This investigation was carried out at Tissue Culture Laboratory, Department
of Horticulture, University of Mosul, Iraq during 2012-2014) on Catharanthus
roseus Variety; Nirvana Pink Blush, which is one of the important medicinal
plant, containing
a group of alkaloids. Parts of leaves, nodes, and internodes were
cultured on MS medium supplemented with NAA for callus initiation, data were
taken after 28-day results refer that highest weight of callus; 0.612g, 0.639g ,0.835g
were obtained from cultivated parts of leaves, nodes and internodes on MS medium
supplemented with 1.0mg/l NAA respectively. Callus was initiated and cultured
using parts of leaves, nodes and internodes on MS medium supplemented with BA
and 1.0 mg/l of NAA, after 50 days.
Bone healing is a complicated biological mechanism that is affected by many hormonal, nutritional, and hemal factors. The term Biomechanical theory of bone healing is a concept that is created by us and is used for the first time to identify and stud
y the influence of mechanical rules on the bone tissue so we can study and imitate its mechanical response depending on the rules of balance and movement. We ensure that bone healing is just adaptation to auto bone forming mechanism in the surrounding mechanical site. The term is more comprehensive and accurate than the bio-compression term which was used before; and all of those are confirming the criteria to evaluate the efficacy of fixators used in orthopedic surgery.
The objective of this research isto study the in vitro effect of some plant hormones
(growth regulators) BAP,NAA, 2,4-D, and genotype on callus and bud formation from
embryonic axes of two Soybean cultivar seeds (sb-44, sb-172).
The embryonic axes
were cultured on MS basal medium and MS supplemented with
1, 2, 3 mg/l BAP alone and in combination with 0.5 mg/l NAA.The cultures were
maintained at 25 C°±1 with photoperiod of 16 hours light (2000-2500 lux) and 8 hours
dark.The highest percentage92.5% and mean average 4.63 of callus formation were
recorded on MS medium containing BAP (3 mg/l) and NAA (0.5 mg/l).
The highest percentage 67.5% and average 3.38 of buds formation were obtained on
MS medium supplemented 1 mg/l BAP and 0.5 mg/l NAA in cultivar sb-44.
The percentage of callus formation increased, while the percentage of bud formation
decreased with each increase in BAP concentration when used alone. A positive increase
was observed in all mediums in the combination of 0.5 mg/l NAA or 0.5 mg/l 2. 4-D in the
two cultivars used in this study.
This study showed the genotypic difference effect on callus and bud formation.Roots
were formed from allplantlets cultured on MS medium without plant hormones.Rooted
plantlets were transferred into pots with nutrient soil, irrigated with water, and adapted
tolaboratory conditions. Good plants grown to maturity were obtained in 12-13 weeks.
This Research was conducted in the laboratory of tissue culture affiliated to
the National Commission of Biotechnology (NCBT) during the period between
2011 -2012 for induction of callus from mature embryos for three local
Genotypes of grain Sorgh
um, regeneration of the plant from callus, rooting
plantlets and acclimatization in order to get a plant capable to grow in
greenhouse. The best concentration for sterilizing the plant and entering it in
use was 5% NaOCL for 20 minutes. The addition of 2 mg/l of 2,4-D (2,4-
dichlorophenoxyacetic acid) to MS medium caused an increase in the ratio of
callus induction and embryogenic callus.
Stages of initial callus and embryiods of date palm Phoenix dactylifera L.
were histological and chronological studied begging from the ٢nd weeks of
culturing segment of shoot tip to ten months in MS medium containing ١٠٠mg/l
٢،٤-D with activated
charcoal in the dark. The study reveled that the active
meristematic cells were unequally distributed in the explant and concentrated
in the shoot apical dome and leaf margin. These cells are the bases from which
initial callus started its growth. Somatic proembryo appeared for the first time
as a meristematic cells groups separated from each other by relatively thick
walls after five months of culturing in continuos culture in the same medium .In
progress months (٦-١٠) the small simple proembryos continued into advanced
multicell embryos and underwent polarization. Also the embryos were
observed with attendance for division and separation into smaller or budded to
produce numerous other.
