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Register a new userAssaying Digoxin by Way of Enzyme Linked Immune Standardized Assaying (ELISA) in Digitalis Purpurea Which is Under the Effect of Salinity
معايرة الديجوكسين بطريقة المقايسة المناعية المرتبطة بالإنزيم (ELISA) في نبات الديجيتال الأرجواني Digitalis purpurea الخاضع لتأثير الملوحة
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عصام الشماع( باحث )
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خلفية البحث و هدفه: يقوم النبات باصطناع المواد الأولية من خلال عمليات الاستقلاب الأولي، و ذلك من أجل الاستمرار في العمليات الحياتية الضرورية. أما في الحالة التي يتعرض فيها النبات إلى تثبيط النمو نتيجة للظروف البيئية الشديدة مثل الملوحة فإن نبات الديجيتال الأرجواني المستخدم في هذه التجربة يغير من قدراته الحيوية مثل الاصطناع الحيوي من أجل تشكيل مواد أخرى ضرورية تدخل في عمليات الاستقلاب الثانوي، و من ثَم تؤثر في اصطناع الغليكوزيدات المقوية للقلب Cardenolides و منها الديجوكسين.
Background & Objective: The plant creates raw materials through primal metabolism in order to maintain crucial lively operations. While in the case that the plant is subjected to inhibition of growth as a result to environmental circumstances characterized by a high level of salinity, the Digitalis Purpurea used in this experiment changes its biological potential like bio-creation to form other necessary materials that take part in secondary metabolism operations, and hence affect the creation of glycosides that strengthen the heart, which Digoxin is one of.
References used
Nevena S.; Miroslava K. – Effect of salt stress on the growth and photosynthesis rate of bean plants (phaseolus vulgaris L.) Journal central European, 2008, 9: (3): 385-392
Richard N. Bennett and Roger M. Wallsgrove.- Secondary Metabolites in Plant Defence Mechanisms. New Phytologist, 1994, Vol. 127, No. 4, pp. 617-633
Roberts DM.; Buckley N - Antidotes for acute cardenolide (cardiac glycoside) poisoning.2006, Plain Language Summary
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The search has been performed on blood samples from cows with
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number of farms to detect sera-prevalence percentage of brucella
abortus infection in cows using Competitive Enzyme Linked
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mmune Sorbent Assay. The test results have shown general
serum spread by the percentage 5882%. The rate of antibodies of
serum spread in the cows in different farms under study have
been between 0.00% and 16.66%.
The incidence of contamination with ochratoxin A (OTA) in baby
food products consumed in Syria was investigated using the
competitive enzyme linked immunosorbent assay (ELISA)
technique.
The objective of this study is to compare the effect of five different Nacl concentration (0,50,100,150,200 mM) on seeds germination and seedling growth of two cultivars of soybean (sb-44,sb-172).
Seeds were germinated in petri dishes with sterile
filter paper moistened with 10 ml of test solution at 25 c˚ in dark .
The obtained results showed that germination percentage , length of root and hypocotyl were decreased with the increase of the Nacl concentration in the medium. The best values was from cultivar sb-44 .
After seven days of incubation , significant differences were observed between control and treated seeds .
Germinated seeds were subcultured on basal Murashige and Skoog medium solid at the same Nacl concentration . The roots growth was mor affected by 200 mM Nacl compared to seedling growth .
Plantlets were transfered into pots with nutrient soil , irrigated with saline water and were adapted at laboratory . we obtained good plants grew to maturity in 10- 12 weeks .
The objective of this study is to compare the effect of five different Nacl
concentration (0,50,100,150,200 mM) on seeds germination and seedling growth of two
cultivars of soybean (sb-44,sb-172).
Seeds were germinated in petri dishes with sterile
filter paper moistened with 10 ml
of test solution at 25 c˚ in dark .
The obtained results showed that germination percentage , length of root and
hypocotyl were decreased with the increase of the Nacl concentration in the medium. The
best values was from cultivar sb-44 .
After seven days of incubation , significant differences were observed between
control and treated seeds .
Germinated seeds were subcultured on basal Murashige and Skoog medium solid at
the same Nacl concentration . The roots growth was mor affected by 200 mM Nacl
compared to seedling growth .
Plantlets were transfered into pots with nutrient soil , irrigated with saline water and
were adapted at laboratory . we obtained good plants grew to maturity in 10- 12 weeks .
The effect of salinity and the treatment with fungicide on seed germination of Cupressus
sempervirens L. was investigated.
Five concentrations of salinity (0, 0.5, 1, 5 and 10%) were used. One part of the seeds was
treated with fungicides (Benomyl
), while the other part was germinated without fungicide
treatment. The results showed that seed germination was reduced by salinity, but the reduction was
more pronounced without fungicide and with salt concentration, the seeds didn't germinate at 5 and
10% NaCl.
Despite the decrease of germination indices with the increase of salt concentrations, the
statistical results didn't show significant differences in salinity concentrations and the control in
germination speed without fungicide, but they were significant with the fungicide. The statistical
results showed significant differences between the presence and absence of fungicide in all
treatments
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