Bone healing is a complicated biological mechanism that is affected by many hormonal, nutritional, and hemal factors. The term Biomechanical theory of bone healing is a concept that is created by us and is used for the first time to identify and stud
y the influence of mechanical rules on the bone tissue so we can study and imitate its mechanical response depending on the rules of balance and movement. We ensure that bone healing is just adaptation to auto bone forming mechanism in the surrounding mechanical site. The term is more comprehensive and accurate than the bio-compression term which was used before; and all of those are confirming the criteria to evaluate the efficacy of fixators used in orthopedic surgery.
Cancer stem cells in solid tumors are a "rare" group of cells and
their role in carcinogenesis has been proven during the past few
years. The pursuit for specific oral epithelial stem cell surface
markers leads to the identification of markers su
ch as CD44
and Bmi-1. The combination between CD44 which has been
considered a potential prognostic marker for cancer stem cells in
Head and Neck Squamous Cell Carcinoma (HNSCC), and Bmi-1
the pluripotency stemness marker may lead to identify the cells
sharing the properties of both markers and could give a closer look
into its appearance and density in the tissue. The aim of this study
was to study the percent of cells stained positive for both markers
and the histological grade of (HNSCC) and the anatomic location
of the tumor. Statistical analysis showed that higher density of
positive stained cells for both markers was in the severe grade of
the tumor, whereas the second higher ratio was in the mild grade
then the moderate , there was no significant association between the density of cells and the anatomical location. Our results suggest that the two markers together could be used as prognostic factors for patients with even mild grade SCC, which might help in prediction of recurrence.
Stem cells have unique capability to differentiate into many cell types that can
normally replace the loss in some cells of the body due to tissue injury. Umbilical cord blood (UCB) and
umbilical cord (UC) are the two main sources for hematopoietic
stem cells (HSCs) and mesenchymal stem
cells (MSCs), respectively, which constitutes the basis for stem cell banks that have been established
worldwide and very recently in Syria. Research in our region has mainly focused on cell storage and
freezing protocols, and only few studies were conducted to prove the ability of the stored cells to
differentiate into their destined lineages. This study aimed to test the potential of cryopreserved MSCs
isolated from an umbilical cord taken from new delivery at Maternity University Hospital in Damascus, to
differentiate into various types of cells in response to growth and induction factors specific to cell lineages.
This research aims at establishing
and testing protocols for isolation, in vitro proliferation, phenotyping, and
differentiation of MSCs embedded in umbilical cord tissues of Syrian
newborns.
MSCs were isolated from two caesarian births depending
on their
adherence characteristic on plastic surfaces, and cells were cultured in MSC
medium for their growth and proliferation. Cell phenotyping was performed by
flow cytometry using fluorescent monoclonal antibodies specific to MSCs’
surface markers. Cultured cells were passed several times and a portion of
these cells was cryopreserved in liquid nitrogen, and cell viability was assessed.
Differentiation of these MSCs into adipocytes was conducted using culture
medium Indomethacin and hydrocortisone.
