This study was conducted to evaluate the effect of seed Rhizobia-treating
and Cowpea severe mosaic virus (CPSMV) inoculation on bacterial nodulation
and nitrogen fixation in cowpea. CPSMV was isolated from infected plants,
College of Agriculture,
Abu-Ghraib, Baghdad, IRAQ, the Rhizobia was isolated
from nodules on roots of cowpea plants. The plants were inoculated by the
virus 2 weeks after germinations. Nodules on roots of Rhizobia-treated plants
were formed while no nodules were observed on non-treated ones. The mean
number of nodules was 24.93\ plant on virus inoculated plants compared to
59.86 nodules\plant on non-inoculated ones. Significant reduction in nodules
weight was induced by the virus, 0.62 gm on the virus inoculated plants
compared to 2.13gm on non-inoculated ones. It has been found that the
Rhizobia induced significant increase in nitrogen percentage at p=0.05 in the
foliage, 1.29% in plants non-treated with Rhizobia non-inoculated by the virus
compared with 2.502% in Rhizobia treated-virus inoculated plants and 2.550%
in Rhizobia treated non inoculated plants. The virus caused an increase in
nitrogen percentage, 1.877% compared to 1.29% in non-inoculated plants. The
Rhizobia caused an increase in pods number\plant, seed number\plant, and seed
dry weight (gm)\plant, 25.6, 114, 32.4 compared to 12.8, 70.6, 8.7 in non-treated
plants respectively. The treatment of virus inoculated plant by Rhizobia
induced a significant increase in these values, except with nitrogen percentage
in seeds, 16.4, 84.6, 10.4, 4.3 respectively.
Some species of the Syrian Flora were waned, other were extincted.
therefore, it was so nessesary to make a releve for restrict and classify the
survival species in order to put them under conserving & improvement genetic
program. The aim of this
research was to study the plant diversity in West
Homs; one of important basaltic region in Syria, and to concentrate on the wild
legumes and the Rhizobium strains which cohabit with. A plant survey was
carried out showed the existence of 365 species belong to 202 genera and 51
families, 15 species are endemic.12 species not observed previously in Syria but
are found in Lebanon were recorded,in addition another 3 species not listed at
all neither in Syria nor in Lebanon were mentioned. The geobotanical
attributes and the economical importance of the gathered species were
determined.as so as systematic keys of the most important families were
suggested...The root nodules of 10 species belong to 10 genera of wild legumes
were studied. Rhizobium strains were isolated and cultured in vitro. The
morphologic characters were studied microscopy. The pure strains were
reserved by refrigerator.
