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This research deals with amain steps related to preparing live food which is necessary to grow up larvae of different species of marine family Penaeidae prawn, in this study the isolation of two species of phytoplankton one cells they are Chlorell a sp, Tetraselmis sp, and the specimens are collected from littoral of jableh and rearing them up in hard and liquid medium in laboratory in Faculty of Sciences, Tishreen University, in the period between April and august 2013.
This researchwas conducted for the first time in Syria in order to studytheeffect of eyestalk ablation on moulting and ovarian maturation in PenaeusSemisulcatusas on essential step in most shrimp hatcheries . 12 females 15,5-17,5 cm and 6 adult males with a length of 14-16 cm were colectedat the sublittoal zone of city Jableh during the period from the beginning of the month of April 2012 until the end of June 2013. Eyestalks for 6 females were ablated while other females were left as control, and females were gathered with males by 2 : 1 in 150 L- tank connected to the basin 's filtering, controlled temperature range between 27-29 co and 38 ‰ salinity and oxygen dissolved 4 mg / l , Animals were fed individual once daily at a rate of 12 % of body weight with stuffed squid and shrimp. the study showed that all femaleswith eyestalk ablation were molted during the period of 2-6 days, the ovaries of five females began activeand one of female died, while females withnon of eyestalk ablation over a period of 8-20 days and ovaries oftwoof them showed development.
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