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Assaying Digoxin by Way of Enzyme Linked Immune Standardized Assaying (ELISA) in Digitalis Purpurea Which is Under the Effect of Salinity

معايرة الديجوكسين بطريقة المقايسة المناعية المرتبطة بالإنزيم (ELISA) في نبات الديجيتال الأرجواني Digitalis purpurea الخاضع لتأثير الملوحة

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 Publication date 2011
and research's language is العربية
 Created by Shamra Editor




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Background & Objective: The plant creates raw materials through primal metabolism in order to maintain crucial lively operations. While in the case that the plant is subjected to inhibition of growth as a result to environmental circumstances characterized by a high level of salinity, the Digitalis Purpurea used in this experiment changes its biological potential like bio-creation to form other necessary materials that take part in secondary metabolism operations, and hence affect the creation of glycosides that strengthen the heart, which Digoxin is one of.



References used
Nevena S.; Miroslava K. – Effect of salt stress on the growth and photosynthesis rate of bean plants (phaseolus vulgaris L.) Journal central European, 2008, 9: (3): 385-392
Richard N. Bennett and Roger M. Wallsgrove.- Secondary Metabolites in Plant Defence Mechanisms. New Phytologist, 1994, Vol. 127, No. 4, pp. 617-633
Roberts DM.; Buckley N - Antidotes for acute cardenolide (cardiac glycoside) poisoning.2006, Plain Language Summary
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The objective of this study is to compare the effect of five different Nacl concentration (0,50,100,150,200 mM) on seeds germination and seedling growth of two cultivars of soybean (sb-44,sb-172). Seeds were germinated in petri dishes with sterile filter paper moistened with 10 ml of test solution at 25 c˚ in dark . The obtained results showed that germination percentage , length of root and hypocotyl were decreased with the increase of the Nacl concentration in the medium. The best values was from cultivar sb-44 . After seven days of incubation , significant differences were observed between control and treated seeds . Germinated seeds were subcultured on basal Murashige and Skoog medium solid at the same Nacl concentration . The roots growth was mor affected by 200 mM Nacl compared to seedling growth . Plantlets were transfered into pots with nutrient soil , irrigated with saline water and were adapted at laboratory . we obtained good plants grew to maturity in 10- 12 weeks .
The objective of this study is to compare the effect of five different Nacl concentration (0,50,100,150,200 mM) on seeds germination and seedling growth of two cultivars of soybean (sb-44,sb-172). Seeds were germinated in petri dishes with sterile filter paper moistened with 10 ml of test solution at 25 c˚ in dark . The obtained results showed that germination percentage , length of root and hypocotyl were decreased with the increase of the Nacl concentration in the medium. The best values was from cultivar sb-44 . After seven days of incubation , significant differences were observed between control and treated seeds . Germinated seeds were subcultured on basal Murashige and Skoog medium solid at the same Nacl concentration . The roots growth was mor affected by 200 mM Nacl compared to seedling growth . Plantlets were transfered into pots with nutrient soil , irrigated with saline water and were adapted at laboratory . we obtained good plants grew to maturity in 10- 12 weeks .
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