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Register a new userThe Effect of Temperature Factor on the Detection of virus Caprine Arthritis- Encephalitis Virus (CAEV), using the technique of Real Time-PCR
تأثير عامل الحرارة في الكشف عن فيروس التهاب المفاصل و الدماغ الماعزي CAEV باستخدام تقنية RT-QPCR
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أنور العمر( باحث )
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تشكل هذه الدراسة مرحلة من مراحل تطويع تقنية تفاعل البوليميراز المتسلسل الكمي للكشف عن وجود فيروس التهاب المفاصل و الدماغ الماعزي Caprine Arthritis-Encephalitis Virus (CAEV في العينات الإكلينيكية في الحدود الدنيا الممكنة ضمن عينات السائل الطافي للمزارع الخلوية المصابة بالفيروس, و التي تعتمد على تحديد الجرعة الممرضة 50% لظهور الأضرار الخلوية المرضية على خلايا الزراعة الخلوية, و تحديد الحدود الدنيا لعدد الوحدات الحموية.
This study has been conducted to adapt a quantitative RT-QPCR to detect the least amount of virus and tissue culture pathogenic dose (TCID50) of CAEV showed cytopathogenic effect (CPE) from clinical samples.
References used
Fieni F., Pellerin J.L., Roux C., Poulin N., Baril G., Fatet A., Valasg S., Chatagnon G., Mermillod P., Guignot F. (2012) Can caprine arthritis encephalitis virus (CAEV) be transmitted by in vitro fertilization with experimentally infected sperm? Theriogenology 77. 644–651
Cheevers WP, Knowles DP, McGuire TC, Cunningham DR, Adams DS, Gorham JR. Chronic disease in goats orally infected with two isolates of the caprine arthritis-encephalitis lentivirus. Lab Invest 1988;58:510–7
Chebloune Y, Sheffer D, Karr BM, Stephens E, Narayan O. Restrictive type of replication of Ovine/caprine lentiviruses in ovine fibroblast cell cultures. Virology 1996;222:21–30
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Agricultural Research GCSAR, in cooperation with the PCR laboratory at the
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