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Study of The Inhibition Effect of Yoghurt Starter on Salmonella typhi O9 Bacteria

دراسة الأثر التثبيطي لبكتريا بادئ اليوغورت إزاء نمو بكتيريا Salmonella typhi O9

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 Publication date 2011
and research's language is العربية
 Created by Shamra Editor




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This investigation was done in the laboratories of Faculty of Agriculture – Damascus University Department of Food Sciences. The aim of this research was to study the inhibition effect of the growth of Lactic acid bacteria used as starter, on the growth of S. typhi O9 bacteria. We used for this study a pure strains of Salmonella typhi O9 and Lactic acid bacteria (Streptococcus thermophilus and Lactobacillus bulgaricus). The effect of Lactic acid bacteria on the number of S. typhi O9 was studied in 37 ◦C and 4◦C. The Lactic acid bacteria were inoculeted in milk for the yoghurt manufacturing. The number of S. typhi O9 decreased gradually with time. This decrease number of this bacteria was more clear when both Str. thermophilus and Lb. bulgaricus were used as a mixted starter, Lb. bulgaricus then when was used a single starter, or when Str. thermophilus was used a alone. The S.typhi O9 disappear


Artificial intelligence review:
Research summary
تتناول هذه الدراسة التي أُجريت في مختبرات كلية الزراعة بجامعة دمشق تأثير بكتيريا حمض اللاكتيك المستخدمة كبادئ في تصنيع اللبن على نمو بكتيريا Salmonella Typhi O9. استخدمت الدراسة سلالات نقية من بكتيريا Salmonella Typhi O9 وبكتيريا حمض اللاكتيك (Streptococcus thermophilus وLactobacillus bulgaricus). تم دراسة تأثير بكتيريا حمض اللاكتيك على عدد بكتيريا S. Typhi O9 عند درجات حرارة 37 درجة مئوية و4 درجات مئوية. أظهرت النتائج أن عدد بكتيريا S. Typhi O9 انخفض تدريجيًا مع مرور الوقت، وكان هذا الانخفاض أكثر وضوحًا عند استخدام مزيج من Streptococcus thermophilus وLactobacillus bulgaricus مقارنة باستخدام كل نوع على حدة. اختفت بكتيريا S. Typhi O9 بعد 12 ساعة من التخزين عند 4 درجات مئوية، بينما اختفت بعد 24 ساعة من التخزين عند 37 درجة مئوية.
Critical review
دراسة نقدية: تقدم هذه الدراسة معلومات قيمة حول تأثير بكتيريا حمض اللاكتيك على نمو بكتيريا Salmonella Typhi O9، وتساهم في فهم كيفية استخدام هذه البكتيريا في تصنيع منتجات الألبان للحد من نمو البكتيريا الضارة. ومع ذلك، يمكن تحسين الدراسة من خلال توسيع نطاق التجارب لتشمل أنواعًا أخرى من البكتيريا الضارة وتقييم تأثير بكتيريا حمض اللاكتيك عليها. كما أن الدراسة لم تتناول تأثير العوامل البيئية الأخرى مثل درجة الحموضة والرطوبة على فعالية بكتيريا حمض اللاكتيك، وهو ما يمكن أن يكون موضوعًا لدراسات مستقبلية.
Questions related to the research
  1. ما هو الهدف الرئيسي من هذه الدراسة؟

    الهدف الرئيسي من الدراسة هو دراسة تأثير بكتيريا حمض اللاكتيك المستخدمة كبادئ في تصنيع اللبن على نمو بكتيريا Salmonella Typhi O9.

  2. ما هي البكتيريا المستخدمة في هذه الدراسة؟

    استخدمت الدراسة سلالات نقية من بكتيريا Salmonella Typhi O9 وبكتيريا حمض اللاكتيك (Streptococcus thermophilus وLactobacillus bulgaricus).

  3. ما هي النتائج الرئيسية التي توصلت إليها الدراسة؟

    توصلت الدراسة إلى أن عدد بكتيريا Salmonella Typhi O9 انخفض تدريجيًا مع مرور الوقت، وكان هذا الانخفاض أكثر وضوحًا عند استخدام مزيج من Streptococcus thermophilus وLactobacillus bulgaricus مقارنة باستخدام كل نوع على حدة. اختفت بكتيريا S. Typhi O9 بعد 12 ساعة من التخزين عند 4 درجات مئوية، بينما اختفت بعد 24 ساعة من التخزين عند 37 درجة مئوية.

  4. ما هي التوصيات التي يمكن استخلاصها من هذه الدراسة؟

    توصي الدراسة باستخدام مزيج من بكتيريا Streptococcus thermophilus وLactobacillus bulgaricus في تصنيع منتجات الألبان للحد من نمو بكتيريا Salmonella Typhi O9، ويمكن توسيع نطاق البحث ليشمل أنواعًا أخرى من البكتيريا الضارة وتقييم تأثير العوامل البيئية المختلفة.


References used
Holcomb, D. L.; Smith, M. A.; Ware, G. O.; Hung, Y. C.; Brackett, R. E.; Doyle, M. P. (1999). Comparison of six dose-response models for use with food-borne pathogens. Risk Anal., 19: 1091-1100
Issa, M. S. and Ryser, E. T. (2000). “Fate of Listeria monocytogenes, Salmonella typhimurum DT 104 and Escherichia coli O157:H7 in Labneh and Postferment Ation Contaninant“ Int. of Food Protection. 63 (5): 608-612
Jay, J. M. (1996). “Modern food Microbiology – fifth edition“ Chapman and Hall New- York. 372-384
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This investigation was done in the laboratories of the Faculty of Agriculture – Damascus University in the Department of Food Sciences. The aim of this research use to study the inhibition effect of the growth and activity of Lactic acid bacteria used as starter and on the growth of Staph. aureus bacteria. Pure strains of Staph. aureus and Lactic acid bacteria was used the effect of Lactic acid bacteria (Streptococcus thermophilus and Lactobacillus bulgaricus) on the number of Staph. aureus was studied at 37 ◦C and 4◦C. The Lactic acid bacteria were inoculeted in milk for the manufacturing of yoghurt. The number of Staph. aureus was fixed then was decreased gradually according to time. And the decreasing number of this bacteria was clear when S. thermophilus and Lb. bulgaricus were used as a mixture starter, then when was used as a single starter, Lb. bulgaricus and S. thermophilus.
This research was conducted in the laboratories of Faculty of Agriculture – Damascus University Department of Food Sciences. The aim of this research was to study the inhibition effect of the growth and activity of Lactic acid bacteria as starter in yoghourt, on the growth of E. coli O157:H7 bacteria. A pure strains of E. coli O157:H7 and Lactic acid bacteria was used for this study. The effect of Lactic acid bacteria (Streptococcus thermophilus and Lactobacillus bulgaricus ) in the number of E. coli O157:H7 was studied in 37 ◦C and 4◦C. The Lactic acid bacteria was inoculeted in milk which was prepared to manufacture the yoghurt. The number of E. coli O157:H7 was gradually decreased with the time. The decline number of this bacteria was clear when S. thermophilus and Lb. bulgaricus were used as a mixture starter , then when Lb. bulgaricus was used as a single starter then S. thermophilus was used a single, In the other hand, the inhibition effect was weaker at 37 C and stronger on 4 C.
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