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Register a new userEvaluation of a Four Color Protocol for Diagnosis of Pediatrics Acute Leukemia
تقييم الأهمية التشخيصية لمجموعة من المستضدات الخلوية في تشخيص ابيضاض الدم الحاد عند الأطفال
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Damascus University ورقة بحثية
Publication date
2009
fields
Medicine
and research's language is
العربية
Authors
إلهام حرفوش( باحث )
Created by
Shamra Editor
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يؤدي التنميط المناعي للخلايا دوراً مهماً في تشخيص آفات الدم الخبيثة. على أن التشخيص الدقيق للحالة الابيضاضية يعتمد على الاختيار الملائم لأضداد المستضدات السطحية. الهدف: تقييم الأهمية التشخيصية لمجموعة من المستضدات الخلوية السطحية و السيتوبلاسمية في ابيضاضات الدم الحادة عند الأطفال.
Flow cytometry became unavoidable step in the diagnosis of Acute Leukemia . However, accurate diagnosis is conditioned by using proper markers. Objective: to evaluate the quality of performance of four colors protocol, we used for immunophenotyping of acute leukemia in children.
Artificial intelligence review:
Research summary
تتناول هذه الدراسة تقييم الأهمية التشخيصية لمجموعة من المستضدات الخلوية في تشخيص ابيضاض الدم الحاد عند الأطفال. تم إجراء الدراسة على 651 حالة من الأطفال المقبولين في مشفى الأطفال الجامعي بدمشق خلال الفترة من 2005 إلى 2009. استخدمت تقنية الجريان الخلوي باستخدام أضداد وحيدة النسيلة لتحديد مجموعة من المستضدات الخلوية. أظهرت النتائج أن المستضدات TdT, CD3 و MPO أبدت نوعية مطلقة لنمط ابيضاضي معين، بينما أظهرت المستضدات الأخرى مثل CD2, CD7, CD13, CD15, CD33, CD117, CD79a, DR تعبيرًا شاذًا في خط خلوي مخالف. ومع ذلك، كانت معظم هذه المستضدات تلعب دورًا مهمًا في التشخيص النهائي للحالة. أظهر المستضد CD34 أهمية كبيرة في تشخيص الابيضاض الحاد، ولكنه كان محدودًا بنسبة 60% من الحالات. توصلت الدراسة إلى أن استخدام مجموعة من المستضدات بما في ذلك CD3, MPO, TdT, CD34, CD7, CD117, CD33 هو أمر ضروري للحصول على تشخيص دقيق لابيضاضات الدم الحادة عند الأطفال.
Critical review
تقدم الدراسة مساهمة مهمة في مجال تشخيص ابيضاض الدم الحاد عند الأطفال من خلال تحليل دقيق لمجموعة من المستضدات الخلوية. ومع ذلك، يمكن الإشارة إلى بعض النقاط التي قد تحتاج إلى تحسين. أولاً، الدراسة تعتمد على مجموعة محددة من المستضدات، وقد يكون من المفيد توسيع نطاق البحث ليشمل مستضدات أخرى قد تكون ذات أهمية تشخيصية. ثانيًا، الدراسة تركز بشكل كبير على التحليل الكمي للمستضدات دون النظر بشكل كافٍ إلى السياق السريري لكل حالة، مما قد يؤثر على دقة التشخيص. أخيرًا، الدراسة لم تتناول بشكل كافٍ التكلفة الاقتصادية لاستخدام هذه المستضدات، وهو جانب مهم يجب مراعاته خاصة في البلدان ذات الموارد المحدودة.
Questions related to the research
-
ما هي المستضدات التي أظهرت نوعية مطلقة لنمط ابيضاضي معين؟
المستضدات التي أظهرت نوعية مطلقة لنمط ابيضاضي معين هي TdT, CD3 و MPO.
-
ما هي نسبة الحالات التي أظهر فيها المستضد CD34 أهمية في تشخيص الابيضاض الحاد؟
المستضد CD34 أظهر أهمية في تشخيص الابيضاض الحاد بنسبة 60% من الحالات.
-
ما هي الفترة الزمنية التي أجريت فيها الدراسة؟
أجريت الدراسة خلال الفترة من 2005 إلى 2009.
-
ما هي التقنية المستخدمة في الدراسة لتحديد المستضدات الخلوية؟
التقنية المستخدمة في الدراسة لتحديد المستضدات الخلوية هي تقنية الجريان الخلوي باستخدام أضداد وحيدة النسيلة.
References used
Einsiedel HG et al,.Long-term outcome in children with relapsed ALL by risk-stratified salvage therapy: results of trial acute lymphoblastic leukemia-relapse. J Clin Oncol. 2008 May 1;26(13):2238
Winter, S. et al., Identification of genomic classifiers that distinguish induction failure in T-lineage acute lymphoblastic leukemia: a report from the Children's Oncology Group. Blood . 2007, 110: 1429
Pui CH, et al, Childhood and adolescent lymphoid and myeloid leukemia. Hematology Am Soc Hematol Educ Program. 2004:118-45
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