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Register a new userThin layer chromatography–image analysis method for the determination of monosodium glutamate in food
استعمال تقنية الفصل الكروماتوغرافي على طبقة رقيقة-تحليل الصورة لمعايرة المونوصوديوم غلوتامات في بعض الأغذية
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تم تطوير طريقة كروماتوغرافيا على طبقة رقيقة – تحليل الصورة (TLC-image analysis) بسيطة و قليلة الكلفة لتحديد و لقياس كمي سريع للمونوصوديوم غلوتامات (MSG) في بعض عينات الغذاء، و تحقق الفصل الكروماتوغرافي على صفائح السيليكاجيل TLC، باستخدام البوتانول: حمض الخل الثلجي: الماء (v/v/v 1:3:5) كطور متحرك، و النهيدرين ككاشف للبقع. أنجز تحليل صورة للصفيحة الممسوحة ضوئياً لتحديد كمية (MSG)، و تم تقويم الطريقة و ضبطها فوجد أنها تتمتع بصحة النتائج و النوعية و مناسبتها لتحليل MSG في بعض عينات الغذاء.
A simple and low cost thin layer chromatographic (TLC)-image analysis method was developed for rapid determination and quantification of monosodium glutamate (MSG) in some food samples. Chromatographic separation of MSG was achieved on silica gel TLC plates, using n-butanol:glacial acetic acid:water (5:3:1, v/v/v) as the mobile phase and ninhydrin for spot detection. Image analysis of the scanned TLC plate was performed to quantify the amount of MSG, the method was validated and found to be accurate specific reliable and convenient for the analysis of MSG in some food sample.
References used
Jinap, S; Hajeb, P. Glutamate. Its applications in food and contribution to health. Appetite 55, 2010, 1–10
RAITEN, D. J; TALBOT, J. M; FISHER, K. D. Executive Summary from the Report: Analysis of Adverse Reactions to Monosodium Glutamate (MSG). J. Nutr U.S.A, 125(1), 1995, 2893-2906
ATI, Kh. A; MOHAMMED, S; SAAD, A. M; AND MOHAMED, H. E. Response of broiler Chicks to dietary Monosodium Glutamate. Pakistan vet. J, 29 (4), 2009, 165- 168
SINGH, K; PUSHPA, A. Alteration in some antioxidant enzymes in cardiac tissue upon Mono Sodium Glutamate (MSG) administration to adult male, Indian journal of Clinical bio chemistry, India, 20(1), 2005, 43-46
BASU, A. K; CHATTOPADHYAY, P; R OYCHUDHURI, U;CHAKRABORTY, R. Development of biosensors based on immobilized L-glutamate oxidase for determination of monosodium glutamate in food, Indian Journal of Experimental Biology, India, 44, 2006, 392-398
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