات الرمان (من الفصيلة الرمانية) التي تنمو في سورية ضد جراثيم الإشريكية القولونية E.coli النمط الحيوي (1) و التي أبدت مقاومة للصادات الحيوية المدروسة.
This research aims to investigate the effectiveness of anti-bacteria shown by the extracts prepared from per carps of different types of Punica granatum ( L) ( from the family Punicaceae), which grow in Syria against Escherichia coli (E.coli) biotype (1), which were resistant to studied antibiotics.
Artificial intelligence review:
Research summary
تتناول هذه الدراسة تأثير المستخلصات المحضرة من قشور أنواع مختلفة من نبات الرمان على جراثيم الإشريكية القولونية النمط الحيوي (1) التي أبدت مقاومة للعديد من الصادات الحيوية. تم جمع 843 عينة حليب من أبقار مصابة بالتهاب الضرع في سوريا، وتم تحديد الجراثيم باستخدام منابت مختلفة واختبارات كيميائية حيوية. تم استخراج قشور الرمان باستخدام الماء، الكحول المطلق، والإيثر، وتم اختبار فعالية هذه المستخلصات ضد الجراثيم باستخدام طريقة الانتشار من الأقراص الورقية. أظهرت النتائج أن نسبة جراثيم الإشريكية القولونية النمط (1) كانت 33.35% من العينات، وأن المستخلصات المحضرة من قشور الرمان أظهرت فعالية مضادة لهذه الجراثيم، في حين لم تظهر الصادات الحيوية المدروسة أي تأثير مضاد. توصي الدراسة بإجراء بحوث إضافية لدراسة تأثير أنواع الرمان الأخرى على الجراثيم المقاومة للصادات الحيوية وتحديد المكونات الفعالة وتركيزاتها لإيجاد بدائل علاجية فعالة.
Critical review
دراسة نقدية: تقدم هذه الدراسة إسهامًا مهمًا في البحث عن بدائل طبيعية للصادات الحيوية التقليدية التي تواجه مشكلة مقاومة الجراثيم. ومع ذلك، هناك بعض النقاط التي يمكن تحسينها. أولاً، كان من الأفضل تضمين عدد أكبر من العينات لتأكيد النتائج بشكل أكبر. ثانيًا، لم يتم تحديد المكونات الفعالة في مستخلصات الرمان بشكل دقيق، مما يجعل من الصعب تحديد الآلية التي تعمل بها هذه المستخلصات. أخيرًا، كان من الممكن توسيع نطاق الدراسة لتشمل أنواعًا أخرى من الجراثيم المقاومة للصادات الحيوية لتقديم صورة أكثر شمولية.
Questions related to the research
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ما هي نسبة جراثيم الإشريكية القولونية النمط (1) من العينات الكلية؟
كانت نسبة جراثيم الإشريكية القولونية النمط (1) 33.35% من العينات الكلية.
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ما هي الطرق المستخدمة لاستخلاص قشور الرمان في الدراسة؟
تم استخلاص قشور الرمان باستخدام الماء، الكحول المطلق، والإيثر باستخدام جهاز سوكسلية وجهاز مبخر التخلية الدوار.
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هل أظهرت الصادات الحيوية المدروسة أي تأثير مضاد على الجراثيم؟
لم تظهر الصادات الحيوية المدروسة أي تأثير مضاد على الجراثيم.
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ما هي التوصيات التي قدمتها الدراسة؟
توصي الدراسة بإجراء بحوث إضافية لدراسة تأثير أنواع الرمان الأخرى على الجراثيم المقاومة للصادات الحيوية وتحديد المكونات الفعالة وتركيزاتها لإيجاد بدائل علاجية فعالة.
References used
Pradeep B.V, Manojbabu M.K, Palaniswam M. Antibacterial Activity of Punica granatum L. against Gastro Intestinal Tract Infection Causing Organisms. Ethnobotanical Leaflets 12: 1085-89. 2008
Nair R ، Chanda S .Antibacterial Activity Of Punica Granatum In Different Solvents. Indian Journal of Pharmaceutical science. Vol.67( 2) ، 239-243، 2005
Ahmet D, Ozgen M, Kenan S.Antimicrobial Activity of Six Pomegranate (Punica granatum L.) Varieties and Their Relation to Some of Their Pomological and Phytonutrient Characteristics. Molecules 2009، 14(5)، 1808- 1817
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Pathogenic germs have developed an increasing resistance toward many
antibiotics which form a true problem for human being ,because these germs can transport to him by
many ways such as water ,food and air that may cause many dangerous diseases to
human being.
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flowing plants: Olea europea Linn(Oleaceae) , Myrtus communis Linn(Liliaceae) ,Majorana syriacus
Linn (Laminaceae), Zingiber officinale Linn(Zingiberaceae),and Achillea falcata Linn(Asteraceae), against
Pseudomonas Aeruginosa.
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follows: 133 from urine , 37 swabs of wounds and burns,
10 swabs from the ear and pharynx, 12 from feces, 8
from CSF, 4 from blood. This s
tudy distinctly showed that
gram negative bacilli were the most isolates 136 (67%)
while 68 isolates (33%) were gram positive bacteria .
E. coli dominated (39.68%) of the total gram negative
bacilli.
This study distinctly showed an increase in the ratio of
E. coli resistance to one or more types of antibiotics , we
found that E. coli resistance to all antibiotics except
specific and expensive antibiotics, and we found that its
complete resistance to the second ,third and fourth
generation of cephalosporins , resistance to specific and
antibiotics such as Imipenem.
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The method of vegetative propagation by hard wood cutting is considered of the
most important ones of prevalent and successful in its cultivation to obtain a Punica
Granatum seeding which are genetically uniform.
In this research,it was used three
categories fPunica Granatum
,Municipal,French,Darcoche,where they were taken the hard wood cutting of sound trees
which free of insecticides and fungal infections and bacteria, and divided into groups
which in cluded five deals to know the effect of hormone,stratification,impact,the hard
wood cutting age and its length on rooting.
It was found that the last three tested categories had no hormone effects on rooting.
The soft wood cutting of one year-old overtook the ones of two-years of age,where
they have the largest effect of rooting.The highest precentage of rooting and stronger were
at the length of 30 cm.
Its advised for stratification of the Municipal class befor its cultivation because it
increases the rooting percentage.
Darcoche is easy for rooting, because it roots by ahigh percentage if it had a
stratification or impact by the conditions of continuous wetting before cultivation.
A total of 73 drinking water samples were collected from distribution
system in Lattakia city, the aim was to search for hetrotrophic bacteria and
investigate their resistance to chlorine and antibiotics. The antibiotic
susceptibility tests were:
carried out by using agar dillution method (MIC) and
the antibiotics tested were: Ampicillin, Chloramphenicol, tetracycline,
Amikacin, Ciprofloxacin, Cefotaxim, Gentamicin, Erythromycin Piperacilin/
Tazobactam, and cephalothin.
A sum of 133 bacterial strains were isolated and included by five genera:
pseudomonas, acinetobacter, flavobacter, chromobacter, and methylobacter.
The samples content of residual chlorine were ranged between 0.1 to 3.8 mg/l.
The majority of bacterial strains isolated were resistance to chlorine. The
multiple antibiotic resistances were common amongst isolated bacterial strains.
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