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Register a new userSerologic detection of CagA positive Helicobacter pylori in patients with different gastroduodenal diseases
الكشف المصلي عن الملوية البوابية إيجابية الـCagA عند المرضى المصابين بأمراض معدية عفجية مختلفة
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هدفت هذه الدراسة إلى معرفة العلاقة بين ذراري الملوية البوابية (HP) إيجابية الـcagA و القرحة المعدية و العفجية و سرطان المعدة الغدي، شملت الدراسة 61 مريضاً مصاباً بعسر الهضم من المرضى المراجعين لقسم التنظير الهضمي في مشفى الأسد الجامعي في اللاذقية، في أثناء التنظير الهضمي العلوي لهم تم أخذ خزعات معدية لإجراء اختبار الـ Clo-test و الفحص النسيجي، ثم سحب الدم للمرضى إيجابتي الـ Clo-test لإجراء الاختبارات المصلية لـ HP و cagA بعد إجراء التنظير الهضمي العلوي و الفحص النسيجي تم توزيع المرضى على 4 مجموعات و هي التهاب المعدة المزمن (28 مريضاً) و القرحة المعدية (5 مرضى) و القرحة العفجية (17 مريضاً) و سرطان المعدة الغدي (11 مريضاً). كانت أضداد الـ CagA إيجابية عند 68.85% من مرضى الدراسة. و قد اختلفت نسبة إيجابية هذه الأضداد بشكل هام إحصائياً بين مجموعات المرضى الأربعة (0.006P = )، فكانت أعلى نسبة إيجابية بين مرضى سرطان المعدة الغدي (90.91%)، ثم القرحة العفجية بنسبة 88.24%، فالقرحة المعدية بنسبة 80%، بينما كانت أقل نسبة إيجابية بين مرضى التهاب المعدة المزمن (46.43%). كما وجدت دراستنا عدم وجود فروق هامة إحصائياً في عيار أضداد الـHP بين المجموعات الأربعة (P = 0.90 )، و لكن هذا العيار كان أعلى عند مرضى cagA+ بالمقارنة بــ cagA- (P = 0.05). خلصت الدراسة إلى وجود علاقة إيجابية بين الـcagA+ و القرحة الهضمية و سرطان المعدة الغدي في سوريا.
This study aimed to know the relation between cagA positive Helicobacter pylori (HP) strains and diseases such as gastric and duodenal ulcers and gastric adenocarcinoma. The study included 61 dyspeptic patients from gastrointestinal (GI) endoscopy department patients in Al-Assad university hospital in Lattakia. During upper (GI) endoscopy, gastric biopsy specimens were obtained for Clo-test and histologic examination. Blood was obtained from Clo-test positive patients for serologic tests of HP and cagA. After upper (GI) endoscopy and histologic examination, patients were divided into 4 groups: chronic gastritis (28 patients), gastric ulcer (5 patients), duodenal ulcer (17 patients) and gastric adenocarcinoma (11 patients). CagA antibodies were positive in 68.85% of patients. The difference in the proportion of CagA antibody positive between the four groups was highly significant (P= 0.006). The highest positive proportion was in gastric adenocarcinoma (90.91%) then duodenal ulcer (88.24%) then gastric ulcer (80%), while the lowest positive proportion was in chronic gastritis (46.43%). There was no difference in HP antibody levels between the four groups (P = 0.90) but these levels were significantly higher in cagA+ patients compared to cagA- (P= 0.05). This study found a positive relation between cagA+ and diseases such as peptic ulcer and gastric adenocarcinoma in Syria .
References used
KUSTERS, J. G.; VLIET, A. M.; KUIPERS, E. J. Pathogenesis of Helicobacter pylori Infection. Clin Microbiol Rev. 2006 July; 19(3): 449–490
BLASER, M. J.; ATHERTON, J. C. Helicobacter pylori persistence: biology and disease. J. Clin. Investig. 2004. 113:321-333
LEUNK, R. D.; JOHNSON, P. T.; DAVID, B. C.; KRAFT, W. G.; MORGAN, D. R. Cytotoxic activity in broth-culture filtrates of Campylobacter pylori. J. Med. Microbiol. 1988. 26:93-99
COVACCI, A.; CENSINI, S.; BUGNOLI, M.; PETRACCA, R.; BURRONI, D.; MACCHIA, G.; MASSONE, A.; PAPINI, E.; XIANG, Z.; FIGURA, N.; RAPPUOLI, R. Molecular characterization of the 128-kDa immunodominant antigen of Helicobacter pylori associated with cytotoxicity and duodenal ulcer. Proc. Natl. Acad. Sci. USA, 1993. 90:5791-5795
CHING, C. K.; WONG, B. C.; WOK, E. K.; ONG, L.; COVACCI, A.; LAM, S. K. Prevalence of CagA-bearing Helicobacter pylori strains detected by the anti-CagA assay in patients with peptic ulcer disease and in controls. Am. J. Gastroenterol. 1996. 91:949-953
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The study aims to determine the Seroprevalence of anti-H. pylori antibodies in patients with peptic complaints using indirect immunofluorescence assay (IFA), and compare serology with histological examination of biopsies from upper gastrointestinal e
ndoscopy to evaluate the sensitivity and specificity of this assay.
