The research aimed to evaluate the genetic diversity and the genetic
relationship between 16 Hordeum vulgare genotypes (variaties and new lines),
using SSR markers. 32 primer pairs were used in the analysis, five of them
amplified primers monomorp
hic alleles, while the remaining primers detected
different alleles. A total of 126 polymorphic alleles were revealed by the 27
primer pairs. The number of different alleles detected on alocus ranged from 2
to 11with a mean of 4.66 alleles per locus. The polymorphic information content
(PIC) values ranged from 0.21 to 0.88 with a mean value of 0.49, and the gene
diversity ranged from 0.23 to 0.89 with a mean value of 0.53.The dendrogram
was established using SSR data clustered into three major groups. The smallest
genetic relationship was observed between line39 and line38 . The results proved
the efficiency of SSR markers in the estimation of genetic diversity and in the
clustering of the barley genotypes in Syria.
Seven plant samples were collected from some locations of Syrian juniper
(Juniperus drupacea Labill.) in Syria for molecular characterization and to
determine the genetic relationships between them using ISSR technique (Inter
Simple Sequence Repea
ts). Twenty three ISSR primers were used for this
purpose, twelve primers showed polymorphism between studied samples and
gave 89 bands, with polymorphism percentage of 95.5%. The band number
resulted from each primer ranged between 4 bands for primers ISSR5 and
ISSR9, and 12 bands for the primer ISSR1, with an average of 7.42 bands per
primer. The minimum polymorphic percentage was 25% for primer ISSR9,
and the maximum polymorphic percentage was 100% for the all primers
except the primer ISSR9. The study showed correlation between the genetically
converged samples and the collection sites (geographic correlation), the highest
genetic relationship (93%) was within Latakia samples (Komat Alnabi Yonis –
Jobet Bergal) and the lowest genetic relationship (42%) was between samples
from Hamah (Jeb Alahmer) and Latakia (Almakamat) which refers to high
genetic variation. The cluster analysis showed that the samples from nearby
locations were gathered.
