Our study aimed to evaluating significance of survivin as a
prognostic marker of relapse in children with acute lymphoblastic
leukemia. The study included 46 child (30 males, 16 females): 24
child with ALL at 35 day from BFM 95 Protocol and 22 rel
apse
child with ALL.
يعد الابيضاض النقوي الحاد أحد أكثر انواع الابيضاضات شيوعاً لدى البالغين وقد ازدادت معدلات الإصابة به والوفيات الناتجة عنه بشكل ملحوظ في السنوات الاخيرة
Gene regulation by microRNAs (miRNAs) is one of the most important regulatory
networks which control normal hematopoiesis. Disturbances in miRNAs levels lead to
proliferation diseases including leukemogenesis. MicroRNAs is a major topic of many
ca
ncer researches performed to discover noninvasive biomarkers used for diagnosis,
prognosis, and optimization of clinical decision.
This study, the first to be performed in Syria, aimed at monitoring miR-155 levels
compared to a normalizer gene RNU6-2 by quantitative reverse transcriptase -PCR (qRTPCR)
in a sample of newly diagnosed untreated AML patients at several hospitals in
Damascus in comparison with healthy controls. Changes in miR-155 gene expression
levels were calculated in patients and controls using the 2-ΔCt method.
The most important finding was the association of low and very high miR-155 levels
with poor prognosis reflected in failure to accomplish complete remission and high
mortality. In addition, high miR-155 levels were associated with M4 AML subtype,
although with large variance among patients. We hope our preliminary study pave the road
for many future research studies related to the applicability of microRNAs in supporting
diagnosis, predicting prognosis, and enhancing the personalized therapies which deal with
patients as individual cases.
In contrast to adaptive immunity and to certain components of innate immunity, only little is known on the influence of the complement system on cancer suppression and promotion . The lectin pathway of complement activation is an important part of in
nate immunity. It is mainly involved in opsonisation and lysis of pathogens and in recruitment of inflammatory cells.
Our study aimed to assess the relationship between MASP-2 serum levels before and after chemotherapy in Syrian children with acute lymphoblastic leukemia, aiming to find a relation, which serve in early forecast of patients response to chemotherapy, where the study has been done at Children University Hospital in damascus between 2013-2014. This study included 37 children diagnosed with acute lymphoblastic leukemia (ALL). The levels of Mannose-Binding Lectin-associated Serine Protease-2 (MASP-2) were determined by Enzyme Linked Immuno Sorbent Assay (ELISA).
The result was that serum levels of MASP-2 was strongly reduced after chemotherapy in children with acute lymphoblastic leukemia, and a strong correlation between serum marker levels before and after chemotherapy was found.
Chemotherapy causes neutropenia and increase susceptibility to infections. Morbidity and mortality due to infections remain serious problem in children with cancer. Decreased serum levels of Mannose-binding lectin ( MBL) may represent a risk factor f
or infections in children with acute lymphoblastic leukemia receiving chemotherapy.
The aim of this study was to determine whether low MBL levels is associated with increased risk of infections in Syrian children with acute lymphoblastic leukemia.
The study has been done at Children University Hospital in Damascus between 2013-2014. This study included 37 children diagnosed with acute lymphoblastic leukemia (ALL) who were candidates for chemotherapy, and the infections were identified depending on patients files. The levels of Mannose-Binding Lectin (MBL) was determined by Enzyme Linked Immuno Sorbent Assay (ELISA).
The result was that MBL serum levels are higher in patients who did not develop infections compared to patients who developed infections, and all patients with low MBL levels developed infections in contrast to patients with normal MBL serum levels.
Flow cytometry became unavoidable step in the diagnosis of
Acute Leukemia . However, accurate diagnosis is conditioned by using
proper markers. Objective: to evaluate the quality of performance of four
colors protocol, we used for immunophenotyping of acute leukemia in
children.
The goal of this research is to study the expression of epidermal and
transforming growth factor receptor in normal human bone marrow (BM)
cell, and in leukemia ( myeloid and lymphatic leukemia ) which promoted
the research for the function of EGF
R receptors in the development and
growth of BM cells and for malignancies expressing of the EGFR as
potential therapeutic targets in blood cell malignant diseases.