The Study sample included 120 participants divided into two groups: 69 patients with peptic complaints who underwent upper GI endoscopy in Al-Assad Hospital in Lattakia (average age 47 years old), and 51 volunteers without peptic complaints (average age 33 years old). The study was conducted in the period between February 2011 and March 2012. Indirect immunofluorescence assay (IFA) was used to study the seroprevalence of anti-H. pylori antibodies and fluorescence intensity in the two groups.
The results showed that 63 of the patients group (91.3%) and 39 of volunteers (76.47%) are anti-H. pylori antibodies positive. The comparison of serology with histological examination of biopsies showed a sensitivity and specificity of 78% and 80% respectively in the presence of moderate and strong degree of fluorescence. The results reveal the importance of IFA as a non-invasive screening test besides the endoscopy, and the utility of this technique for the epidemiologic studies in a local setting.
The aim of this study is to determine the incidence of helicobacter pylori
(HP) in patients with chronic gastroduodenitis and to compare the clinical
presentations between patients with positive HP chronic gastroduodenitis
and those with negative
HP. Also, To compare the endoscopic findings
between patients with HP positive gastroduodenitis and HP negative
children.
Gastroduodenoscopy was done for every child with abdominal pain
admitted at the children hospital-Damascus University between 2002-2003.
Mucosal biopsy was taken from every patient who had gastroduodenitis
diagnosed by gastroduodenoscopy.
The percentage of patients with gastroduodenitis having HP positive
erosions was 48.38 %.
Higher percentage of HP positive patient was more present in those with the
erosion and sub-atrophy forms.
Objective: To study the relationship of H. pylori infection with short stature in
children with upper gastrointestinal endoscopy with dudenal biopsy with the clotest test on
the antrum biopsy for three years from the beginning of 2011 until the end
of 2013 and to
study the relationship with Age and sex
Methods: The complete records of all children admitted to the pediatric ward at Al-
Assad University Hospital and upper gastrointestinal endoscopy was performed for the
first time with a dudenal biopsy and a histological study with rapid urease test on the
antrum biopsy during the years 2011-2012-2013. Patients were divided into two groups :
the infected group and the non-infected group based on the clotest result.
The differences in standard deviations of the lengths of the two groups were studied.
The mean lengths were not studied because of the age difference between the two groups.
Results: The number of patients in the study sample was 180 patients with ages from
6 months to 14 years. Median age was (6) years. The distribution was 95 (52.8%) males
and 85 (47.2%) females. Weight loss, short stature and abdominal pain were the main
reasons for endoscopy. The rate of helicobacter pyloi infection was 76 children from 180
and the distribution was 51.3% for males and 48.7% for females. There were no
statistically significant differences in the distribution of infection by sex. H. pylori
infection was higher in older ages. The age groups were (3-6 years) and 6-9 years the
biggest. Weight loss and shortness of stature were more pronounced in the group of
patients compared to non-infected patients, and there was a statistically significant
difference in the standard deviation of lengths in children with H. pylori compared to noninfected
patients. There were no significant statistical differences in weight or gender.
20 Syrian adult patients from both sexes underwent study at Al-Mouassat
Hospital diagnosed with primary gastric E1 MALT lymphoma, Associated
with H.P. and treated only with H.P. eradication. This treatment led to H.P.
eradication in all patients (
100%), and complete regression of 85% of
Lymphoma.
Relapse did not appear during follow up period which exceeded 5 years in
all patients, 15% cured after chemotherapy without surgery nor
radiotherapy.
Background& Objective: Onychomycosis is a frequent nail disease caused by dermatophytes, yeasts, and
non-dermatophyte molds. Far more than being a simple cosmetic problem, infected nail serves as a
chronic reservoir, which can give rise to repeated
mycotic infections.
This study aim to identify the common fungal species which are responsible for onychomycosis in
clinically suspected patients of onychomycosis in Dermatology and Venereal Diseases Hospital in
Damascus between October 2010 and July 2011.
Materials & Methods: Both direct microscopy and culture of the nail material were performed to identify
the causative fungi of onychomycosis.
Results: Culture positivity was obtained in 65 of the 76 clinically suspected samples, etiological fungal
agents were (%61.5) dermatophytes, (24.6%) yeasts, and (13.9 %) non-dermatophyte molds . The isolated
fungal species were (43.1%) Trichophyton Rubrum and (16.9%) T. Interdigitale (1.5%) T.Verrucosum
Aspergillus Versicolor which was the most frequent non dermatophyte molds. Females were affected in
fingernails more frequently than males, while males were affected in toenails more frequently than
females, and in both sexes those most infected were between 20-40 years of age.
Conclusion: Dermatophytes, in particular T. rubrum, but also T. Interdigitale, are the most frequently
isolated causative agents in onychomycosis in patients seen in Dermatology and Venereal Diseases
Hospital in Damascus . In addition, yeasts may be isolated relatively frequently, while molds are
uncommon.
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