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Register a new userStudy of the Main components of the organic matter extracted from the leaves of Syrian Melilotus Officinal plant using GCMS and their effects on some of Pathogenic Microorganisms
دراسة المركبات الكيميائية العضوية لمستخلص أوراق نبات الحندقوق (Melilotus Officinal) باستخدام تقانة GC- MS و تأثيره على بعض الأحياء الدقيقة المرضية
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Tishreen University ورقة بحثية
Publication date
2018
fields
Chemistry
and research's language is
العربية
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Shamra Editor
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نظراً لأهمية نبات الحندقوق من الناحية الإقتصادية و الطبية, و لانتشاره الواسع في البيئة السورية , فقد شكل ذلك أساساً للقيام بدراسة أولية للمكونات الأساسية للزيت العطري المستخلص من أوراق هذا النبات. تم استخلاص الزيت العطري و تنقيته, و من ثم درست المستخلصات باستخدام جهاز (الكروماتوغرافيا الغازية كاشف مطيافية الكتلة GC-Mass spectrometry). و استخلص الزيت العطري بالميتانول باستخدام جهاز سوكسيليه , و طريقة النقع بالهكسان.
Due to the economical and medical importance of the Melilotus plant and the wide distribution of this plant in the Syria environment, it was of necessity to study the the composition of organic matter extracted from the leaves of this plant. The Melilotus Officinal organic matter was extracted and purified their where were studied by GC-Maas spectrometry. The organic matter was obtained by Soxhlet extractor and by solvent n-Hexane. Components have been found which was about (99.91%) from the total of Essential Oil. The major components were extracted by Soxhlet extractor and the following components were determined : n-Docosane(39.82%) , Hydrocoumarin(15.39%) , Methyl 3-(2-hydroxyphenyl)propionate(14.29%). The major components were determined by solvent n-Hexane: 1,2-Benzenedicarboxylic acid(17.77%) , 9,12,15-octadecatrienoic acid(12.85%) The effects of the extracted aromatic oil from the leaves of Melilotus plant on some of Pathogenic Microorganisms were studied.
References used
SCHEUER, M. L.; PDLEY, T. A."The evaluation and treatment of seizures". The New England Journal of Medicine.323(21),1990,pp:74-1468
NASSER, N. M.; AL-ANI, W."Isolation of coumarin from Melilotus Officinalis of Iraq". Pharmacie Globale ,2(6),2014,pp:1
NASSER, N. M.; AL-ARAJI, M.; AL-ANI, M. K."Antifungal activity of Melilotus Officinalis of Iraq".Journal of Chemical and Pharmaceutical Research,6(11),2014,pp:611-617
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Due to the economical and medical importance of the Capparisspinosa plant and the
wide distribution of this plant in the Syria environment, it was thought that a study of the
composition of Essential Oil extracted from this plant.
The Capparisspin
osa Essential Oil was extracted and purified components wher
studied by GC-Mass spectrometry.
The Oil was obtained by steam distillation (Clevegerexractor) and by solvent n-
Hexane. Components have been found which was about (98.9 %) from the total of
essential oil.
The major components were determined by steam distillation which were the
following:Palmitic acid (21.12%), Hexatriacontane (15.87%), n-Heneicosane (10.96%),
Pentatriacontane (9.92%), Hexahydrofarnesyl acetone (8.51%).
The major components were determined by solvent Hexane: Isobornyl acrylate
(66.89%), 2-Azido-2,3,3-trimethylbutane (6.09%), Ethanolamine (5.41%).
Bioeffect of some Syrian Myrtus communis L. leaves extracts in growth of some pathogenic microorganisms, which were isolated from Al-Assad hospital laboratory in Lattakia, was tested by disc diffusion method. Results showed that cold and hot water ex
tracts have antibacterial activity against all Gram positive and negative bacteria, and pathogenic Candida albicans except Klebsiella pneumoniae. All organic extracts have antibacterial activity against Proteus vulgaris, Entrococcus faecium, Staphylococcus aureus and Staphylococcus albus.
Pseudomonas aeruginosa and Pseudomonas fluorescens were affected by most of the organic extracts, and the most bioactivity was by methanol extract, and all inhibition zones of extracts were bigger than inhibition zones by control
antibiotics. Serratia marcescens and Acinetobacter calcoaceticus were affected by methanol (with highest inhibition zone 37.33 and 27.33 mm respectively), ethanol, acetone and ethyl acetate extracts, but resistant to the others. Klebsiella pneumoniae affected by ethanol extract only with inhibition zone 20.33 mm.
Results of bioactivity according to a various solvents used in this study demonstrate that Syrian Myrtus communis L. leaves extracts have antimicrobial activity, so expected to be potential sources of natural antibacterial and antifungal products against some pathogenic bacteria and fungi in the future.
Phenols were studied in the leaves of Syrian olive varieties collected from (Harasta
and Ghouta, Damascus Countryside and Qalamun). Phenolic compounds were extracted
from the olive leaf varieties after disposal of plant pigments and fatty compounds, and
total phenols separated with solvent (methanol - water).
In field's experiment had been done in the Tartus governorate during the growing season (2013-2012) Different rates of organic fertilizers were used, the experimental transactions were (10) treatments, control (without fertilization) sheep wastes(10-
20-30)tn/h, cow wastes(10-20-30)tn/h, poultry wastes(10-20-30)tn/h.
This study aimed to know the effect of adding these fertilizers on
biological properties of the soil (the number of physiological groups of microorganisms, and soil respiration).
The Results showed increasing in the number of Hertotrophic
bacteria , Actenomycetes in the soils which were fertilized in
poultry wastes(20),(30)tn/h, and increasing in the number
of(bacteria, using mineral from nitrogen, and decomposed of
celluose micro organisms) in the soils which were fertilized in
poultry wastes(30)tn/h and cow wastes(30)tn/h.
As for fertilization, The Results showed decreasing pH in the soils
which were fertilized cow wastes(30)tn/h, while soil contents of
available P and K and organic mater increased in soil which were
treated fertilized in poultry wastes(20),(30)tn/ h, and cow
wastes(30)tn/h.
The extracted oil from Gundelia Tournefortii seeds, which belongs to Asteraceae
family and which is known as one of the medical herbal plants that is widely spread in the
Syrian environment, was isolated using soxhlet continuous extraction. It's ch
emical
composition was determined by GC/MS (Gas Chromatography – Mass Spectrometry). The
analysis led to identification of 20 components. The extracted oil was characterized by
high content of Linoleic Acid (52.9%), Palmitic Acid (20.1%) and Squalene (10.1%).
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